外泌体miRNA146b-5p促进甲状腺乳头状癌细胞迁移和侵袭

doi:10.16139/j.1007-9610.2019.04.014

外科理论与实践 ›› 2019, Vol. 24 ›› Issue (04): 344-348.doi: 10.16139/j.1007-9610.2019.04.014

外泌体miRNA146b-5p促进甲状腺乳头状癌细胞迁移和侵袭

叶卫东, 邓先兆^*, 樊友本

上海交通大学附属第六人民医院普外科甲状腺甲状旁腺中心,上海 200233

收稿日期:2018-11-26 出版日期:2019-07-25 发布日期:2019-08-25
通讯作者: 樊友本,E-mail: fanyouben2006@163.com
作者简介:^*：共同第一作者
基金资助:
上海交通大学附属第六人民医院预研基金（LYNYLC-0049）

Exosomal miRNA146b-5p promotes migration and invasion of papillary thyroid carcinoma cell

YE Weidong, DENG Xianzhao, FAN Youben

Department of General Surgery, Center of Thyroid and Parathyroid, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China

Received:2018-11-26 Online:2019-07-25 Published:2019-08-25

摘要/Abstract

摘要： 目的： 分离和鉴定甲状腺乳头状癌KTC-1细胞分泌的外泌体,并探讨外泌体携带miRNA146b-5p对KTC-1细胞体外迁移和侵袭的影响。方法： 收集KTC-1细胞的外泌体,电转miRNA146b-5p和其抑制剂进入外泌体,并与KTC-1细胞共培养。检测KTC-1细胞的体外迁移和侵袭的能力。结果： 成功分离和鉴定KTC-1细胞分泌的外泌体。外泌体miRNA146b-5p可进入KTC-1细胞,促进KTC-1细胞的体外迁移和侵袭。结论： 外泌体miRNA146b-5p可促进甲状腺乳头状癌的体外侵袭和转移。外泌体miRNA423-5p沉默可能作为甲状腺乳头状癌的治疗方法。

关键词: 甲状腺乳头状癌, 外泌体, miRNA146b-5p, 迁移和侵袭

Abstract: Objective To isolate and identify the exosomes secreted by papillary thyroid carcinoma (PTC) KTC-1 cells, and to investigate the effects of miRNA146b-5p in exosomes on the migration and invasion in vitro. Methods Exosomes secreted by KTC-1 cells were collected and identified. miRNA 146b-5p and its inhibitor were electroporated into exosomes and co-cultured with KTC-1 cells. Migration and invasion in vitro were examined. Results Exosomes secreted by KTC-1 cells were successfully isolated and identified. Exosomal miRNA146b-5p could enter KTC-1 cells and promote migration and invasion of KTC-1 cells in vitro. Conclusions Exosomal miRNA146b-5p can promote the migration and invasion of PTC in vitro. miRNA146b-5p silencing in exosomes might represent a potential approach against PTC.

Key words: Papillary thyroid carcinoma, Exosome, miRNA146b-5p, Migration and invasion

中图分类号:

R736.1

叶卫东, 邓先兆, 樊友本. 外泌体miRNA146b-5p促进甲状腺乳头状癌细胞迁移和侵袭[J]. 外科理论与实践, 2019, 24(04): 344-348.

YE Weidong, DENG Xianzhao, FAN Youben. Exosomal miRNA146b-5p promotes migration and invasion of papillary thyroid carcinoma cell[J]. Journal of Surgery Concepts & Practice, 2019, 24(04): 344-348.

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外泌体miRNA146b-5p促进甲状腺乳头状癌细胞迁移和侵袭

Exosomal miRNA146b-5p promotes migration and invasion of papillary thyroid carcinoma cell

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