Abstract: 　In recent years, 3D printing of "bioactive scaffolds" has become a research hotspot. Although great progress has been made in 3D printing of bioactive scaffolds, in the printing process of bioactive scaffolds, many factors will lead to cell damage or even cell death, mainly including the following reasons: 1) Different printing methods can produce different types of cell damage and damage degrees, such as heat damage, extrusion damage, shear damage and tensile stress damage, etc. 2) Whether there is a coating on the receiving substrate, the thickness of the coating and the type of raw materials, etc. The coating on the receiving substrate can play a dual role of cell culture medium and collision buffer, both of which will have a certain degree of influence on cell damage, and the degree of cell damage directly affects the biological activity of the 3D-printed bioactive scaffold. In this paper, the cell damage in the printing process of bioactive scaffolds was reviewed based on the latest research progress.

Key words: 3D printing, 　Scaffolds, 　Cell damage