Effect of Haemophilus influenzae colonizing lower respiratory tract on airway inflammation and its signaling pathway in asthmatic mice

doi:10.16150/j.1671-2870.2020.01.010

Abstract

Abstract:

Objective: To observe the effect of Haemophilus influenzae colonizing lower respiratory tract on airway inflammation in asthmatic mice and study the related signaling pathway. Methods: Thirty-two C57/B6 mice were sensitized and challenged with ovalbumin (OVA) to establish a mice model of chronic asthma (AC group), and then half of them received intratracheal injection of Haemophilus influenza coated in agar beads to built a mice model of chronic asthma with airway Haemophilus influenza colonization (AS group). Besides, 16 C57/B6 mice were treated with intratracheal injection of Haemophilus influenza (NS group) and 16 C57/B6 mice received intratracheal injection of 0.9% NaCl (NC group). Sixty-four TLR4^-/- mice were treated and grouped as C57/B6 mice. Mice including C57/B6 and TLR4^-/- mice were sacrificed at 7 and 14 days after intratracheal injection, and serum tumor necrosis factor α(TNF-α) content, total number of cells in bronchoalveolar lavage fluid( BALF), and the expression of MyD88 and NF-κB in lung tissue were measured. The indice in AS group were compared with those in the control groups (AC, NS and NC) at corresponding time. Results: The serum level of TNF-α, total number of cells in BALF, and expression of MyD88 and NF-κB in lung tissue were higher in AS group than those in NC and NS group (both in C57/B6 mice and TLR4^-/- mice). Compared with C57/B6 wild-type mice, TLR4^-/- mice in AS group had lower levels of serum TNF-α, decreased numbers of cells in BALF, and down-regulated expression of MyD88 and NF-κB in lung tissue. Conclusions: Lower airway colonization with Haemophilus influenzae may aggravate airway inflammation of asthma by activating the TLR4-MyD88 pathway and promoting expression of NF-κB, which may be one of the important mechanisms of asthma.

Key words: Haemophilus influenza, Asthma, Airway inflammation, Signaling pathway

CLC Number:

R563.25

KANG Jianqiang, DONG Yangyang, YANG Ling, SONG Zhen, FAN Jiaying. Effect of Haemophilus influenzae colonizing lower respiratory tract on airway inflammation and its signaling pathway in asthmatic mice[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(1): 44-49.

Figures/Tables 8

References 21

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分组	第7天		第21天
分组	C57/B6组	TLR4^-/-组	C57/B6	TLR4^-/-组
NC	29.00±2.64	31.00±3.64	28.69±1.71	29.33±126
NS	200.67±9.50^a)	109.35±10.36^a),c)	100.67±9.5^a)	53.10±2.04^a),c)
AC	120.67±20^a)	130.76±8.2^a)	119.67±9.9^a)	129.02±10.1^a)
AS	313.67±6.4^a),b)	200.61±9.52^a),b),c)	151.67±14.4^a)	137.3±7.53^a)

分组	第7天			第21天
分组	C57/B6组	TLR4^-/-组	C57/B6组	TLR4^-/-组
NC	10.83±1.96	11.60±8.70	12.49±6.72	11.60±0.17
NS	14.61±3.59^a)	15.89±1.41	19.57±5.25	18.58±1.79
AC	14.46±3.65	18.02±4.23^a)	14.47±3.84	17.64±5.78
AS	150.59±66.94^a),b)	28.26±5.22^a),b),c)	76.09±22.64^a),b)	38.98±3.13^a),b),c)