Significance of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesions

doi:10.16150/j.1671-2870.2017.02.014

Abstract

Abstract: Objective: To assess the value of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesions for providing a basis for its clinical application. Methods: Two hundred cases of cervical intraepithelial lesion selec-ted from January 2015 to June 2016 were served as the object of this study. The biopsy paraffin sections after appropriate antigen retrieval were used for detection of p16 and Ki-67 immunohistochemically and the value of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesion was analyzed. Results: The positive expression rates of p16 and Ki-67 in cervical squamous intraepithelial lesion (SIL) were significantly higher than those in atrophic cervix and cervical squamous epithelial metaplasia (P< 0.05). The positive expression rates of p16 and Ki-67 in high grade squamous intraepithelial lesion (HSIL) were significantly higher than those in low grade squamous intraepithelial lesion (LSIL) (P< 0.05). In cervical intraepithelial lesion, the expressions of p16 and Ki-67 were in good consistency, and was statistically significant (P< 0.05). The p16, Ki67 positive cells extended to 2/3 layer or whole layer in HSIL, and the positive expression rate was significantly higher than that in LSIL (P< 0.05). Conclusions: Detection of p16 and Ki-67 can be used as an important indicator for cervical intraepithelial lesion and has high diagnostic value clinically.

Key words: p16, Ki-67, Cervical intraepithelial lesion, Clinical diagnosis

CLC Number:

R737.3

LIU Jingwen, WU Qiu-liang. Significance of detection of p16 and Ki-67 in diagnosis of cervical intraepithelial lesions[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(02): 195-198.

References

[1] Miccò M, Vargas HA, Burger IA, et al.Combined pre-treatment MRI and ¹⁸F-FDG PET/CT parameters as prognostic biomarkers in patients with cervical cancer[J]. Eur J Radiol,2014,83(7):1169-1176.
[2] Hariri Tabrizi S, Aghamiri SM, Farzaneh F, et al.The use of optical spectroscopy for in vivo detection of cervical pre-cancer[J]. Lasers Med Sci,2014,29(2):831-845.
[3] 汪勤, 解正新, 张卫琴. p16、p53和Ki-67蛋白在宫颈上皮内瘤变中的表达及意义[J]. 临床与实验病理学杂志,2013,29(5):551-553.
[4] Fujii T, Saito M, Hasegawa T, et al.Performance of p16INK4a/Ki-67 immunocytochemistry for identifying CIN2+ in atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion specimens: a Japanese Gynecologic Oncology Group study[J]. Int J Clin Oncol,2015,20(1):134-142.
[5] Kalaivani R, Masilamani V, AlSalhi MS, et al. Cervical cancer detection by time-resolved spectra of blood components[J]. J Biomed Opt,2014,19(5):057011.
[6] 赫芳芳, 高英, 魏力. p16、Ki-67在宫颈鳞状上皮病变分级诊断中的意义[J]. 现代肿瘤医学,2016,24(8):1271-1274.
[7] Yoon W, Choi JH, Kim S, et al.Engineered Salmonella typhimurium expressing E7 fusion protein, derived from human papillomavirus, inhibits tumor growth in cervical tumor-bearing mice[J]. Biotechnol Lett,2014,36(2):349-356.
[8] Torheim T, Malinen E, Kvaal K, et al.Classification of dynamic contrast enhanced MR images of cervical cancers using texture analysis and support vector machines[J]. IEEE Trans Med Imaging,2014,33(8):1648-1656.
[9] 肖兰. p16、Ki-67、MMP-2在宫颈疾病中的应用研究进展[J]. 医学综述,2014,20(18):3285-3286.
[10] Wentzensen N, Schwartz L, Zuna RE, et al.Performance of p16/Ki-67 immunostaining to detect cervical cancer precursors in a colposcopy referral population[J]. Clin Cancer Res,2012,18(15):4154-4162.
[11] 梁晓燕, 姚宏, 张王亮, 等. P16Ki-67免疫组织化学半定量评分在宫颈上皮内瘤变诊断中的意义[J]. 山西医药杂志,2016,45(6):705-709.
[12] 周洪园, 邹燕丽, 卓俊菊, 等. stathmin、p16及Ki-67检测在子宫颈疑难病例中的应用价值[J]. 肿瘤研究与临床,2016,28(7):459-463.
[13] Pacchiarotti A, Ferrari F, Bellardini P, et al.Prognostic value of p16-INK4A protein in women with negative or CIN1 histology result: a follow-up study[J]. Int J Cancer,2014,134(4):897-904.
[14] 王昱, 邓洋, 李卿. 高危型人乳头瘤病毒联合p16、Ki-67检测对子宫颈上皮内瘤变的诊断价值[J]. 肿瘤研究与临床,2015,27(12):823-826.
[15] Alshenawy HA.Evaluation of p16, human papillomavirus capsid protein L1 and Ki-67 in cervical intraepithelial lesions: potential utility in diagnosis and prognosis[J]. Pathol Res Pract,2014,210(12):916-921.