Journal of Tissue Engineering and Reconstructive Surgery ›› 2009, Vol. 5 ›› Issue (1): 7-11.doi: 10.3969/j.issn.1673-0364.2009.01.003

• Original article • Previous Articles     Next Articles

Expression of 3β-Hydroxysteroid Dehydrogenase in Rat Leydig Cells Cultured by Differential Adhesion In Vitro

BI Hongda1,WANG Xiaoyun1,ZHOU Guangdongi2,LIU Wei2,LI Ming1,XING Xin1   

  1. 1 Department of Plastic Surgery;Changhai Hospital;Second Military Medical University;Shanghai 200433;China. 2 Shanghai Laboratory of Tissue Engineering;Shanghai Ninth People′s Hospital;Shanghai Jiaotong University School of Medicine;Shanghai 200011;China.;
  • Published:2020-07-23

Abstract: Objective To investigate the constitution of cells population, changes in 3β-hydroxysteroid dehydrogenase (3β-HSD) expression and androgen productivity in rat Leydig cells cultured by differential adhesion during 4 days primary culture. Methods Testes from 7 days and 3 weeks old rats were harvested, then Leydig cells were isolated by using collagenase dispersion, stainless steel mesh infiltration and differential adhesion. 3β-HSD expression in cultured cells was observed by immunohistochemistry and flow Cyt...

Key words: Leydig Cell, Differential Adhesion, Testosterone, 3β-hydroxysteroid dehydrogenase, Tissue Engineering

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