微小RNA-337-3p在口腔鳞状细胞癌中的表达及临床意义

doi:10.16150/j.1671-2870.2020.05.012

摘要/Abstract

摘要：

目的： 探讨微小RNA（microRNA, miRNA）-337-3p在口腔鳞状细胞癌（oral squamous cell carcinoma, OSCC）组织中的表达及其临床意义。方法： 收集20例OSCC患者的术后标本,检测癌组织及癌旁正常组织中的miRNA-337-3p表达水平,分析其表达水平与患者临床病理参数间的相关性;采用划痕试验评估miRNA-337-3p对OSCC细胞株HN30迁移能力的影响,探索可能的分子机制。结果： 与癌旁正常组织相比,OSCC组织中的miRNA-337-3p呈显著低表达（P<0.000 1）,且其表达水平与OSCC患者淋巴结转移状况显著相关（P<0.05）;与未发生淋巴结转移的患者（N₀）相比,发生淋巴结转移（N₁+N₂）患者的OSCC组织中miRNA-337-3p表达水平显著降低（P<0.05）。在HN30细胞中,过表达miRNA-337-3p的细胞迁移能力降低,抑制miRNA-337-3p表达,则细胞迁移能力上升。miRNA-337-3p可抑制基质金属蛋白酶14 （matrix metalloproteinase-14,MMP-14）蛋白的表达。结论： miRNA-337-3p在OSCC中发挥抑癌作用,其可能具有抑制OSCC细胞侵袭及转移的功能,其有望成为OSCC侵袭、转移的早期诊断标志物及为预后监测提供帮助。

关键词: 口腔鳞状细胞癌, 微小RNA, microRNA-337-3p, 转移

Abstract:

Objective: To investigate the expression of microRNA (miRNA)-337-3p in oral squamous cell carcinoma (OSCC) and its clinical significance. Methods: The expression of miRNA-337-3p in OSCC cancer and paracancerous tissue of 20 OSCC patients was determined, and the correlation of miRNA-337-3p expression with clinical-pathological parameter was analyzed. Scratch test was performed to detect the effect of miRNA-337-3p on migration ability of OSCC cell line HN30 cells, and the possible molecular mechanism was explored. Results: Expression of miRNA-337-3p in OSCC was significantly lower than that in paracancerous tissue(P<0.000 1), and was correlated significantly with the status of lymph node metastasis (P<0.05). Patients with lymph node metastasis (N₁+N₂) had a lower expression level of miRNA-337-3p than those without lymph node metastasis (N₀). Overexpression of miRNA-337-3p in HN30 cells inhibited cell migration, whereas suppression of miRNA-337-3p led to increase of cell migration. miRNA-337-3p could inhibit the expression of matrix metalloproteinase-14 (MMP-14). Conclusions: miRNA-337-3p plays a suppressive role in OSCC, inhibitingthe invasion and metastasis of OSCC cells, and is expected to be used as a biomarker for assessing the invasion and metastasis of OSCC and helping the monitoring of prognosis.

Key words: Oral squamous cell carcinoma, microRNA, microRNA-337-3p, Metastasis

中图分类号:

R730.261

张星明, 顾文莉. 微小RNA-337-3p在口腔鳞状细胞癌中的表达及临床意义[J]. 诊断学理论与实践, 2020, 19(05): 510-515.

ZHANG Xingming, GU Wenli. Expression of microRNA-337-3p in oral squamous cell carcinoma and its clinical significance[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(05): 510-515.

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特征	例数（n=20）	miRNA-337-3p		P值
特征	例数（n=20）	高表达(n=10)	低表达(n=10)	P值
性别				0.656
男	10	4	6
女	10	6	4
年龄（岁）				0.650
≤60	8	3	5
>60	12	7	5
吸烟^a）				0.582
是	4	1	3
否	16	9	7
饮酒^b）				0.211
是	3	0	3
否	17	10	7
T分期				0.350
T₁+T₂	13	5	8
T₃+T₄	7	5	2
N分期				0.029^*
N₀	7	6	1
N₁+N₂	13	4	9
病理分级				0.650
Ⅰ	8	5	3
Ⅱ+Ⅲ	12	5	7