Study on serum exosome proteins in metastatic breast cancer patients

doi:10.16139/j.1007-9610.2019.02.012

Abstract

Abstract: Objective To study differentially expressed protein in serum exosome of the patients with both metastatic and non-metastatic breast cancer. Methods Serum was collected from both 25 breast cancer patients with metastasis of lymph node or distant organ (M+ group) and 25 breast cancer patients without any metastasis (M- group). Serum exosomes were separated with EIQ3 analytic kit in 4 breast cancer patients in M+ group and 4 breast cancer patients in M- group.These serum exosomes were recognized by CD9 and CD63 as surface markers using western blotting, and the size of exosomes were meaured by nanosight tracking analysis（NTA）. Protein mass spectrometry and bioinformatics analysis were performed to identify the differentially expressed proteins in serum exosomes of breast cancer patients, which were also quantified by Western blot. Results The particle size by nanosight tracking analysis ranged from 20 to 200 nm and the expression of CD9 and CD63 were positive. Totally there were 301 proteins identified by mass spectrometry and among them 16 proteins differentially expressed from serum exosomes in M+ group and M- group. The expression of vitronectin(VN) and kallistatin (KAL) was up-regulated, while the expression of obscurin(OBSCN) was down-regulated in M+ group compared with those in M- group using Western blotting, which was consistent with the results of analysis by mass spectrometry. Conclusions The serum exosomes in breast cancer patients are present identified by particle size and markers. VN, KAL and OBSCN in exosomes could serve as potential candidate biomarkers for prediction of metastatic breast cancer.

Key words: Breast cancer, Exosome, Mass spectrum, Biomarker

CLC Number:

R737.9

CAO Lichen, TIAN Baoxing, QU Feilin, ZHANG Qinghua, GUO Shanyu. Study on serum exosome proteins in metastatic breast cancer patients[J]. Journal of Surgery Concepts & Practice, 2019, 24(02): 141-148.

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