Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene

doi:10.3969/j.issn.1673-0364.2009.04.004

Journal of Tissue Engineering and Reconstructive Surgery ›› 2009, Vol. 5 ›› Issue (2): 75-78.doi: 10.3969/j.issn.1673-0364.2009.04.004

• Original article • Previous Articles Next Articles

Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene

BIAN Suyan¹,GAI Luyue¹,YE Ping¹,YANG Yuefeng²,WANG Rongliang²,WANG Hua²,GUO Zikuan²,Wang Lisheng²

1 Second Department of Cardiology;Southern building;General Hospital of PLA;Beijing 100853;China.2 Department of Experimental hematology;Institute of Radiation Medicine;Academy of Military Medical Sciences;Beijing 100850;China;

Published:2020-07-23
Contact: 自然科学基金青年基金面上项目(30400182) 国家高技术发展计划(863)项目(2007AA02Z454)

Abstract

Abstract: Objective To construct recombinant retroviral vector carrying firefly luciferase reporter gene(fluc) for further study of bioluminescence imaging in vivo.Methods Target gene-fluc was obtained by cutting firefly luciferase reporter gene(fluc) vector pGL3-Basic using restriction enzyme digestion.Then fluc gene was sub-cloned into retroviral vector pLXSN.GP+E86 and PA317 pakage cells were infected by the obtained recombinant retroviral vector pL(fluc)SN with ping-pong infection assay and screened with G418 res...

Key words: Firefly luciferase reporter gene（fluc） vector, pGL3-Basic, Recombinant retroviral vector, pLXSN, Construction

CLC Number:

Q784

BIAN Suyan,GAI Luyue,YE Ping,YANG Yuefeng,WANG Rongliang,WANG Hua,GUO Zikuan,Wang Lisheng. Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(2): 75-78.

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Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene

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