Objective: To investigate the percentage of bone marrow macrophages and its correlations with IPSS (international prognostic scoring system) score and percentage of bone marrow CD34+ cells in patients with myelodysplastic syndromes (MDS). Methods: The percentages of bone marrow macrophages were detected by flow cytometry using CD14 and CD68 labeling and the activation of Th cells was detected by flow cytometry using CD3, CD8, IFN-γ, IL-4 labeling. Results: ① Low-risk MDS demonstrated increased percentage of macrophages (1.56%±0.22%) in bone marrow compared to patients with high-risk MDS (0.20%±0.07%) (P<0.001) and controls (0.62%±0.09%) (P<0.001). ② The percentage of bone marrow macrophages was correlated positively with the proportion of Th1 cells (r=0.434, P<0.01). ③ The percentage of bone marrow macrophages was correlated negatively with IPSS score (r=-0.532, P<0.001), percentage of bone marrow myeloblast cells (r=-0.457, P<0.01), peripheral blood hemoglobin concentration (r=-0.398, P<0.01) and percentage of bone marrow CD34+ cells (r=-0.324, P<0.05). Conclusions: The increased percentage of bone marrow macrophages may contribute to the abnormal activation of Th1 cells in low-risk MDS. Detecting the percentage of macrophage may be helpful in evaluating the prognosis of MDS.
SONG Dandan, CHANG Chunkang, GUO Juan, XU Feng, ZHAO Youshan, WU Lingyun
. The abnormal percentage of bone marrow macrophages and its clinical significances in myelodysplastic syndromes[J]. Journal of Diagnostics Concepts & Practice, 2018
, 17(04)
: 439
-443
.
DOI: 10.16150/j.1671-2870.2018.04.016
[1] Glenthøj A, Ørskov AD, Hansen JW, et al. Immune Mechanisms in Myelodysplastic Syndrome[J]. Int J Mol Sci,2016,17(6),pii:E944.
[2] Gañán-Gómez I, Wei Y, Starczynowski DT, et al.Deregu-lation of innate immune and inflammatory signaling in myelodysplastic syndromes[J]. Leukemia,2015,29(7):1458-1469.
[3] 李晓, 应韶旭, 刘薏芝, 等. 骨髓增生异常综合征巨噬细胞增生与造血细胞凋亡的相关性[J]. 中华病理学杂志,2003,32(3):226-229.
[4] Kitagawa M, Kamiyama R, Kasuga T.Increase in number of bone marrow macrophages in patients with myelodysplastic syndromes[J]. Eur J Haematol,1993,51(1):56-58.
[5] 吴凌云, 李晓, 常春康, 等. 骨髓增生异常综合征患者T细胞异常极化导致的负性造血调控[J]. 中华血液学杂志,2007,28(8):549-554.
[6] Allavena P, Sica A, Garlanda C, et al.The Yin-Yang of tumor-associated macrophages in neoplastic progression and immune surveillance[J]. Immunol Rev,2008,222:155-161.
[7] Mantovani A, Sozzani S, Locati M, et al.Macrophage polarization: tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes[J]. Trends Immunol,2002,23(11):549-555.
[8] Komohara Y, Hasita H, Ohnishi K, et al.Macrophage infiltration and its prognostic relevance in clear cell renal cell carcinoma[J]. Cancer Sci,2011,102(7):1424-1431.
[9] Kamper P, Bendix K, Hamilton-Dutoit S, et al.Tumor-infiltrating macrophages correlate with adverse prognosis and Epstein-Barr virus status in classical Hodgkin's lymphoma[J]. Haematologica,2011,96(2):269-276.
[10] Itoh M, Yago K, Shimada H, et al.Reversible acceleration of disease progression following cyclosporin A treatment in a patient with myelodysplastic syndrome[J]. Int J Hematol,2002,75(3):302-304.
[11] Ohmori S, Ohmori M, Yamagishi M, et al.MDS-macrophage derived inhibitory activity on myelopoiesis of MDS abnormal clones[J]. Br J Haematol,1993,83(3):388-391.
[12] Hom J, Dulmovits BM, Mohandas N, et al.The erythroblastic island as an emerging paradigm in the anemia of inflammation[J]. Immunol Res,2015,63(1-3):75-89.
[13] Reza S, Dar S, Andric T, et al.Biologic characteristics of 164 patients with myelodysplastic syndromes[J]. Leuk Lymphoma,1999,33(3-4):281-287.
[14] Motomura S, Motoji T, Okutomi K, et al.Successful treatment of refractory anemia by high-dose methylprednisolone associated with an increment in CD68-positive cells in bone marrow[J]. Am J Hematol,2001,66(2):80-84.
