Abstract:

Objective: To explorethe value of megakaryocyte immunolabeling performed on bone marrow biopsy section embedded in glycol-methacrylate (GMA) plastic in the diagnosis of myelodysplastic syndrome (MDS) with thrombocytopenia. Methods: Bone marrow biopsy samples acquired from 47 patients with thrombocytopenia were processed with glycol-methacrylate (GMA) embedding technique, and megakaryocyte immunolabeling were performed. Results: Bone marrow biopsy plastic embedded technology improved themorphologic diagnosis of MDS, and megakaryocyte immunolabeling enabled identification of the small or micor sized megakaryocyte. Out of the cases examined, 8 were diagnosed as myelodysplastic syndrome (MDS), including 3 cases of myelodysplastic syndrome-single lineage dysplasia (MDS-SLD), 1 case of 5q- syndrome and 4 cases of myelodysplastic syndrome-multilineage dysplasia (MDS-MLD). All of the 8 cases were positive for megakaryocyte markers, and 6 cases of them showed clear abnormal localization of immature precursor (ALIP) in the section, and 3 cases had chromosomal abnormalities detected. Conclusion: Combination of GMA plastic embedded technology and megakaryocyte immunolabeling facilitate the diagnosis of MDS with thrombocytopenia.

Key words: Bone marrow biopsy, Plastic embedding, Myelodysplastic syndrome, Thrombocytopenia