Journal of Diagnostics Concepts & Practice >
The impact of different diluting media on determination of serum estradiol concentration by using chemiluminescent immunoassay
Received date: 2018-12-20
Online published: 2019-08-25
Objective: To evaluate the impact of different diluting media on determination of serum estradiol (E2) concentration by using chemiluminescent immunoassay. Methods: Fifty serum samples were collected for E2 detection using chemiluminescent immunoassay by an automatic biochemical analyzer (UniCel DXI 800, Beckman Coulter). Four diluting media were used, including standard diluent S0 (Beckman Coulter), pooled low-level E2 serum (PS), E2/progesterone standard diluent (Roche), and calf serum (CS). Samples were manually diluted into 1∶1 or 1∶4 using the above 4 media with two parallel tubes and detected by UniCel DXI 800. Multiple statistical methods were applied to analyze the data. Results: Students't test revealed that the E2 concentration measured by diluting media CS, Roche and PS compared with S0 showed significant differences statistically (P=5.03×10-11, 1.44×10-10 and 2.79×10-13, respectively). Pearson correlation analysis showed that the correlation coefficient r between diluting media S0 and CS, Roche, PS were 0.992 3, 0.971 3, 0.968 0, respectively. After correction with linear regression model, the concentration measured by diluting media CS, Roche and PS compared with S0 showed no significant difference statistically (P=0.998 3, 0.997 9, 0.987 7, respectively). Consistence of E2 concentration measured by diluting media CS, Roche and PS compared with S0 was evaluated by Bland-Altman method, the results showed that 2%, 2% and 8% of the samples exceeded the consistence limit, respectively. The maximum bias of three assessed diluting media were 16.8%, 44.8% and 45.2%, respectively. CS shows the best consistence. Conclusions: Among the three assessed diluting media, CS shows the best performance and could serve as a replacement of manufacturer's standard diluent with high stability and lower cost.
Key words: Estradiol; Diluent; Chemiluminescent immunoassay; Bland-Altman method
SI Chenchen, XU Bufang, FAN Weimin, ZHANG Aijun . The impact of different diluting media on determination of serum estradiol concentration by using chemiluminescent immunoassay[J]. Journal of Diagnostics Concepts & Practice, 2019 , 18(04) : 448 -453 . DOI: 10.16150/j.1671-2870.2019.04.013
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