内科理论与实践

内科理论与实践 ›› 2025, Vol. 20 ›› Issue (01): 18-23.doi: 10.16138/j.1673-6087.2025.01.04

• 论著 • 上一篇    下一篇

基于网络药理学及转录组学揭示虎黄洗剂促进糖尿病创面愈合机制

张杰1, 徐顺2, 刘琰1, 施燕1()   

  1. 1.上海交通大学医学院附属瑞金医院灼伤整形科，上海 200025
    2.上海中医药大学附属第七人民医院烧伤整形科，上海 200137
  • 收稿日期:2024-11-05 出版日期:2025-02-28 发布日期:2025-04-30
  • 通讯作者: 施燕 E-mail:18516130422@163.com
  • 基金资助:
    上海市“科技创新行动计划”启明星项目(22YF1424900);国家自然科学基金项目(82302802);国家自然科学基金项目(82202457);上海市生物医药领域定向项目(22DX1900600);上海市重中之重研究中心项目(2023ZZ02013);浦东新区卫生健康委员会临床特色学科建设计划(PWYts2021-16)

Exploring the mechanism of Huhuang decoction promoting diabetic wound healing based on network pharmaco-logy and transcriptomics

ZHANG Jie1, XU Shun2, LIU Yan1, SHI Yan1()   

  1. 1. Department of Burn, Shanghai Burn Institute, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    2. Department of Burn and Plastic Surgery, Seventh People's Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China
  • Received:2024-11-05 Online:2025-02-28 Published:2025-04-30
  • Contact: SHI Yan E-mail:18516130422@163.com

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摘要：

目的：探讨虎黄洗剂对糖尿病创面愈合的影响及作用机制。方法：通过中药系统药理学数据库分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, TCMSP)数据库查询虎黄洗剂各组分的有效成分及靶基因。通过比较毒理基因组学数据库(Comparative Toxicogenomics Database, CTD)及GeneCards数据库筛选糖尿病足疾病靶点。采用Cytoscape软件构建成分-靶点相互作用网络。使用DAVID在线工具进行基因本体(gene ontology, GO)及京都基因和基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析。对糖尿病大鼠创面组织采用转录组学测序分析。动物实验验证虎黄洗剂对糖尿病大鼠创面愈合的影响，酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测创面组织炎症因子白细胞介素(interleukin,IL)-6、肿瘤坏死因子(tumor necrosis factor, TNF)-α及IL-1β表达。结果：网络药理学及转录组学测序分析表明，虎黄洗剂潜在靶点与炎症调控密切相关。创面局部应用虎黄洗剂可促进糖尿病创面愈合，苏木精-伊红(hematoxylin and eosin, HE)染色结果显示虎黄洗剂可促进糖尿病创面上皮化，ELISA检测结果提示虎黄洗剂可抑制创面组织炎症因子IL-6、TNF-α及IL-1β表达。结论：虎黄洗剂可抑制糖尿病创面炎症反应，促进创面愈合。

关键词: 虎黄洗剂, 糖尿病创面, 网络药理学, 转录组学

Abstract:

Objective To investigate the effect and mechanism of Huhuang decoction on diabetic wound healing.Methods The active ingredients and target genes of each component of Huhuang decoction were queried through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database. The gene targets of diabetic foot ulcer were screened through the Comparative Toxicogenomics Database (CTD) and GeneCards database. The component-target interaction network was drawn using Cytoscape software draw and gene ontology（GO） and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed using DAVID. The wound tissue of diabetic rats was collected to undergo transcriptomic sequencing analysis. The effect of Huhuang decoction on wound healing of diabetic rats was verified in vivo, and the expressions of inflammatory factors interleukin 6 (IL-6), tumor necrosis factor α (TNF-α) and IL-1β in wound tissue were detected by enzyme-linked immunosorbent assay (ELISA). Results Network pharmacology and transcriptomic sequencing analysis showed that the potential targets of Huhuang decoction were closely related to inflammatory regulation. Local application of Huhuang decoction on the wound surface could promote the healing of diabetic wounds. Hematoxylin and eosin （HE） staining results displayed that Huhuang decoction promoted epithelialization of diabetic wounds.ELISA test results showed that Huhuang decoction could inhibit the expression of inflammatory factors IL-6, TNF-α and IL-1β in wound tissue. Conclusions Huhuang decoction could inhibit inflammation of diabetic wounds and promote wound healing.

Key words: Huhuang decoction, Diabetic wounds, Network pharmacology, Transcriptomics

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