关键词: 口腔黏膜病, 微小核糖核酸, 成纤维细胞, 机制研究

Abstract: Objective To explore the effect of miR-2053 on fibroblasts in oral mucosal repair and its mechanism.Methods Forty patients with oral mucosal disease were selected as the case group, and 40 healthy subjects from the physical examination center were selected as the control group. Real-time quantitative PCR was used to verify the expression of miR-2053 in oral mucosa. The human gingival fibroblasts were transfected with miR-2053. The cell viability was detected by CCK-8 method. The cell mobilization rate was detected by flow cytometry and the cell migration rate was detected by Transwell chamber method. RT-PCR and Western-blot were used to detect the key transcription factors Collagen Ⅰ,Collagen Ⅲ, MMP-1, MMP-13 and their protein expression levels in tissue remodeling. Results Compared with the control group, the expression of miR-2053 in oral mucosa was significantly increased(P<0.05). Cell viability and migration rate of human gingival fibroblast model transfected with miR-2053 were both significantly lower than that of the control group and the negative analogue group(P <0.05), and the apoptosis was significantly increased(P <0.05). The expression levels of Collagen Ⅰ, Collagen Ⅲ, MMP-1 and MMP-13 in human gingival fibroblasts transfected with miR-2053 were significantly decreased compared with the control group and the negative analogue group(P <0.05). Further detection of protein levels revealed that the relative expression levels of Collagen Ⅰ, Collagen Ⅲ and MMP-13 in human gingival fibroblasts transfected with miR-2053 were significantly lower than those in the control group and the negative analogue group(P<0.05).Conclusion miR-2053 may affect the vitality, apoptosis and migration of human gingival fibroblasts by affecting the expression of Collagen Ⅰ, Collagen Ⅲ, MMP-1 and MMP-13, thus affect the rehabilitation of oral mucosal disease and the disease course.

Key words: Oral mucosal disease, MicroRNA, Fibroblasts, Mechanism study