Study on drug resistance of carbapenem-resistant Acinetobacter baumannii in a tertiary hospital in Xinjiang from 2023 to 2024

doi:10.16150/j.1671-2870.2025.06.007

Abstract

Abstract:

Objective To analyze the molecular epidemiological characteristics of carbapenem-resistant Acinetobacter baumannii (CRABA) at Karamay Central Hospital from 2023 to 2024, and to investigate the carriage of carbape-nemase and RND efflux pump genes in CRABA, so as to provide an experimental basis for the clinical prevention, control, and treatment of Acinetobacter baumannii infection in the Xinjiang region. Methods CRABA strains isolated from clinical culture at Karamay Central Hospital from 2023 to 2024 were collected, and percentage of CRABA detected in Acinetobacter baumannii (detection rate) was calculated. PCR was used to detect 10 common carbapenemase genes and RND efflux pump genes. Results A total of 179 CRABA strains were obtained with a detection rate of 79.56%. The majority were detected in the ICU (62.60%) and the respiratory intensive care unit (11.73%). The strains showed high resistance rate to antibiotics, with the highest resistance rate observed for ciprofloxacin (100%), and the lowest for tigecycline (11.73%). The detection of drug resistance genes revealed that among the 179 CRABA strains, the positive carriage rates from high to low were: blaIMP 95.53% (171/179), blaOXA-51 87.71% (157/179), blaTEM 82.12% (147/179), adeB 82.12% (147/179), adeM 80.45% (144/179), adeJ 77.65% (139/179), blaOXA-23 74.86% (134/179), blaNDM-1 8.94% (16/179), blaOXA-58 4.47% (8/179), and blaVIM 0 (0/179). Conclusions The detection rate of CRABA at our hospital from 2023 to 2024 were significantly higher than the national average (2023, 73.4%). The high expression of the carbapenemase genes include blaIMP and blaTEM, and RND efflux pump genes adeB, adeM, and adeJ. This suggests that local clinical practice should strengthen the management of antibiotics and ensure their rational use.

Key words: Carbapenem-resistant Acinetobacter baumannii, Carbapenemase, RND efflux pump genes

CLC Number:

R372
R451

JIA Liying, XU Haifeng, YU Chong, GUO Wanhai, LONG Rui. Study on drug resistance of carbapenem-resistant Acinetobacter baumannii in a tertiary hospital in Xinjiang from 2023 to 2024[J]. Journal of Diagnostics Concepts & Practice, 2025, 24(06): 621-627.

Figures/Tables 6

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References 30

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Gene name	Primer sequences(5´→3´)	Length(bp)
Carbapenemase gene
blaTEM	F：ATCAGCAATAAACCAGC	522
blaTEM	R：CCCCGAAGAACGTTTTC	522
blaNDM-1	F：CTCGCACCGAATGTCTGG	713
blaNDM-1	R：CATTGGCGGCGAAAGTCA	713
blaVIM	F：GTTTGGTCGCATATCGCAAC	278
blaVIM	R：AATGCGCAGCACCAGGATAG	278
blaIMP	F：CATGGTTTGGTGGTTCTTGT	524
blaIMP	R：AATATTTTGGCGGACTTTGGC	524
blaOXA-23	F：GATCGGATTGGAGAACCAGA	517
blaOXA-23	R：ATTTCTGACCGCATTTCCAT	517
blaOXA-51	F：TAATGCTTTGATCGGCCTGG	367
blaOXA-51	R：TGGATTGCACTTCATCTTGG	367
blaOXA-58	F：AAGTATTGGGGCTTGTGCTG	603
blaOXA-58	R：CCCCTCTGCGCTCTACATAC	603
RND efflux pump gene
adeB	F：TGGCGGAATGAAGTATGT	885
adeB	R：GCAGTGCGGCAGGTTAG	885
adeM	F：GTAGGTGTAGGCTTATGGA	687
adeM	R：GTACCGAAGTGACTGAAAT	687
adeJ	F：TTAACGATAGCGTTGTAACC	481
adeJ	R：TGAGCAGACAATGGAATAGT	481

Year	Pathogenic bacteria (N)	Acinetobacter baumannii (n)	CRABA(n)	Percentage of CRABA accounting for Acinetobacter baumannii （%）
2023	2 272	101	81	80.20
2024	3 652	124	98	79.03
Total	5 924	225	179	79.56

Specimen source	Number	Percentage（%）
Sputum	146	81.56
Throat swab	10	5.59
Bronchial aspirate	7	3.91
Blood	6	3.35
Secretion	5	2.79
Urine	2	1.12
Bile	2	1.12
Pus	1	0.56
Total	179	100

Specimen source	Number	Percentage（%）
Intensive care unit	112	62.60
Respiratory intensive care unit	21	11.73
Neurological intensive care unit	16	8.94
Respiratory department	12	6.70
Neurology	3	1.68
Neurosurgery	2	1.12
Emergency Department	2	1.12
General foreign language one	2	1.12
Urology	2	1.12
Burn department	1	0.56
Coronar/Care unit	1	0.56
Infection department	1	0.56
Nephrology department	1	0.56
Gastroenterology department	1	0.56
Rehabilitation department	1	0.56
Orthopaedics	1	0.56
Total	179	100

Antibiotic name	Number	Percentage（%）
Compound sulfamethoxazole	46	25.70
Ceftazidime	177	98.88
Cefepime	175	97.77
Piperacillin tazobactam	177	98.88
Cefoperazone and sulbactam	109	60.89
Imipenem	179	100
Meropenem	179	100
Doxycycline	91	50.84
Minocycline	97	54.19
Tobramycin	126	70.39
Levofloxacin	136	75.98
Ciprofloxacin	179	100
Tigecycline	21	11.73