Journal of Tissue Engineering and Reconstructive Surgery ›› 2024, Vol. 20 ›› Issue (2): 176-.

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Research on preparation and biocompatibility of chitosan-collagen scaffold

  

  • Online:2024-04-30 Published:2024-05-14

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Abstract:

Objective To prepare osteochondral tissue engineering materials by mixing chitosan （CS） and collagen
（Col）， and their physical properties and biological safety were tested， so as to provide a biological scaffold material for the
repair of osteochondral injury. Methods The three groups of mixed scaffolds of CS and Col were made by vacuum freeze
drying method in different proportions. The porosity， water absorption expansion rate， hot water dissolution rate and
mechanical properties of the scaffolds were detected. The scaffold structure was observed by scanning electron microscope，
and its cytotoxicity and biocompatibility were detected by CCK-8 method， F-actin staining method， and Live/Read cell
staining method. Results The scaffolds were white and regular cylinders， and all three groups of scaffolds had good
porosity， water absorption expansion rate， hot water dissolution rate and mechanical properties. Scanning electron microscopy
showed that all three groups of scaffolds had good porous grid structure， and the physical properties were the best when CS︰
Col was 1︰3. The results of CCK-8 method showed that， compared with the control group， there was no obvious growth
inhibition in the three groups within a certain time （P>0.05）
. The results of F-actin staining showed that the cells in each
group were uniform in size， the cytoskeleton was regular in shape， and the nucleus was not stained and broken. After DAPI
staining， it was found that the cells grew and distributed well in the scaffold. Live/Read cell testing revealed that all three
groups of scaffolds had no cytotoxicity to cells， and there was no significant difference in cell viability among the three groups
of scaffolds （P>0.05）
. Conclusion CS-Col composite scaffold has good biocompatibility and physical properties， and it is a
potential tissue engineering material for osteochondrosis.

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