Journal of Diagnostics Concepts & Practice >

2018 , Vol. 17 >Issue 06: 640 - 644

DOI: https://doi.org/10.16150/j.1671-2870.2018.06.004

Original articles

Plasmids typing of genes carrying blaKPC-2 in Klebsiella pneumoniae isolated at a tertiary hospital in Shanghai

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  • 1. Department of Laboratory Medicine, Shanghai First Maternity and Infant Hospital, Tongji University, Shanghai 200040, China;
    2. Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China

Received date: 2018-09-05

  Online published: 2018-12-25

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Abstract

Objective: To investigate the plasmids typing of genes carrying blaKPC-2 in carbapenemase resistant Klebsiella pneumoniae for exploring its epidemiological evolution. Methods: Thirty-eight carbapenem -resistant Klebsiella pneumoniae isolates obtained consecutively from Huashan Hospital were collected, all carrying blaKPC-2-bearing plasmids. All the strains were subjected to multilocus sequence typing (MLST). Relaxase analysis was used to classify these plasmids. Results: Eight of the blaKPC-2-bearing plasmids belonged to the MOBP3 and 30 blaKPC-2-bearing plasmids belonged to the MOBF12. Phylogenetic analysis revealed that the MOBF12 plasmids were located at two different branches, respectively, suggesting they had different biological origin. Conclusions: It is the first time that relaxase has been used for plasmids typing of genes carrying blaKPC-2 in carbapenemase resistant Klebsiella pneumoniae, and found 8 belonged to the MOBP3 and 30 belonged to the MOBF12, and the MOBF12 having different biological origin, denoting the epidemiological evolution of blaKPC-2 bearing plasmid in carbapenemase resistant Klebsiella pneumoniae.

Key words： Klebsiella pneumoniae; blaKPC; Plasmid; Relaxase

Cite this article

SHEN Pinghua, ZHANG Xianhua, JIANG Xiaofei, CHEN Huifen . Plasmids typing of genes carrying blaKPC-2 in Klebsiella pneumoniae isolated at a tertiary hospital in Shanghai[J]. Journal of Diagnostics Concepts & Practice, 2018 , 17(06) : 640 -644 . DOI: 10.16150/j.1671-2870.2018.06.004

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