Journal of Diagnostics Concepts & Practice >
Serological identification complemented with gene detection for verification of ABO difficult blood groups
Received date: 2019-03-20
Online published: 2020-02-25
Objective: To investigate serological identification complemented with gene detection for the verification of difficult ABO blood group. Methods: Seven specimens of difficult ABO blood type cases were serologically stereotyped positively and negatively (saliva blood group substance identification or absorb-diffusion test was added if necessary), and ABO, H, SE gene detection was performed by PCR amplification and direct sequencing. Results: Serological phenotypes of 7 ABO difficult blood group specimens were: 3 cases of para-Bombay blood group, 2 cases of B(A), 2 cases of Bw. Gene detection showed that alleles with H gene mutation were present in all 3 cases of para-Bombay blood group, including 1 case of FUT1* 01N.13/01N.13 and 2 cases of FUT1* 01N.06/01N.06. Three cases of ABO subtype showed 1 case of BA.04/O.01.02, 1 case of BA.04/O.01.01, and 1 case of B3.03/O.01.01. One case of ABO subtype had no ABO gene mutation detected. Conclusions: Gene detection is significant for the identification of para-Bombay blood group and some ABO subtypes, including B(A) subtype, and is a beneficial supplement for serological identification.
Key words： ABO subtype; para-Bombay blood group; ABO gene; FUT1 gene
CHEN Ping, ZHANG Shuimu, LEI Hang, WANG Yujing, CHEN Yu, WANG Xuefeng, CAI Xiaohong . Serological identification complemented with gene detection for verification of ABO difficult blood groups[J]. Journal of Diagnostics Concepts & Practice, 2020 , 19(1) : 69 -73 . DOI: 10.16150/j.1671-2870.2020.01.014
| [1] | 侯瑞琴, 杨洪燕, 苗天红, 等. 422例ABO正反定型不符的原因分析及处理对策[J]. 中国输血杂志, 2016(5):509-511. |
| [2] | 章旭, 李剑平. 455例ABO正反定型不符原因分析[J]. 中国输血杂志, 2010,23(增刊):133. |
| [3] | 王钰菁, 蔡晓红, 龚淞颂, 等. ABO血型正反不符原因分析及输血策略[J]. 诊断学理论与实践, 2017, 16(3):297-300. |
| [4] | 尚红, 王毓三, 申子瑜, 等. 全国临床检验操作规程[M].4版. 北京: 人民卫生出版社, 2015:118-134. |
| [5] | Cai XH, Jin S, Liu X, et al. Molecular genetic analysis for the B subgroup revealing two novel alleles in the ABO gene[J]. Transfusion, 2008, 48(11):2442-2447. |
| [6] | Yip SP, Chee KY, Chan PY, et al. Molecular geneticanalysis of para-Bombay phenotypes in Chinese:a novel non-functional FUTIallele is identified[J]. Vox Sang, 2002, 83(3):258-262. |
| [7] | 蔡晓红, 林琳, 陆晔玲, 等. FUT1基因突变导致类孟买血型的分子机制[J]. 中华检验医学杂志, 2015, 38(11):765-767. |
| [8] | 向东. ABO亚型的检测[J]. 中国输血杂志, 2010, 23(8):577-579. |
| [9] | 赵桐茂. 基因分型预测ABO亚型的局限性[J]. 临床输血与检验, 2018, 20(2):113-116. |
| [10] | Yu LC, Yang YH, Broadberry RE, et al. Heterogeneity of the human H blood group alpha(1,2) fucosyltransferase gene among para-Bombay individuals[J]. VOX Sang, 1997, 72(1):36-40. |
| [11] | Yan L, Zhu F, Xu X, et al. Molecular basis for para-Bombay phenotypes in Chinese persons including a novel nonfunctional FUT1allele[J]. Transfusion, 2005, 45(5):725-730. |
| [12] | Cai XH, Jin S, Liu X, et al. Molecular genetic analysis for the para-Bombay blood group revealing two novel alleles in the FUT1 gene[J]. Blood Transfus, 2011, 9(4):466-468. |
| [13] | 金沙, 蔡晓红, 刘曦, 等. 上海地区献血人群cisAB和B(A)血型的研究[J]. 中国输血杂志杂志, 2013, 26(12):1198-1201. |
| [14] | 崔文燕, 王钰箐, 邹纬, 等.α1, 3-D-半乳糖基转移酶基因425C→T突变导致B3亚型分析[J]. 郑州大学学报(医学版), 2018, 53(5):655-658. |
| [15] | 李归冀, 章旭, 等. ABO变异型B305血型基因亚型的鉴定及序列分析[J]. 中国实验血液学杂志, 2016, 24(5):1563-1566. |
| [16] | Cai XH, Jin S, Liu X, et al. Molecular genetic analysis of ABO blood group variations reveals 29 novel ABO subgroup alleles[J]. Transfusion, 2013, 53(11Suppl2):2910-2916. |
| [17] | Cai XH, Fang CJ, Jin S, et al. H blood-group deficiency has a high frequency in Lahu Chinese[J]. Transfus Med, 2011, 21(3):209-10. |
| [18] | Huang H, Jin S, Liu X, et al. Molecular genetic analysis of weak ABO subgroups in the Chinese population reveals ten novel ABO subgroup alleles[J]. Blood Transfus, 2018, 3:1-6. |
| [19] | 王显荣, 许立, 李育, 等. 盐水法结合柱凝集法在ABO疑难血型鉴定中的应用[J]. 中国输血杂志, 2006, 19(1):34-36. |
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