Journal of Tissue Engineering and Reconstructive Surgery ›› 2017, Vol. 13 ›› Issue (1): 1-4.doi: 10.3969/j.issn.1673-0364.2017.01.001

• Original article •     Next Articles

The Construction of Tissue Engineered Cartilage by Using GT/PCL Nanofibres Membranes in Porcine Model

HUO Yingying1,2,3, ZHOU Guangdong2,3, ZEHNG Rui1,2,3   

  1. 1 Department of Dermatology, Shanghai Ninth People "s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China; 2 Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China; 3 National Tissue Engineering Center of China, Shanghai 200241, China)
  • Published:2020-07-23
  • Contact: 国家自然科学基金

Abstract: Objective To investigate the possibility of the tissue engineered cartilage construction with electrospun gelatin/polycaprolactone (GT/PCL) membranes in subcutaneous of porcine model. Methods Engineered cartilage was constructed by auricular chondrocytes of porcine as seed cells with GT/PCL membranes (experimental group) and PGA/PLA material (control group) as scaffold. Then the engineered cartilage was implanted subcutaneously in the abdominal wall of swine after cultured 3 weeks in vitro. The samples of the two groups were collected at two time points:3 weeks in vitro and 3 weeks in vivo. Histological observation, wet weight and mechanical strength test were used to evaluate the chondrogenesis. Results After 3 weeks cultured in vitro, cartilage phenotype and undegraded scaffolds were observed in two groups by histological analysis. And the extracellular matrix in control group seems more uniform than the experimental group. However, After 3 weeks cultured in vivo, mature cartilage were only formed in the experimental group, and fibrous tissue substitute for cartilage was observed in the control group. In addition, the engineered cartilage in experimental group showed better mechanical strength. Conclusion GT/PCL membranes could be successfully used in the construction of tissue engineering cartilage in subcutaneous of large animal. It could be a kind of promising scaffold for cartilage tissue engineering.

Key words: Tissue engineered cartilage, Electrospun nanofibres membranes, Polyglycolic acid, Polylactic acid, Inflammatory response

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