Serological identification complemented with gene detection for verification of ABO difficult blood groups

doi:10.16150/j.1671-2870.2020.01.014

Journal of Diagnostics Concepts & Practice ›› 2020, Vol. 19 ›› Issue (1): 69-73.doi: 10.16150/j.1671-2870.2020.01.014

• Original articles • Previous Articles Next Articles

Serological identification complemented with gene detection for verification of ABO difficult blood groups

CHEN Ping¹, ZHANG Shuimu¹, LEI Hang², WANG Yujing², CHEN Yu³, WANG Xuefeng², CAI Xiaohong²()

1. Department of Transfusion, Xiamen Hospital, Beijing University of Chinese Medicine(Xiamen Hospital of Traditional Chinese Medicine), Fujian Xiamen 361009, China
2. Department of Clinical Transfusion Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
3. Department of Laboratory, Shanghai Jiading Maternal Child Health Hospital, Shanghai 201821, China

Received:2019-03-20 Online:2020-02-25 Published:2020-02-25
Contact: CAI Xiaohong E-mail:cxh8407@126.com

Abstract

Abstract:

Objective: To investigate serological identification complemented with gene detection for the verification of difficult ABO blood group. Methods: Seven specimens of difficult ABO blood type cases were serologically stereotyped positively and negatively (saliva blood group substance identification or absorb-diffusion test was added if necessary), and ABO, H, SE gene detection was performed by PCR amplification and direct sequencing. Results: Serological phenotypes of 7 ABO difficult blood group specimens were: 3 cases of para-Bombay blood group, 2 cases of B(A), 2 cases of Bw. Gene detection showed that alleles with H gene mutation were present in all 3 cases of para-Bombay blood group, including 1 case of FUT1* 01N.13/01N.13 and 2 cases of FUT1* 01N.06/01N.06. Three cases of ABO subtype showed 1 case of BA.04/O.01.02, 1 case of BA.04/O.01.01, and 1 case of B3.03/O.01.01. One case of ABO subtype had no ABO gene mutation detected. Conclusions: Gene detection is significant for the identification of para-Bombay blood group and some ABO subtypes, including B(A) subtype, and is a beneficial supplement for serological identification.

Key words: ABO subtype, para-Bombay blood group, ABO gene, FUT1 gene

CLC Number:

R5446.11

CHEN Ping, ZHANG Shuimu, LEI Hang, WANG Yujing, CHEN Yu, WANG Xuefeng, CAI Xiaohong. Serological identification complemented with gene detection for verification of ABO difficult blood groups[J]. Journal of Diagnostics Concepts & Practice, 2020, 19(1): 69-73.

Figures/Tables 3

References 19

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标本	正定型			反定型				唾液血型物质		Lewis血型
标本	抗-A	抗-B	抗-H	A_c	B_c	O_c	自身c	A_c	B_c	抗-L e^a	抗-L e^b
1	-	-	-	3+	-	-	-	-	+	-	+
2	-	-	-	-	3+	-	-	+	-	-	+
3	-	-	-	4+	4+	2+	-	-	-	-	+
4	1+	4+	3+	4+	-	-	-	/	/	/	/
5	2+	4+	4+	2+	-	-	-	/	/	/	/
6	-	2+	3+	4+	-	-	-	-	+	/	/
7	-	2+	3+	3+	-	-	-	-	+	/	/

标本	血清学表型	相应的基因分型	关键核苷酸突变
1	B类孟买	FUT101N.13/01N.13*	FUT1*c.881_882de1TT
2	A类孟买	FUT101N.06/01N.06*	FUT1*c.551_552delAG
3	O类孟买	FUT101N.06/01N.06*	FUT1*c.551_552delAG
4	B(A)	BA.04/O.01.02	ABO*c.640A>G
5	B(A)	BA.04/O.01.01	ABO*c.640A>G
6	Bw	B3.03/O.01.01	ABO*c.155+5G>A

Serological identification complemented with gene detection for verification of ABO difficult blood groups

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