Abstract: Objective: To investigate the performance of 3 types of automatic immunohistochemical stainer(Leica Bond-Max, Dako Omnis and BenchMark Ultra automatic immunohistochemical stainer) in pathological diagnosis of invasive ductal carcinoma of breast. Methods: Paraffin-embedded tissue blocks from 30 patients with infiltrating ductal carcinoma were collected,and 12 sections with a thickness of 3 μm were excised from each paraffin-embedded tissue block. After being stained with these three types of automatic immunohistochemical stainer, the specimens were examined to determine the expressions of estrogen receptor(ER）, progesterone receptor (PR）, human epidermal growth factor receptor 2（HER-2） and Ki67. Positive rates of above mentioned parameters between specimens prepared with these three different stainers were compared. Results: For Leica Bond-Max, the cases with positive ER(10%-99%, +~+++）, PR(1%-99%, +~+++）, HER-2（+~+++） and Ki-67（5%-90%, +）were 18, 14, 29 and 30 cases, respectively, by Dako Omnis were 18, 12, 28 and 30 cases, respectively, and by BenchMark Ultra were 18, 12, 28 and, 30 cases, respectively. There were no significant differences in positive expressions of these parameters in specimens prepared with these 3 types of automatic immunohistochemical stainer(P>0.05). Conclusions: Pathologic results of ER, PR, HER-2 and Ki-67 expression in breast carcinoma tissue were similar when specimens were prepared with these three types of automatic immunohistochemical stainer, indicating that specimens could be prepared with any type of above mentioned automatic immunohistochemical stainer.

Key words: Automatic immunohistochemical stainer, Breast cancer, Pathological diagnosis