Objective: To assess the value of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesions for providing a basis for its clinical application. Methods: Two hundred cases of cervical intraepithelial lesion selec-ted from January 2015 to June 2016 were served as the object of this study. The biopsy paraffin sections after appropriate antigen retrieval were used for detection of p16 and Ki-67 immunohistochemically and the value of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesion was analyzed. Results: The positive expression rates of p16 and Ki-67 in cervical squamous intraepithelial lesion (SIL) were significantly higher than those in atrophic cervix and cervical squamous epithelial metaplasia (P< 0.05). The positive expression rates of p16 and Ki-67 in high grade squamous intraepithelial lesion (HSIL) were significantly higher than those in low grade squamous intraepithelial lesion (LSIL) (P< 0.05). In cervical intraepithelial lesion, the expressions of p16 and Ki-67 were in good consistency, and was statistically significant (P< 0.05). The p16, Ki67 positive cells extended to 2/3 layer or whole layer in HSIL, and the positive expression rate was significantly higher than that in LSIL (P< 0.05). Conclusions: Detection of p16 and Ki-67 can be used as an important indicator for cervical intraepithelial lesion and has high diagnostic value clinically.
LIU Jingwen, WU Qiu-liang
. Significance of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesions[J]. Journal of Diagnostics Concepts & Practice, 2017
, 16(02)
: 195
-198
.
DOI: 10.16150/j.1671-2870.2017.02.014
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