The abnormal percentage of bone marrow macrophages and its clinical significances in myelodysplastic syndromes

doi:10.16150/j.1671-2870.2018.04.016

Abstract

Abstract: Objective: To investigate the percentage of bone marrow macrophages and its correlations with IPSS (international prognostic scoring system) score and percentage of bone marrow CD34⁺ cells in patients with myelodysplastic syndromes (MDS). Methods: The percentages of bone marrow macrophages were detected by flow cytometry using CD14 and CD68 labeling and the activation of Th cells was detected by flow cytometry using CD3, CD8, IFN-γ, IL-4 labeling. Results: ① Low-risk MDS demonstrated increased percentage of macrophages (1.56%±0.22%) in bone marrow compared to patients with high-risk MDS (0.20%±0.07%) (P<0.001) and controls (0.62%±0.09%) (P<0.001). ② The percentage of bone marrow macrophages was correlated positively with the proportion of Th1 cells (r=0.434, P<0.01). ③ The percentage of bone marrow macrophages was correlated negatively with IPSS score (r=-0.532, P<0.001), percentage of bone marrow myeloblast cells (r=-0.457, P<0.01), peripheral blood hemoglobin concentration (r=-0.398, P<0.01) and percentage of bone marrow CD34⁺ cells (r=-0.324, P<0.05). Conclusions: The increased percentage of bone marrow macrophages may contribute to the abnormal activation of Th1 cells in low-risk MDS. Detecting the percentage of macrophage may be helpful in evaluating the prognosis of MDS.

Key words: Myelodysplastic syndromes, Macrophages, Prognosis

CLC Number:

R733.3

SONG Dandan, CHANG Chunkang, GUO Juan, XU Feng, ZHAO Youshan, WU Lingyun. The abnormal percentage of bone marrow macrophages and its clinical significances in myelodysplastic syndromes[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(04): 439-443.

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