诊断学理论与实践 ›› 2018, Vol. 17 ›› Issue (06): 640-644.doi: 10.16150/j.1671-2870.2018.06.004

• 论著 • 上一篇    下一篇

肺炎克雷伯菌中携带碳青霉烯酶KPC-2基因的质粒分型

沈平华1, 张贤华1, 蒋晓飞2, 陈慧芬1   

  1. 1.同济大学附属第一妇婴保健院检验科, 上海 200040;
    2.复旦大学附属华山医院检验科, 上海 200040
  • 收稿日期:2018-09-05 出版日期:2018-12-25 发布日期:2018-12-25
  • 通讯作者: 陈慧芬 E-mail: weedeng@sina.com

Plasmids typing of genes carrying blaKPC-2 in Klebsiella pneumoniae isolated at a tertiary hospital in Shanghai

SHEN Pinghua1, ZHANG Xianhua1, JIANG Xiaofei2, CHEN Huifen1   

  1. 1. Department of Laboratory Medicine, Shanghai First Maternity and Infant Hospital, Tongji University, Shanghai 200040, China;
    2. Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2018-09-05 Online:2018-12-25 Published:2018-12-25

摘要: 目的:观察华山医院临床耐碳青霉烯类肺炎克雷伯菌中携带blaKPC-2质粒的分型情况, 以了解其流行变迁。方法:共收集华山医院临床连续不重复的blaKPC-2阳性碳青霉烯类耐药肺炎克雷伯菌38株, 对其进行多位点序列分型(multilocus sequence typing, MLST)分型, 并用松弛酶对质粒进行分型。结果:8株肺炎克雷伯菌所携带的blaKPC-2阳性质粒属于MOBP3亚型, 另30株均为MOBF12亚型。同时, 进化树显示, 本批菌株所含有的MOBF12亚型质粒位于2个不同分支上, 提示该批菌株的MOBF12亚型质粒具有不同的生物来源。结论:首次运用松弛酶对肺炎克雷伯菌所携带的blaKPC-2阳性质粒进行分型, 发现其中8株属于MOBP3亚型, 30株为MOBF12亚型, 且MOBF12亚型菌株具有不同的生物来源, 反映了华山医院临床耐碳青霉烯类肺炎克雷伯菌中携带blaKPC-2质粒的多样性。

关键词: 肺炎克雷伯菌, blaKPC, 质粒, 松弛酶

Abstract: Objective: To investigate the plasmids typing of genes carrying blaKPC-2 in carbapenemase resistant Klebsiella pneumoniae for exploring its epidemiological evolution. Methods: Thirty-eight carbapenem -resistant Klebsiella pneumoniae isolates obtained consecutively from Huashan Hospital were collected, all carrying blaKPC-2-bearing plasmids. All the strains were subjected to multilocus sequence typing (MLST). Relaxase analysis was used to classify these plasmids. Results: Eight of the blaKPC-2-bearing plasmids belonged to the MOBP3 and 30 blaKPC-2-bearing plasmids belonged to the MOBF12. Phylogenetic analysis revealed that the MOBF12 plasmids were located at two different branches, respectively, suggesting they had different biological origin. Conclusions: It is the first time that relaxase has been used for plasmids typing of genes carrying blaKPC-2 in carbapenemase resistant Klebsiella pneumoniae, and found 8 belonged to the MOBP3 and 30 belonged to the MOBF12, and the MOBF12 having different biological origin, denoting the epidemiological evolution of blaKPC-2 bearing plasmid in carbapenemase resistant Klebsiella pneumoniae.

Key words: Klebsiella pneumoniae, blaKPC, Plasmid, Relaxase

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