组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2013, Vol. 9 ›› Issue (5): 256-259.doi: 10.3969/j.issn.1673-0364.2013.05.004

• 论著 • 上一篇    下一篇

表达HTERT的大鼠骨髓间充质干细胞生物学特性研究

马迪，严佶祺，彭承宏，施敏敏，陈雪华，王维杰，李宏为   

  1. 上海交通大学医学院附属瑞金医院普外科
  • 发布日期:2020-07-23

Biological Characteristics of Bone Marrow Mesenchymal Stem Cells Expressing hTERT Gene

MA Di,YAN Jiqi,PENG Chenghong,SHI Minmin,CHEN Xuehua,WANG Weijie,LI Hongwei   

  1. Department of General Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine
  • Published:2020-07-23
  • Contact: 国家自然基金（81070358）；上海市自然基金（09zr1418600）

摘要： 目的将含hTERT基因的重组慢病毒液感染大鼠骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs),鉴定其生物学特性。方法分别将含hTERT基因重组慢病毒液、空病毒液感染大鼠BMSCs,实验分为MSChTERT组、MSC-GFP组和MSC组(未感染组)。体外培养扩增后,通过定量PCR对各组目的基因表达进行检测。观察MSC组及MSC-hTERT组的体外传代次数及细胞形态变化,分析细胞周期。流式细胞仪检测MSC-hTERT组细胞标志物,评估其成骨、成脂分化能力。结果MSC-hTERT组目的基因mRNA表达均高于另两组,体外培养过程中MSChTERT组传代次数和增殖指数高于MSC组,且具备多向分化潜能。结论转染hTERT的BMSCs具备BMSCs同样的生物学特性,且细胞增殖能力加强,可为组织工程研究以及细胞移植治疗提供可靠的种子细胞。

关键词: 人端粒酶逆转录酶, 骨髓间充质干细胞, 生物学特性, 分化潜能

Abstract: Objective To identify the biological characteristics after infecting the rat BMSCs with recombinant virus supernatant containing hTERT gene. Methods BMSCs were infected with hTERT recombinant virus supernatant (MSC-hTERT group) and empty virus supernatant (MSC-GFP group), the uninfected group (MSC group) was also set up. The expression of target gene was detected by realtime PCR. Number of passages, cell morphology and cell cycle in MSC group and MSC-hTERT group were observed and analyzed. Cell markers of cells in MSC-hTERT group were detected and the potential of differentiation was also evaluated. Results The expression of hTERT was higher in MSC-hTERT group than in the other two groups. Number of passages and proliferation index in MSC-hTERT group were higher than in MSC group. The ability of multi-lineage differentiation potential of cells in MSC-hTERT group was maintained. Conclusion MSCs transfected by hTERT have the same biological characteristics as primary BMSCs and the ability of cell proliferation was strengthened. More cells could be reserved for tissue engineering and cell transplantation therapy.

Key words: Human telomerase reverse transcriptase, Bone marrow mesenchymal stem cells, Biological characteristics, Potential of differentiation

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