Objective: To screen irregular blood group antibodies and detecte its specificity in pregnant women for exploring the presence of irregular antibodies and related clinical characteristics. Method: Microcolumn gel card was used to screen irregular blood group antibodies in pregnant women undergoing maternal examination in our hospital from January 2017 to August 2018 and analyzing the antibody specificity of positive screening specimens. Results: Of the 7 381 pregnant women, 32 had irregular blood group antibodies detected,the positive rate was 0.434%. Among them, 10 cases were anti-M, 3 cases were anti-E, 8 cases were anti-Ec, 4 cases were anti-D, 4 cases were anti-Lea, 1 case was anti-Dia, 1 case was anti-Wra,1 case was cold antibody. The pregnant women who had irregular blood group antibodies detected all had more than one pregnancy history. Conclusions: The irregular blood group antibodies detection rate was 0.434% in pregnant women taking maternal examination at our hospital; anti-M was the most frequent. The antibodies of Rh blood group system and MNS blood group system could lead to the occurrence of hemolysis disease in newborn infants.