关键词: miR-146b-3p, 增龄, 脂肪前体细胞, 棕色脂肪

Abstract: Objective: To investigate correlation between brown adipogeneic capacity of preadipocytes and ageing and the related mechanism in mice. Methods: Preadipocytes derived from various age mice were cultured in vitro, and oil red O staining was used to identify the brown adipogenic capacity. The expression of miR-146b-3p in young and old preadipocytes was detected by real-time quantitative PCR. Then miR-146b-3p inhibitors were transfected into young preadipocytes, while miR-146b-3p mimics were transfected into the old, and the relative mRNA levels of PLIN, FABP4, UCP-1, APN were measured by real-time quantitative PCR. Results: Preadipocytes of aged mice showed a weaker capacity of brown adipogenic capacity than that of the young mice, and the expression of miR-146b-3p in the preadipocytes of the aged group was also decreased. Overexpression of miR-146b-3p can improve the brown adipogenic capacity of preadipocytes in aged mice. However, the brown adipogenic capacity of the young declined with the decrease of miR-146b-3p expression. Conclusions: The brown adipogenic capacity of preadipocytes decreases with age, and miR-146b-3p might be one of the targets.

Key words: miR-146b-3p, Ageing, Preadipocytes, Brown adipocytes