Experimental Study of Human Umbilical Cord Mesenchymal Stem Cells on Differentiation of Vascular Endothelial Progenitor Cells in Vitro

doi:10.3969/j.issn.1673-0364.2009.006.003

Journal of Tissue Engineering and Reconstructive Surgery ›› 2009, Vol. 5 ›› Issue (6): 310-313.doi: 10.3969/j.issn.1673-0364.2009.006.003

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Experimental Study of Human Umbilical Cord Mesenchymal Stem Cells on Differentiation of Vascular Endothelial Progenitor Cells in Vitro

CHEN En¹, ZHU Jiaxue², QIN Jinbao³, DING Wenxiang¹, YU Xiaoqing¹, GAO Botao¹

1 Department of Cardio-Thoracic Surgery; Shanghai Children’s Medical Center Affiliated Shanghai Jiaotong University School of Medicine; Shanghai 200127; China; 2 Department of Plastic Surgery; First People’s Hospital of Shanghai Jiaotong University School of Medicine; Shanghai 200080; China; 3 Department of Orthopaedics; Shanghai Children’s Medical Center Affiliated Shanghai Jiaotong University School of Medicine; Shanghai 200127; China.

Published:2020-07-23
Contact: 国家自然科学基金(30672088)

Abstract

Abstract: Objective To explore the differentiation of human umbilical cord mesenchymal stem cells(UMSCs).Methods The UCMSCs were isolated from full-term neonates.MSCs were obtained by mixed enzymatic digestion and cultured in DMEM/10%FBS.Surface antigens of UCMSCs were detected by flow cytometry.Different passages of MSCs(P3-P6) were induced to differentiate towards endothelial progenitor cells by VEGF and b-FGF,then endothelial cells markers were detected by immunofluorescence.Results Human umbilical cord mesenchymal stem cells can be induced to endothelial cells lineage.Immunofluorescent assay showed that different passages of MSCs could express CD34,CD133 and vWF and Dil-acLDL assay was positive.Conclusion Human umbilical cord mesenchymal stem cells could be induced into vascular endothelial cells,which could be used as a new source of seeding cells for tissue engineered blood vessel and cardiac valve.

Key words: Human umbilical cord mesenchymal stem cells, Vascular endothelial progenitor cells, Differentiation, Tissue engineering

CLC Number:

R329

CHEN En, ZHU Jiaxue, QIN Jinbao, DING Wenxiang, YU Xiaoqing, GAO Botao. Experimental Study of Human Umbilical Cord Mesenchymal Stem Cells on Differentiation of Vascular Endothelial Progenitor Cells in Vitro[J]. Journal of Tissue Engineering and Reconstructive Surgery, 2009, 5(6): 310-313.

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Experimental Study of Human Umbilical Cord Mesenchymal Stem Cells on Differentiation of Vascular Endothelial Progenitor Cells in Vitro

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