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25 February 2020, Volume 19 Issue 1 Previous Issue    Next Issue

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CT features of 2019 novel coronavirus pneumonia CAO Qiqi, QIN Le, ZHOU Huijuan, YANG Zhitao, SU Wenting, YANG Wenjie, CHENG Zenghui, LU Yong, YAN Fuhua, PAN Zilai 2020, 19 (1):  16-19.  DOI: 10.16150/j.1671-2870.2020.01.005 Abstract ( 878 )   HTML ( 6 )   PDF (655KB) ( 137 )   Objective: To analyze the CT features of patients with 2019 novel coronavirus （2019-nCoV） pneumonia, and explore the value of CT examination in the diagnosis. Methods: The clinical data of patients with fever and 2019-nCoV detected by real-time fluorescence RT-PCR from January 20 through February 10, 2020 were retrieved and imaging findings on CT were retrospectively reviewed. Results: Of all 12 patients with 2019-nCoV pneumonia, all had pulmonary infiltrations, involving multiple lung lobes and segments. Lesions on CT image showed GGO in all patients (12/12), predominantly located in the peripheral zone, with local reticular pattern within GGO and air bronchogram identified in 10 and 9 cases respectively. The patchy consolidation accompanying GGO presented in 8 cases. The lymphadenopathy and pleural effusion, however, were absent in all patients. Conclusion: For patients with novel coronavirus pneumonia within the first week of illness, the multifocal and patchy peripheral pure GGO, predominantly in the lower lung,is the most common findings on CT images. The extensive GGO with mild consolidation on CT scans is highly suspicious of 2019-nCoV infection and may prompt early treatment and prompt isolation in early outbreak. Figures and Tables | References | Related Articles | Metrics

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Phenotype and genotype diagnosis of 6 pedigrees with inherited coagulation factor Ⅹ deficiency LIU Jiajie, SHAO Yanyan, LIANG Qian, DING Qiulan 2020, 19 (1):  20-27.  DOI: 10.16150/j.1671-2870.2020.01.006 Abstract ( 382 )   HTML ( 1 )   PDF (1261KB) ( 77 )   Objective: To investigate the molecular pathogenesis of 6 pedigrees with inherited coagulation factorⅩ (FⅩ) deficiency and to assess the bleeding risk in patients with inherited FⅩ deficiency by thrombin generation test (TGT).Methods: Clinical data of the 6 pedigrees were collected. The FⅩ coagulation activity (FⅩ:C) was tested by clotting test and FⅩ antigen (FⅩ:Ag) was detected by double antibody sandwich enzyme-linked immunosorbent assay（ELISA）. The concentration of FⅩ in plasma was measured with Western blotting. The FⅩ gene (F10) mutation was analyzed by direct sequencing. Thrombin generation (TG) in six patients and some family members was measured using TET. Results: Ten F10 mutations were identified in 6 probands, five of which (p.Cys 246 Ser, p.Tyr 319 Cys, p.Leu 252 Phe, p.Arg 313 Gln and p. IVS5-2A>G) were novel and the other five(p.Phe71Ser, p.Val 338 Met, p.Gly 406 Ser, p.Gly 365 Ser and p.Cys 151 Arg) had been previously reported. TGT showed that endogenous thrombin potential (ETP) and peak height (Peak) had certain correlation with bleeding severity of FX-deficient patients. Conclusions: Five noval mutations of FⅩ are identified. Five patients were resulted from compound heterozygous F10 mutations, while the remaining one is caused by one heterozygous F10 mutation. TGT parameters including ETP and Peak could be used to assess the bleeding risk in patients with FⅩ deficiency, and TGT might serve as a useful laboratory tool to monitor the risk of bleeding. Figures and Tables | References | Related Articles | Metrics

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Research on correlation between results of diluted Russell viper venom time (dRVVT) test and mixing test in detection of lupus anticoagulant ZHU Chao, GUAN Jialiang, LI Zhe, LIU Yu, WANG Xuefeng, WU Wenman 2020, 19 (1):  28-31.  DOI: 10.16150/j.1671-2870.2020.01.007 Abstract ( 477 )   HTML ( 2 )   PDF (470KB) ( 184 )   Objective: To investigate the correlation between diluted Russell viper venom time(dRVVT) test and mixing test in detection of lupus anticoagulant (LA) and their methodological characteristics. Methods: The clinical data of 31 patients with coagulation dysfunction and positive LA were collected from Ruijin Hospital, Shanghai Jiao Tong University from September 2015 to September 2019. The correlation between the results of standardized ratio of dRVVT and mixing test (Rosner index) was analyzed, and the similarities and differences of the two methods were explored. Results: The scatter diagram revealed that the standardized ratio of dRVVT had a close correlation with the activated partial thromboplastin time（APTT）, R1 and R2. Pearson correlation analysis showed that there was a significant correlation between the standardized ratio of dRVVT and the results of mixing test(r: 0.54-0.62). Conclusions: Although there is a significant correlation between dRVVT based on common pathway and the mixing test based on both intrinsic pathway and common pathway, yet it is not a strict linear correlation. The two methods cannot be completely replaced by each other in the detection of lupus anticoagulant, and it is suggested that the combined use of the two methods can improve the detection rate of lupus anticoagulant and reduce the misdiagnosis and missed diagnosis. Figures and Tables | References | Related Articles | Metrics

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Value of P-wave dispersion, NT-proBNP for prediction of recurrence of atrial fibrillation following cryoballoon ablation LUO Xiaoying, XU Yan, ZHANG Fengru, WU Liqun, QI Wenhang 2020, 19 (1):  32-36.  DOI: 10.16150/j.1671-2870.2020.01.008 Abstract ( 305 )   HTML ( 1 )   PDF (507KB) ( 45 )   Objective: To investigate the predictive value of P-wave dispersion and N-terminal pro-brain natriuretic peptide (NT-proBNP) for recurrence of atrial fibrillation (AF) after successful cryoballoon ablation. Methods: A total of 182 patients with paroxysmal AF with successful cryoballoon ablation were enrolled. A 12-lead electrocardiogram (EKG) was used to record the P-wave for calculating P-wave dispersion before ablation and 1 day, 1 month, 3 months and 6 months after ablation, and plasma NT-proBNP was measured at the same time. A 6-month follow-up was carried out for studying the recurrence of AF, and the clinical, EKG and ablation data were analyzed. Results: The recurrence rate of AF within 6 months after successful cryoballoon ablation was 21.4%. Compared with patients with AF recurrence, those without AF recurrence were younger in age [(47.6±6.1) years vs. (60.2±9.5) years], having higher percentage of lone AF（69.5% vs. 47.3%） and smaller P-wave dispersion. P-wave dispersions were decreased at 3, 6 months after ablation in patients without AF recurrence, while in patients with AF recurrence no significant changes were found. Level of NT-proBNP in patients without AF recurrence were lower than those in patients with AF recurrence. Level of NT-proBNP was decreased after ablation in patients without AF recurrence, while no significant change was found in patients with AF recurrence. Meanwhile patients remained free from AF for 6 months had smaller left atrial diameter(LAD) before ablation than those with recurrence of AF[（41.3±2.7） mm vs.( 46.6±4.4) mm], and LAD was further decreased at 6 months [from(41.3±2.7) mm to (38.0±2.0) mm] (P<0.05). Multivariate regression analysis showed that LAD (OR: 1.3, 95% CI: 0.9-1.0, P<0.05), P-wave dispersion (OR: 1.2, 95% CI: 1.0-1.1, P=0.04） and NT-proBNP level(OR: 2.3, 95%CI: 1.3-1.7, P=0.03) before ablation were independent predictors of AF recurrence after ablation. Conclusions: P-wave dispersion and NT-proBNP level may provide reference information for prediction of recurrence of AF after cryoballon ablation. Figures and Tables | References | Related Articles | Metrics

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Clinical characteristics and misdiagnosis analysis of sparganosis：A retrospective study of 52 cases SHI Dongmei, WANG Xiaolin, CHEN Lu, XIE Qing 2020, 19 (1):  37-43.  DOI: 10.16150/j.1671-2870.2020.01.009 Abstract ( 633 )   HTML ( 3 )   PDF (983KB) ( 182 )   Objective: To analyze the clinical characteristics and causes of misdiagnosis of sparganosis. Methods: A total of 52 cases with sparganosis were enrolled from August, 2006 through August, 2018 in Department of Infectious Diseases of Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. The epidemiological and clinical data were retrieved and causes of misdiagnosis were retrospectively analyzed. Result: The sparganosis was mostly found in middle aged man with an average age of 39.8 years and male to female ratio of 1.36∶1. The sparganosis was highly likely to be misdiagnosed and 57.7% of patients (30/52) had not be recognized upon first clinical consultation. Thirteen patients with the cerebral sparganosis had been misdiagnosed as ascerebral tumor (4/52), brain abscess(2/52), encephalitis granuloma(2/52), and epilepsy(5/52), while patients with extracranial sparganosis(17/52)were considered as pulmonary infection (7/52), pulmonary Tuberculosis(1/52), acute appendicitis(2/52), chronic gastritis combined with twelve fat intestinal ulcer 1/52), intestinal Crohn with obstruction (1/52), liver abscesss (1/52), splenic space-occupying lesion (1/52), rheumatoid arthritis(1/52), chronicmyelocytic leukemia(1 /52). Inquiry of clinical history revealed raw frogs or snakes eating were seen in most of patients(63.5%, 33/52）with eosinophilia as a common laboratory findings (53.8%, 28/52). Serum anti-sparganosis. antibody was detected in all the 52 patients and the diagnosis were all confirmed by pathological examination and identification of worm body. Most cases were cured (84.6%, 44/52), with either praziquantel alone (42.3%, 22/52) or combined with operation (42.3%, 22/52). Eight cases (15.4%) had the relapse. Conclusions: The clinical manifestations of sparganosis varies among patients and is often misdiagnosed. The proper epidemiological investigation is critical for differential diagnosis, particularly for patients with atypical presentation. The MRI imaging study and serum screening of antibody against parasite are essential for establishing the diagnosis. Figures and Tables | References | Related Articles | Metrics

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Effect of Haemophilus influenzae colonizing lower respiratory tract on airway inflammation and its signaling pathway in asthmatic mice KANG Jianqiang, DONG Yangyang, YANG Ling, SONG Zhen, FAN Jiaying 2020, 19 (1):  44-49.  DOI: 10.16150/j.1671-2870.2020.01.010 Abstract ( 261 )   HTML ( 2 )   PDF (958KB) ( 49 )   Objective: To observe the effect of Haemophilus influenzae colonizing lower respiratory tract on airway inflammation in asthmatic mice and study the related signaling pathway. Methods: Thirty-two C57/B6 mice were sensitized and challenged with ovalbumin (OVA) to establish a mice model of chronic asthma (AC group), and then half of them received intratracheal injection of Haemophilus influenza coated in agar beads to built a mice model of chronic asthma with airway Haemophilus influenza colonization (AS group). Besides, 16 C57/B6 mice were treated with intratracheal injection of Haemophilus influenza (NS group) and 16 C57/B6 mice received intratracheal injection of 0.9% NaCl (NC group). Sixty-four TLR4-/- mice were treated and grouped as C57/B6 mice. Mice including C57/B6 and TLR4-/- mice were sacrificed at 7 and 14 days after intratracheal injection, and serum tumor necrosis factor α(TNF-α) content, total number of cells in bronchoalveolar lavage fluid( BALF), and the expression of MyD88 and NF-κB in lung tissue were measured. The indice in AS group were compared with those in the control groups (AC, NS and NC) at corresponding time. Results: The serum level of TNF-α, total number of cells in BALF, and expression of MyD88 and NF-κB in lung tissue were higher in AS group than those in NC and NS group (both in C57/B6 mice and TLR4-/- mice). Compared with C57/B6 wild-type mice, TLR4-/- mice in AS group had lower levels of serum TNF-α, decreased numbers of cells in BALF, and down-regulated expression of MyD88 and NF-κB in lung tissue. Conclusions: Lower airway colonization with Haemophilus influenzae may aggravate airway inflammation of asthma by activating the TLR4-MyD88 pathway and promoting expression of NF-κB, which may be one of the important mechanisms of asthma. Figures and Tables | References | Related Articles | Metrics

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Comparison of live birth rate between frozen-thawed single and double cleavage-stage embryo transfer and single blastocyst transfer in advanced age females and related influencing factors CONG Yanyan, SHEN Xi, KUANG Yanping 2020, 19 (1):  50-54.  DOI: 10.16150/j.1671-2870.2020.01.011 Abstract ( 459 )   HTML ( 1 )   PDF (542KB) ( 80 )   Objective: To analyze the pregnancy outcome and influencing factors related to live birth rate of single cleavage-stage embryos, double cleavage-stage embryos and single blastocyst transfer in elderly patients (40≤age≤50 years) who underwent frozen-thawed embryo transfer. Methods: The elderly patients undergone frozen-thawed embryo transfer from January 2010 to June 2018 were enrolled. The patients were divided into three groups according to the number and stage of transferred embryo: Group D3(Day3, embryo transferred was the third day after fertilization, n=1 363), Group 2D3(two embryos transferred were the third day after fertilization, n=5 248) and Group BT (blastocyst transferred was the fifth or sixth day after fertilization, n=495). Live birth outcome was analyzed and related influencing factors were explored by multivariate Logistic regression. Results: There were significant differences in live birth rates (6.53% vs. 13.38% vs. 18.38%) between the three groups（D3, 2D3, BT）(P<0.01). Twin live birth rate was the highest in 2D3 group (1.12% vs. 7.98% vs. 1.10%)(P<0.05). Multivariate logistic regression analysis revealed that when 2D3 was taken as the reference group, the live birth rate of D3 group was lower (OR=0.558, 95%CI 0.440-0.707, P<0.001), and the live birth rate of BT group had a trend of rising (OR=1.257, 95%CI 0.977-1.617, P=0.075). The live birth rate was negatively correlated with the increase of age（OR=0.685, 95%CI=0.654-0.718） and BMI (OR=0.961, 95%CI=0.935-0.987), and the use of endometrium preparation method (hormone replacement method, with ovulation cycle as reference) might lead to a lower live birth rate(OR=0.736, 95%CI=0.631-0.857). And the live birth rate was positively correlated with thicker endometrium (OR=1.058, 95%CI=1.024-1.094). The live birth rates of patients at 40, 41, 42, 43, 44 and 45 years old were calculated. It was found that the live birth rate was higher in BT group of different ages (P=0.024, 0.01, 0.198, 0.009, 0.102, 0.064), and the live birth rate gradually decreased with the increase of age. Conclusions: Single blastocyst transfer in elderly patients can achieve a similar live birth rate compared to double cleavage-stage embryo transfer, but twin delivery is lower. Figures and Tables | References | Related Articles | Metrics

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Analysis of intestinal microbial composition in specific pathogen free mice using high-throughput sequencing LI Hui, FENG Jie, HAN Lizhong 2020, 19 (1):  55-62.  DOI: 10.16150/j.1671-2870.2020.01.012 Abstract ( 392 )   HTML ( 4 )   PDF (1686KB) ( 72 )   Objective: To analyze the intestinal microbial composition in specific pathogen free(SPF) mice using high-throughput sequencing. Methods: A total of 65 SPF mice of 14 strains were collected. Samples of fresh ileocecus content from the SPF mice were examined using high-throughput sequencing, and 16S rRNA V3-V4 variable regions of bacterium were deeply sequenced. The composition, alpha diversity, and the abundance of microbial flora in ileocecus content were explored, while Staphylococcus, Pseudomonas, Mycoplasma and Corynebacterium were identified at the genus level. Results: Altogether 144 318 high-quality genome sequences were obtained after data filter and quality control. Remarkable differences were found in Alpha diversity (species richness, diversity and evenness) of the microbial composition between samples from mice of the same strains. At the phylum level, the main sequences were largely concentrated as follows: Firmicutes, Bacteroidetes, Proteobacteria, Verrucomicrobia, Actinobacteria, Saccharibacteria, Tenericutes and Spirochaetae. At the genus level, Staphylococcus, Pseudomonas, Anaeroplasma and Corynebacterium were detected in some samples. Conclusions: The composition, alpha diversity and the abundance of the microbial flora in ileocecus contents are substantially different in mice of same strain. There is a need to keep intestinal microbial composition in SPF mice under control, and high-throughput sequencing may offer such possibility. Figures and Tables | References | Related Articles | Metrics

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Clinical and pathological analysis of systemic Epstein-Barr virus positive T-cell lymphoma of childhood:A case report and literature review LI Qinqin, JIN Xiaolong, YUAN Fei 2020, 19 (1):  63-68.  DOI: 10.16150/j.1671-2870.2020.01.013 Abstract ( 745 )   HTML ( 3 )   PDF (1061KB) ( 154 )   Objective: To investigate the clinicopathological characteristics of systemic Epstein-Barr virus (EBV) positive T-cell lymphoma of childhood and improve the understanding of the new diagnostic criteria. Methods: Clinical presentations, pathological characteristics, gene detection results and immunohistochemical phenotyping of biopsy tissue from a case of systemic EBV positive T-cell lymphoma of childhood were analyzed and the relevant literature was reviewed. Result: The 11-year-old boy with prolonged mite allergy treatment and no responsive rash on limbs and torso presented recurrent high fever, generalized lymphadenopathy and hemophagocytic syndrome. Lymph node biopsy revealed diffuse proliferation of small to intermediate neoplastic T cells, which was possibly originated in interfollicular area and positive for CD3（+++）, CD4（+）, TIA-1（+++）, GranzymeB（+++） and MIB-1 (>80% positive). The in situ hybridization of EBV (EBER) was positive （+++） and clonal TCRγ gene rearrangemen was also detected. The diagnosis of systemic EBV positive T-cell lymphoma was thus established. Conclusions: Systemic EBV-positive T-cell lymphoma of childhood is a rare systemic illness characterized by clonal proliferation of EBV-infected T cells and aggressive and fulminant clinical course often associated with hemophagocytic syndrome, which requires differentiation with medical conditions such as invasive NK cells, leukemia/lymphoma, EBV infection associated lymphoproliferative lesions and primary cutaneous gamma-delta T-cell lymphoma. Figures and Tables | References | Related Articles | Metrics

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Serological identification complemented with gene detection for verification of ABO difficult blood groups CHEN Ping, ZHANG Shuimu, LEI Hang, WANG Yujing, CHEN Yu, WANG Xuefeng, CAI Xiaohong 2020, 19 (1):  69-73.  DOI: 10.16150/j.1671-2870.2020.01.014 Abstract ( 355 )   HTML ( 1 )   PDF (649KB) ( 150 )   Objective: To investigate serological identification complemented with gene detection for the verification of difficult ABO blood group. Methods: Seven specimens of difficult ABO blood type cases were serologically stereotyped positively and negatively (saliva blood group substance identification or absorb-diffusion test was added if necessary), and ABO, H, SE gene detection was performed by PCR amplification and direct sequencing. Results: Serological phenotypes of 7 ABO difficult blood group specimens were: 3 cases of para-Bombay blood group, 2 cases of B(A), 2 cases of Bw. Gene detection showed that alleles with H gene mutation were present in all 3 cases of para-Bombay blood group, including 1 case of FUT1* 01N.13/01N.13 and 2 cases of FUT1* 01N.06/01N.06. Three cases of ABO subtype showed 1 case of BA.04/O.01.02, 1 case of BA.04/O.01.01, and 1 case of B3.03/O.01.01. One case of ABO subtype had no ABO gene mutation detected. Conclusions: Gene detection is significant for the identification of para-Bombay blood group and some ABO subtypes, including B(A) subtype, and is a beneficial supplement for serological identification. Figures and Tables | References | Related Articles | Metrics

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Analysis of clinical and laboratory characteristics of EB virus infection in 67 adult patients HU Ying, BAO Yujie, BAI Yupan, LU Guanzhu, CHENG Yajiao, GUO Zhuying, XU Jie 2020, 19 (1):  74-79.  DOI: 10.16150/j.1671-2870.2020.01.015 Abstract ( 841 )   HTML ( 9 )   PDF (597KB) ( 218 )   Objective: To study the clinical characteristics of Epstein-Barr virus(EB virus) infection in adult patients by detecting EB virus-associated antibodies, EB virus DNA, blood routine, blood biochemistry and analyzing clinical symptoms. Methods: A total of 67 adults with EB virus infection were enrolled. Patients were divided into two age groups:young and middle-aged group (18-59 years old, 19 cases) and elderly group (60 years old and above, 48 cases), and 49 healthy subjects including 30 young and middle-aged and 19 elderly people were served as aged group controls. The clinical characteristics of EB virus infection were studied and the differences between the two age groups were compared. Results: Fever was the most common presenting symptom (37 cases, 55.2%), and followed by enlarged lymph nodes(13 cases,19.4%), splenomegaly (9 cases,13.4%), liver enlargement(8 cases, 11.9%), pharyngeal congestion (6 cases, 9.0%), and rash (5 cases, 7.5%). Fever (84.2% vs 43.8%), pharyngeal congestion (26.3% vs 2.1%), rash (21.1% vs 2.1%), liver enlargement (31.6 % vs 4.2%) in young and middle-aged patients were more common than in elderly group ( χ2 values: 9.012, 7.057, 4.612, 7.295, P<0.05). Two cases in young and middle-aged group had malignant tumor diseases (10.5%), while 2 cases (4.1%) in elderly group had hematopoietic lymphohistiocytosis and 17 cases (34.7 %) had malignant tumor diseases. The constituent ratio of cases with malignant tumor diseases between the two groups was significantly different ( χ2=4.15, P=0.042). The total number of T lymphocytes, count of CD4 + T lymphocytes, and B lymphocytes in the elderly group were lower than those in the healthy control group (t=4.525, 5.960, 2.999, P all <0.05), while the number of lymphocyte subsets in the young and middle-aged group was not significantly different from that in the healthy controls(P>0.05). There were no statistical differences in liver function indices and levels of EB virus-related antibodies between young and middle-aged and elderly patients(P>0.05). Conclusions: Clinical manifestations of EB virus infection in adults are varied. The symptoms of acute infection are more common in young and middle-aged patients than in the elderly patients. The cellular immunity and humoral immunity are decreased in elderly patients, and the constituent ratio of cases with EB virus-related tumors is high. Figures and Tables | References | Related Articles | Metrics

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Comparison of different image reconstruction methods in MSCT angiography of occipital artery SUN Furong, CHEN Kemin, PAN Zilai, XU Jingci, RAO Min 2020, 19 (1):  80-83.  DOI: 10.16150/j.1671-2870.2020.01.016 Abstract ( 335 )   HTML ( 4 )   PDF (645KB) ( 54 )   Objective: To assess the performance and clinical significance of head MSCT angiography（MSCTA） in displaying occipital artery（OA）. Methods: The primeval MSCTA imaging data of 80 cases were processed with three reconstruction methods respectively,including shade volume rendering（SVR）, maximal intensity projection（MIP）and curved plannar reconstruction（CPR）, and the diameters of OA at origin and of occipital artery groove at the midpoint were measured. The quality of images derived from the three reconstruction methods were evaluated. Results: The left OA diameter was (2.33±0.26) mm at the origin, and (2.30±0.24) mm at the midpoint of occipital artery groove. The right OA diameter was (2.28±0.24) mm at the origin, and (2.30±0.25) mm at the midpoint of occipital artery groove. The quality of image constructed by SVR and MIP showed no significant difference (t=1.91, P>0.05), however, SVR and MIP yielded image with higher quality than CPR（t=10.70, t=-10.03, P<0.01）. Conclusions: Both SVR and MIP assist visualization of the anatomic structure of OA in head MSCTA. Figures and Tables | References | Related Articles | Metrics

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Performance verification of a full automatic enzyme immunoassay analyzer for the detecting of HAV-IgM antibody WANG Fenglei, WU Xiaoyan, WANG Zhicheng 2020, 19 (1):  84-87.  DOI: 10.16150/j.1671-2870.2020.01.017 Abstract ( 388 )   HTML ( 3 )   PDF (451KB) ( 72 )   Objective: To verify and evaluate the performance of a full automatic enzyme immunoassay analyzer (Tecan) for the detecting of hepatitis A virus(HAV)-IgM. Methods: According to the EP5-A2 document of Clinical and Laboratory Standards Institute（CLSI）and related documents of China National Accreditation Service for Conformity Assessment-CL39（CNAL-CL39） in combination with the condition of our laboratory, the precision, detection limit, accuracy and cut off value of the full automatic enzyme immunoassay analyzer Tecan for the detecting of HAV-IgM were eva-luated.Results: The intra-and inter-batch coefficient of variation (CV) of Tecan for detecting HAV-IgM were 7.77% and 11.17%, respectively, and the coincidence rates of both positive and negative results were 100%. The detection limit was 1.0 NCU/mL, which met the requirement of laboratory and was close to the statement of manufacturer (2.0 NCU/mL). In the validation experiment for cut-off value, the light absorption value ($\bar{x}$+3SD)=0.032 was less than the value 0.105 provided by the kit. Conclusions: The precision, accuracy, detection limit and cutoff value of the automatic enzyme immunoassay analyzer Tecan for detecting HAV-IgM meet the requirements of manufacturer's standards and clinical needs. Figures and Tables | References | Related Articles | Metrics