Journal of Diagnostics Concepts & Practice >

2019 , Vol. 18 >Issue 03: 263 - 270

DOI: https://doi.org/10.16150/j.1671-2870.2019.03.005

Original articles

Analysis of structural characteristics of gut microbiome in colitis mice based on 16S rRNA high-throughput sequencing

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  • Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China

Received date: 2019-02-21

  Online published: 2019-06-25

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Abstract

Objective: To clarify the interrelationship between ulcerative colitis (UC ) and gut microbiome for providing an experimental basis to find a simple and safe treatment for patients with ulcerative colitis. Methods: A model of ulcerative colitis in mice was established by 3% DSS (dextran sulfate sodium), and the body weight and intestinal pathological changes of the model mice were detected. The diversity and differences of gut microbiome in feces of ulcerative colitis mice and normal mice were identified by 16S rRNA high-throughput sequencing technology. The existing gene database was used to estimate the functional gene composition of the intestinal flora and the functional differences between the two groups. Results: The results of comparative analysis showed that the weight of ulcerative colitis mice was significantly reduced, the integrity of intestinal epithelium was destroyed, and the diversity of gut microbiome was significantly reduced. Bifidobacterium in intestinal tract of ulcerative colitis mice decreased to 0.2%, which was significantly lower than that of mice in control group (P<0.05); and the relative abundance of Lactobacillus also decreased to 2.9% (P<0.05), which denoted that ulcerative colitis might be caused by its influence on metabolism of the body. Conclusions: The diversity and distribution of gut microbiome in ulcerative colitis mice have changed significantly.

Key words： Inflammatory bowel disease; Gut microbiome; 16S rRNA high-throughput sequencing

Cite this article

WANG Tingting, ZHENG Naisheng, YUAN Xiangliang, SHEN Lisong . Analysis of structural characteristics of gut microbiome in colitis mice based on 16S rRNA high-throughput sequencing[J]. Journal of Diagnostics Concepts & Practice, 2019 , 18(03) : 263 -270 . DOI: 10.16150/j.1671-2870.2019.03.005

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