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| Obtaining of Transgenic Glyphosate-Resistant Soybean |
Yun-yun CUI( ), Yue-ping CAO() |
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| Guide |
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Abstract Soybean (Glycine Max L.Merrill) is an important grain and oil crop in China.However its production has been seriously reduced due to large scale soybean import from US Methods of biotechnology have been applied to soybean for improvement of the agronomic traits and the quality of the product.One such agronomic trait important in soybean production is herbicide tolerance, in particular, tolerance to glyphosate herbicide.In this study, Agrobacterium-mediated method was used to introduce G10-EPSPS into soybean Dongnong 50.Three transgenic soybean plants were obtained.The obtained transgenic soybean plants were all resistant to glyphosate and 1 event expressed the resistance stably in T0, T1 and T2 generations.Molecular analysis by PCR and Western blot verified that G10-EPSPS was integrated into the soybean genome and inherited stably in T0, T1 and T2 generations.It would be helpful for further study and soybean breeding.
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Received: 23 February 2015
Published: 08 March 2016
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Different periods of soybean transformation Note: A:co-cultivation; B:induction; C, D:elongation; E:T0 transgentic plant
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PCR detection for T0. Note: M: Marker DL2000; 1, A, A1, A2:Transformation plants; CK+:Positive control; CK-:Negative control
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The transgenic plant at mature period
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Western blot analysis of different tissues Note: M: Marker; 1:Negative control; 2:root; 3:stalk; 4:leaf; 5:petal; 6:pod; 7:Positive control
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Identification of some transgenic glyphosate - resistant soybean T2 plants Note: A, B, C:transgenic T2 plants; CK:Negative control
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2016, Vol. 34 
