组织工程与重建外科杂志

组织工程与重建外科杂志 ›› 2015, Vol. 11 ›› Issue (2): 90-94.doi: 10.3969/j.issn.1673-0364.2015.02.011

• 论著 • 上一篇    下一篇

骨髓单个核细胞与骨髓间充质干细胞促进大鼠皮瓣存活率的比较

许鹏，唐郑雅，陆阳，周广东，刘伟，曹谊林，张文杰   

  1. 上海交通大学医学院附属第九人民医院整复外科,上海市组织工程研究重点实验室；组织工程国家工程中心
  • 发布日期:2020-07-23

Comparison between Bone Marrow-derived Mononuclear Cells and Mesenchymal Stem Cells in Promoting Survival Rates of Random-pattern Skin Flap in Rats

XU Peng,TANG Zhengya,LU Yang,ZHOU Guangdong,LIU Wei,CAO Yilin,ZHANG Wenjie   

  1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering;National Tissue Engineering Center of China;
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划（“973”项目）（2011cb964704）；国家自然科学基金（81271714,31170944）

摘要： 目的比较来源于等量骨髓的单个核细胞与经扩增的间充质干细胞,促进大鼠随意皮瓣成活率的效果。方法取等质等量的大鼠骨髓,一半直接离心获得骨髓单个核细胞,一半经体外培养获得骨髓间充质干细胞,注射相等数量(n=6)的大鼠随意皮瓣。术后测量并计算皮瓣成活面积,取材,行组织学检测,计数CD31阳性血管数量。结果未经培养的骨髓单个核细胞组皮瓣的平均存活率为(71.6±8.4)%,培养的骨髓间充质干细胞组平均存活率为(66.2±3.1)%,这两组存活率均显著高于注射平衡液的对照组(55.9±3.4)%;注射细胞组之间平均存活率没有统计学差异。组织学血管密度计数显示,骨髓单个核细胞组和骨髓间充质干细胞组的微血管数量分别是(58.2±6.8)和(42.7±5.1),都显著高于PBS对照组(22.8±3.1),而骨髓单个核细胞组也显著高于骨髓间充质干细胞组。结论与不经培养的骨髓单个核细胞相比,通过体外扩增的骨髓间充质细胞,未能显著提高大鼠随意皮瓣的成活率。

关键词: 随意皮瓣, 细胞治疗, 骨髓单个核细胞, 骨髓间充质干细胞

Abstract: Objective To compare the non-cultured bone marrow mononuclear cells (BM-MNCs) and cultured bone marrow mesenchymal stem cells (BMSCs) in the promotion of random-pattern skin flap survival of rats. Methods BM-MNCs and BMSCs derived from two identical rat bone marrow aspirates were injected in a random-pattern skin flap model of rat (n=6). The survival area was determined by its appearance, color and texture. Capillary density of flaps was measured by histology. Results The flap survival rates were (71.6±8.4)%in the BM-MNC-treated group and (66.2±3.1)%in the BMSC-treated group, both of which were significantly higher than the control group (55.9 ±3.4)%. However, no significant difference was observed between the cell transplanted groups. According to vessel density assay, capillary density in the BM-MNC-treated group (58.2±6.8) was higher than in the BMSC-treated group (42.7±5.1), both of which were significantly higher than the control group (22.8±3.1). Conclusion Pre-culture of BMSCs does not bring therapeutic benefits.

Key words: Random-pattern skin flap, Cell therapy, Bone marrow mononuclear cells, Bone marrow mesenchymal stem cells

中图分类号: