组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2014, Vol. 10 ›› Issue (5): 247-265.doi: 10.3969/j.issn.1673-0364.2014.05.003

• 论著 • 上一篇    下一篇

表达hTERT基因大鼠骨髓间充质干细胞的类肝样细胞分化能力研究

马迪，施敏敏，严佶祺，陈雪华，计骏，张佳年，彭承宏   

  1. 上海交通大学医学院附属瑞金医院普外科
  • 发布日期:2020-07-23

Hepatic Differentiation Potential of hTERT Gene Transfected Bone Marrow Mesenchymal Stem Cells in Rats

MA Di,SHI Minmin,YAN Jiqi,CHEN Xuehua,JI Jun,ZHANG Jianian,PENG Chenghong   

  1. Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine;
  • Published:2020-07-23
  • Contact: 国家自然科学基金（81070358）

摘要： 目的对表达hTERT基因的大鼠骨髓间充质干细胞(Bone mesenchymal stem cells,BMSCs)进行成瘤风险评估及类肝样细胞分化能力的鉴定。方法将表达hTERT基因的大鼠BMSCs进行裸鼠致瘤试验评估成瘤风险,通过序贯法诱导成肝分化,于诱导后观察细胞形态变化、检测ALB和AFP的表达、糖原染色鉴定糖原储备能力。结果裸鼠致瘤实验提示表达hTERT基因的大鼠BMSCs无成瘤风险。成肝诱导后,细胞形态逐渐变为圆形;ALB仅在诱导第21天部分阳性表达,AFP在诱导第14天起阳性表达;糖原染色阳性。结论表达hTERT基因的大鼠BMSCs无致瘤风险,且具有类肝样细胞的分化能力。

关键词: 人端粒酶逆转录酶, 骨髓间充质干细胞, 类肝样细胞

Abstract: Objective To estimate the oncogenicity of hTERT gene transfected rat BMSCs and to identify its hepatic differentiation potential. Methods The oncogenicity of hTERT gene transfected rat BMSCs was estimated by tumorigenesis test in nude mouse. Then the hTERT gene transfected rat BMSCs were induced for hepatic differentiation by sequential method. The change of cell morphology was observed, the expression of ALB and AFP were detected, and the ability of glycogen storage was tested by PAS stain. Results Tumorigenesis test of nude mouse suggested no oncogenic risk of hTERT gene transfected rat BMSCs. After hepatic induction, the induced cells became round in shape. A few ALB （＋） cells were observed only at D21 after induction while more and more AFP （＋） cells were observed started from D14 after induction. The result of PAS stain was positive. Conclusion The hTERT gene transfected rat BMSCs have the potential of hepatic differentiation with no oncogenicity.

Key words: Human telomerase reverse transcriptase, Bone marrow mesenchymal stem cells, Hepatocyte-like cells

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