Establishment of a detection method for tumor suppressor gene HIC-1 methylation in cell-free DNA and its significance in diagnosis of breast diseases

doi:10.16150/j.1671-2870.2019.02.005

Abstract

Abstract:

Objective: To establish a detection method for gene hypermethylated in cancer 1（HIC-1） methylation in cell-free DNA in peripheral blood and to evaluate its significance in diagnosis of benign and malignant breast tumors. Methods: The cell free DNA was extracted from 45 blood samples including breast disease patients and healthy volunteers. These DNA were treated with bisulfite and using Bisulfite Sequencing PCR(BSP) method to detect the methylation level of the 16 CpG sites in HIC-1 gene promoter region -636—-424 bp. Results: Of the breast cancer group, the methylation trend was obvious among the 4 sites in -636—-617 bp. Its average methylation rate was 22.6%. Frequency of methylation of HIC-1 gene promoter region was significantly higher in 25 patients with breast cancer compared with that in 10 benign tumor samples (22.6% vs 8.5%, P<0.05) and 10 healthy control samples (22.6% vs 8.3%, P<0.05). The ROC curve analysis showed that the area under curve（AUC） was 0.794, indicating a certain diagnostic value for breast cancer. Conclusions: The detection of HIC-1 gene promoter methylation in cell-free DNA has certain clinical significance in the diagnosis of breast cancer.

Key words: Breast cancer, Tumor suppressor gene, Hypermethylated in cancer 1, Methylation detection, Cell-free DNA

CLC Number:

R737.41

ZHONG Ming, ZHAO Feng, WU Yan, PEI Wenjiang, GAO Hang, GUO Shanyu, DAI Qiancheng, ZHANG Wei. Establishment of a detection method for tumor suppressor gene HIC-1 methylation in cell-free DNA and its significance in diagnosis of breast diseases[J]. Journal of Diagnostics Concepts & Practice, 2019, 18(2): 144-148.

Figures/Tables 5

References 19

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指标	例数(n)	甲基化频率(%)	P值
年龄（岁） ≥50 <50	18	23±14 22±16	0.961
肿瘤直径 ≥2 cm <2 cm	15 10	21±15 24±14	0.610
临床分期 Ⅰ+Ⅱ Ⅲ+Ⅳ	22 3	22±13 23±25	0.921
腋窝淋巴结阳性阴性	5 20	27±19 21±13	0.419
ER 阳性阴性	19 6	21±14 26±17	0.476
PR 阳性阴性	18 7	21±14 26±16	0.499
HER2 阳性阴性	11 14	18±13 26±14	0.155