Objective: To analyze the distribution of human papilloma virus (HPV) infection and subtypes in Songjiang District, Shanghai for providing a theoretical basis for prevention of HPV infection. Method: A total of 52 973 gynecological outpatients tested for HPV subtypes by gene chip detection were enrolled in this study. Result: A total of 12 139 patients were infected with HPV. The HBV infection rate was 22.92%. Altogether 16 637 strains of human papilloma virus were detected, and high-risk HPV subtypes accounted for 78.61%. The top five infection subtypes were: HPV16, 58, 52, 51, 68, all of which were high-risk subtypes. Of the infected patients, 8 933 cases were single infection (73.50%), mainly with high-risk subtypes, accounting for 80.48%; 3 206 cases were multiple infection (26.50%), most of which were double infection with high-risk subtype (57.71%), and the common ones were HPV16 and 58. The infection rates of ≤20 years old, 21-30 years old, 31-40 years old, 41-50 years old, 51-60 years old, ≥61 years old groups were 31.83%, 21.48%, 20.66%, 23.69%, 30.35%, 33.88%, respectively. Conclusions: In Songjiang District, HPV infection rate among gynecological outpatients was 22.92%. The most common HPV subtypes were 16, 58, 52, 51, 68. The majority is single infection, and most of which with high-risk subtypes. Among double infection, most were high-risk subtype combination. The age group ≥60 years shows higher infection rate.