Application of long non-coding RNA ENST00000489676 detection in ultrasonographic evaluation of cervical lymph node metastasis in papillary thyroid carcinoma

doi:10.16150/j.1671-2870.2022.04.016

Abstract

Abstract:

Objective: To analyze the value of lncRNA ENST0000048967 detection in biopsy tissue of papillary thyroid carcinoma (PTC) by ultrasound-guided fine-needle aspiration (US-FNA) for diagnosing cervical lymph node metastasis. Methods: A total of 110 PTC patients with pathologically confirmed cervical lymph node metastasis in Ruijin hospital during June 2020 to December 2020, were enrolled (study group). Another 110 PTC patients without cervical lymph node metastasis were served as the control group. The expression level of ENST00000489676 in FNA biopsy samples from 220 patients were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The correlation of ENST00000489676 expression with lymph node metastasis in PTC patients was explored. ROC curve was used to calculate the cutoff value of ENST00000489676 expression level for diagnosing cervical lymph node metastasis of PTC in puncture specimens. The sensitivity, specificity and accuracy of ultrasonography alone and combined with ENST00000489676 detection in FNA biopsy samples for diagnosis of lymph node metastasis were also calculated respectively. Results: The expression of ENST0000048967 in study group was significantly lower than that in non-lymph node metastasis group(t=-4.198, P<0.001). The statistical results showed that the low expression of ENST000000489676 was positively correlated with PTC lymph node metastasis. The optimal cutoff value of ENST00000489676 for diagnosing cervical lymph node metastasis of PTC in the FNAB specimen was 2.0950 by ROC curve. The sensitivity and accuracy of ultrasonography combined with ENST00000489676 detection for diagnosising lymph node metastasis were higher than those of ultrasonography alone, respectively(sensitivity, 61.11% and 17.27%; specificity, 63.39% and 86.36%; accuracy, 62.27%, and 51.82%). Conclusions: Low expression of ENST00000489676 is significantly correlated with lymph node metastasis of PTC. ENST00000489676 may serve as a molecular marker to assist ultrasonography diagnosis of cervical lymph node metastasis for PTC.

Key words: Papillary thyroid carcinoma, Lymph node metastasis, ENST00000489676, Ultrasonography, US-fine needle aspiration biopsy

CLC Number:

R541.7

WANG Wenhan, XIA Shujun, ZHAN Weiwei. Application of long non-coding RNA ENST00000489676 detection in ultrasonographic evaluation of cervical lymph node metastasis in papillary thyroid carcinoma[J]. Journal of Diagnostics Concepts & Practice, 2022, 21(04): 514-519.

Figures/Tables 4

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Gene/shRNA		序列（5′→3′）
ENST00000489676	正向引物	GGCAGCTGACAGGGCTTT
	逆向引物	GCAGTCCACCCTGTCCTTG
GAPDH	正向引物	AAGGTGAAGGTCGGAGTCAAC
	逆向引物	GGGGTCATTGATGGCAACAATA

特征	高表达组（113例）	低表达组（107例）	P值	χ²值
性别			0.214	1.543
男	29(13.18%)	20(9.09%)
女	84(38.18%)	87(39.55%)
年龄（岁）			0.962	0.002
<45	69(31.36%)	65(29.55%)
≥45	44(20.00%)	42(19.09%)
最大径（mm）	11.31±5.88	9.88±6.65
<10	54(24.55%)	64(29.09%)	0.074	3.196
≥10	59(26.82%)	43(19.54%)
多灶癌			0.555	0.349
否	73(33.18%)	65(29.55%)
是	40(18.18%)	42(19.09%)
形态			0.883	0.022
不规则	91(41.36%)	87(39.55%)
规则	22(10.00%)	20(9.09%)
A/T			0.198	1.658
<1	76(34.55%)	63(28.63%)
≥1	37(16.82%)	44(20.00%)
边缘			0.685	0.165
不光整	104(47.27%)	100(45.45%)
光整	9(4.09%)	7(3.19%)
内部回声			1.000	0.000
非低回声	2(0.91%)	1(0.46%)
低回声	111(50.45%)	106(48.18%)
内部结构			0.962	0.002
非实性	1(0.46%)	2(0.91%)
实性	112(50.91%)	105(47.72%)
钙化			0.376	0.784
非微钙化	43(19.55%)	47(21.36%)
微钙化	70(31.82%)	60(27.27%)
血供			0.241	4.195
无	8(3.64%)	13(5.91%)
低	78(35.45%)	60(27.27%)
中	21(9.55%)	26(11.82%)
高	6(2.72%)	8(3.64%)
转移			0.001	13.215
有	42(19.09%)	66(30.00%)
无	71(32.27%)	41(18.64%)