Journal of Diagnostics Concepts & Practice >

2022 , Vol. 21 >Issue 04: 514 - 519

DOI: https://doi.org/10.16150/j.1671-2870.2022.04.016

Original articles

Application of long non-coding RNA ENST00000489676 detection in ultrasonographic evaluation of cervical lymph node metastasis in papillary thyroid carcinoma

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  • Department of Ultrasound, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received date: 2021-03-20

  Online published: 2022-11-07

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Abstract

Objective: To analyze the value of lncRNA ENST0000048967 detection in biopsy tissue of papillary thyroid carcinoma (PTC) by ultrasound-guided fine-needle aspiration (US-FNA) for diagnosing cervical lymph node metastasis. Methods: A total of 110 PTC patients with pathologically confirmed cervical lymph node metastasis in Ruijin hospital during June 2020 to December 2020, were enrolled (study group). Another 110 PTC patients without cervical lymph node metastasis were served as the control group. The expression level of ENST00000489676 in FNA biopsy samples from 220 patients were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The correlation of ENST00000489676 expression with lymph node metastasis in PTC patients was explored. ROC curve was used to calculate the cutoff value of ENST00000489676 expression level for diagnosing cervical lymph node metastasis of PTC in puncture specimens. The sensitivity, specificity and accuracy of ultrasonography alone and combined with ENST00000489676 detection in FNA biopsy samples for diagnosis of lymph node metastasis were also calculated respectively. Results: The expression of ENST0000048967 in study group was significantly lower than that in non-lymph node metastasis group(t=-4.198, P<0.001). The statistical results showed that the low expression of ENST000000489676 was positively correlated with PTC lymph node metastasis. The optimal cutoff value of ENST00000489676 for diagnosing cervical lymph node metastasis of PTC in the FNAB specimen was 2.0950 by ROC curve. The sensitivity and accuracy of ultrasonography combined with ENST00000489676 detection for diagnosising lymph node metastasis were higher than those of ultrasonography alone, respectively(sensitivity, 61.11% and 17.27%; specificity, 63.39% and 86.36%; accuracy, 62.27%, and 51.82%). Conclusions: Low expression of ENST00000489676 is significantly correlated with lymph node metastasis of PTC. ENST00000489676 may serve as a molecular marker to assist ultrasonography diagnosis of cervical lymph node metastasis for PTC.

Key words： Papillary thyroid carcinoma; Lymph node metastasis; ENST00000489676; Ultrasonography; US-fine needle aspiration biopsy

Cite this article

WANG Wenhan, XIA Shujun, ZHAN Weiwei . Application of long non-coding RNA ENST00000489676 detection in ultrasonographic evaluation of cervical lymph node metastasis in papillary thyroid carcinoma[J]. Journal of Diagnostics Concepts & Practice, 2022 , 21(04) : 514 -519 . DOI: 10.16150/j.1671-2870.2022.04.016

References

[1] Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J]. CA Cancer J Clin, 2021, 71(3):209-249.
[2] Haugen BR, Alexander EK, Bible KC, et al. 2015 Ame-rican Thyroid Association Management Guidelines for Adult Patients with Thyroid Nodules and Differentiated Thyroid Cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer[J]. Thyroid, 2016, 26(1):1-133.
[3] Limberg J, Ullmann TM, Stefanova D, et al. Does Aggressive Variant Histology Without Invasive Features Predict Overall Survival in Papillary Thyroid Cancer?: A National Cancer Database Analysis[J]. Ann Surg, 2021, 274(3):e276-e281.
[4] O′Grady TJ, Gates MA, Boscoe FP. Thyroid cancer incidence attributable to overdiagnosis in the United States 1981-2011[J]. Int J Cancer, 2015, 137(11):2664-2673.
[5] Colonna M, Uhry Z, Guizard AV, et al. Recent trends in incidence, geographical distribution, and survival of pa-pillary thyroid cancer in France[J]. Cancer Epidemiol, 2015, 39(4):511-518.
[6] Feng JW, Yang XH, Wu BQ, et al. Predictive factors for central lymph node and lateral cervical lymph node metastases in papillary thyroid carcinoma[J]. Clin Transl Oncol, 2019, 21(11):1482-1491.
[7] Qu N, Zhang L, Ji QH, et al. Risk Factors for Central Compartment Lymph Node Metastasis in Papillary Thyroid Microcarcinoma: A Meta-Analysis[J]. World J Surg, 2015, 39(10):2459-2470.
[8] Mulla M, Schulte KM. Central cervical lymph node metastases in papillary thyroid cancer: a systematic review of imaging-guided and prophylactic removal of the central compartment[J]. Clin Endocrinol (Oxf), 2012, 76(1):131-136.
[9] Lee EW, Chen C, Sauk S, et al. How diagnostic is ultrasound-guided neck mass biopsy (fine-needle capillary sampling biopsy technique)?: evaluation of 132 nonthyroid neck mass biopsies with pathologic analysis over 7 years at a single institution[J]. J Ultrasound Med, 2009, 28(12):1679-1684.
[10] Kopp F, Mendell JT. Functional Classification and Experimental Dissection of Long Noncoding RNAs[J]. Cell, 2018, 172(3):393-407.
[11] Boon RA, Jaé N, Holdt L, et al. Long Noncoding RNAs: From Clinical Genetics to Therapeutic Targets?[J]. J Am Coll Cardiol, 2016, 67(10):1214-1226.
[12] Winkler L, Dimitrova N. A mechanistic view of long noncoding RNAs in cancer[J]. Wiley Interdiscip Rev RNA, 2022, 13(3):e1699.
[13] Gutschner T, Diederichs S. The hallmarks of cancer: a long non-coding RNA point of view[J]. RNA Biol, 2012, 9(6):703-719.
[14] Yan H, Bu P. Non-coding RNA in cancer[J]. Essays Biochem, 2021, 65(4):625-639.
[15] 陈滢, 何莉莉, 蒋锋. 长链非编码RNA FENDRR在结肠直肠癌中的表达及意义[J]. 诊断学理论与实践, 2018, 17(1):87-91.
[15] He LL, Jiang F. Expression of long non-coding RNA FENDRR in human colorectal cancer and its correlation with prognosis[J]. J Diagn Concepts & Pract, 2018, 17(1):87-91.
[16] Zhou T, Zhong M, Zhang S, et al. LncRNA CASC2 expression is down- regulated in papillary thyroid cancer and promotes cell invasion by affecting EMT pathway[J]. Cancer Biomark, 2018, 23(2):185-191.
[17] Xia E, Bhandari A, Shen Y, et al. LncRNA CCND2-AS1 promotes proliferation, migration, and invasion in papillary thyroid carcinoma[J]. Biochem Biophys Res Commun, 2018, 496(2):628-632.
[18] Xiang J, Guan Y, Bhandari A, et al. LINC00511 influe-nces cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma[J]. J Cancer, 2020, 11(2):450-459.
[19] Wang W, Xia S, Zhan W. The Long Non-Coding RNA ENST00000489676 Influences Papillary Thyroid Cancer Cell Proliferation and Invasion through Regulating MiR-922[J]. J Cancer, 2019, 10(22):5434-5446.
[20] Gupta RA, Shah N, Wang KC, et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J]. Nature, 2010, 464(7291):1071-1076.
[21] Yang Z, Zhou L, Wu LM, et al. Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carcinoma patients following liver transplantation[J]. Ann Surg Oncol, 2011, 18(5):1243-1250.
[22] Liu FT, Qiu C, Luo HL, et al. The association of HOTAIR expression with clinicopathological features and prognosis in gastric cancer patients[J]. Panminerva Med, 2016, 58(2):167-174.
[23] Wang Y, Xue D, Li Y, et al. The Long Noncoding RNA MALAT-1 is A Novel Biomarker in Various Cancers: A Meta-analysis Based on the GEO Database and Literature[J]. J Cancer, 2016, 7(8):991-1001.
[24] Zhang R, Xia Y, Wang Z, et al. Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer[J]. Biochem Biophys Res Commun, 2017, 490(2):406-414.
[25] Goyal B, Yadav SRM, Awasthee N, et al. Diagnostic, prognostic, and therapeutic significance of long non-co-ding RNA MALAT1 in cancer[J]. Biochim Biophys Acta Rev Cancer, 2021, 1875(2):188502.
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