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Table of Content

    23 July 2020, Volume 13 Issue 1 Previous Issue    Next Issue
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    Original article
    The Construction of Tissue Engineered Cartilage by Using GT/PCL Nanofibres Membranes in Porcine Model
    HUO Yingying, ZHOU Guangdong, ZEHNG Rui
    2017, 13 (1):  1-4.  DOI: 10.3969/j.issn.1673-0364.2017.01.001
    Abstract ( 469 )  
    Objective To investigate the possibility of the tissue engineered cartilage construction with electrospun gelatin/polycaprolactone (GT/PCL) membranes in subcutaneous of porcine model. Methods Engineered cartilage was constructed by auricular chondrocytes of porcine as seed cells with GT/PCL membranes (experimental group) and PGA/PLA material (control group) as scaffold. Then the engineered cartilage was implanted subcutaneously in the abdominal wall of swine after cultured 3 weeks in vitro. The samples of the two groups were collected at two time points:3 weeks in vitro and 3 weeks in vivo. Histological observation, wet weight and mechanical strength test were used to evaluate the chondrogenesis. Results After 3 weeks cultured in vitro, cartilage phenotype and undegraded scaffolds were observed in two groups by histological analysis. And the extracellular matrix in control group seems more uniform than the experimental group. However, After 3 weeks cultured in vivo, mature cartilage were only formed in the experimental group, and fibrous tissue substitute for cartilage was observed in the control group. In addition, the engineered cartilage in experimental group showed better mechanical strength. Conclusion GT/PCL membranes could be successfully used in the construction of tissue engineering cartilage in subcutaneous of large animal. It could be a kind of promising scaffold for cartilage tissue engineering.
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    Inhibitory Effect of Capsaicin on the Proliferation of Keloid Fibroblasts Cultured in Vitro
    LIN Miaomiao,WANG Wenbo,KANG Wen,GAO Zhen,WU Xiaoli,TANG Shengjian,LIU Wei
    2017, 13 (1):  5-12.  DOI: 10.3969/j.issn.1673-0364.2017.01.002
    Abstract ( 588 )  
    Objective To explore the effect of capsaicin on inhibiting collagen expression, proliferation and migration ability of keloid fibroblasts in vitro and thus to provide scientific basis for clinical application of capsaicin in treatment of keloid. Methods The keloid tissues were harvested from 9 patients and digested with 0.3% collagenase to extract keloid fibroblasts. These cells were treated with different concentrations of capsaicin (0.5 μg/L and 5 μg/L) as the experiment group or without capsaicin treatment as the control group. CCK-8 assay was used to evaluate cell proliferation. Flow cytometry was also employed to evaluate cell cycle. Q-PCR assay was used to examine the gene expression levels of collagen and other extracellular matrices. In vitro wound scratch was also used to investigate cell migration ability. Results Capsaicin inhibited the proliferation of keloid fibroblasts, resulting in relative blockade of cell cycle in G0/G1 phase. It also inhibited the gene expression of collagens Ⅰ and Ⅲ and fibronectin. In addition, Capsaicin also inhibited the gene expression of IL-6, IL-8 and TNF-α, but had no effect on cell migration. Conclusion Capsaicin could inhibit the proliferation and gene expression of collagen and inflammatory cytokines of cultured fibroblasts in vitro.
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    The Inhibitory Effects of Oxymatrine Coating Agent on Human Hypertrophic Scar
    WANG Aili,XU Shun,GU Yaohui,HUANG Jing,JIA Qing,CHEN Bo
    2017, 13 (1):  9-12.  DOI: 10.3969/j.issn.1673-0364.2017.01.003
    Abstract ( 441 )  
    Objective To investigate the inhibitory effects of Oxymatrine coating agent on hypertrophic scar. Methods Human hypertrophic scar tissues were subcutaneously transplanted into the back of 30 nude mice to create the animal model of hypertrophic scar. Ten days after the operation, the mice were distributed into the control group, Oxymatrine coating agent group and positive medicine group, and were treated with blank coating agent, Oxymatrine coating agent and triamcinolone acetonide acetate coating agent respectively. Drugs were applied 2 times a day for 8 weeks. The growth of the scar was observed and photographed every week, and the size was measured 10 days after operation, and 35, 49, 56 days after drug treatment. HE staining was used for histological observation. RT-PCR and western blot assessment were used to detect the expression of Collagen Ⅰ, Ⅲ and α-SM-Actin. Results After drug treatment for 35, 49 and 56 days, the scar sizes in Oxymatrine coating agent group shrunk obviously and were much smaller compared with control group and positive medicine group (P<0.05). According to RT-PCR assessment, the expression of Collagen Ⅰ,Ⅲandα-SM-Actin were declined sharply in Oxymatrine coating agent group and positive medicine group, compared with the control group. Conclusion Oxymatrine coating agent has obvious inhibitory effects on hypertrophic scar, and also has anti-fibrosis effect.
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    The Effects of ADAM10 in Craniofacial Intramembranous Ossification of Mice
    TAN Yu,LUO Wei,CAO Haifeng,FANG Bing,YANG Zhi
    2017, 13 (1):  13-39.  DOI: 10.3969/j.issn.1673-0364.2017.01.004
    Abstract ( 323 )  
    Objective To explore the effects of ADAM10 in craniofacial intramembranous ossification of mice. Methods The expression pattern of ADAM10 in mice mandibular was detected by immunofluorescence assay and western blot. Cre-loxp technique was used to detect the function of ADAM10 in intramembranous ossification areas during craniomaxillofacial development. ADAM10 conditional knock-out mice was evaluated by X-ray and von Kossa staining. The cell proliferation, apoptosis and osteoblast differentiation were detected by immunofluorescence assay. Results Immunofluorescence assay revealed that ADAM10 was primarily distributed in the maxillary and mandibular skeletal genesis zones. ADAM10 was abundantly expressed from E14.5 to postnatal day 7 (P7) and was significantly decreased during adulthood. On E15.5, the ADAM10 cKO mice exhibited a shorter snout, flatter posterior skull and smaller maxilla and mandible, compared with the control littermates. The X-ray examination and von Kossa staining shown that the ADAM10 cKO embryos exhibited significantly reduced mineral bone deposition in the maxilla and mandible. Immunofluorescence shown that cell proliferation, apoptosis and osteoblast differentiation were impaired in ADAM10 cKO mice. Conclusion ADAM10, widely expressed in osteogenesis area of mice, can affect the intramembranous bone formation by regulating cell proliferation, differentiation and apoptosis.
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    Effect of Salvia Miltiorrhiza on Proliferation and Differentiation of 3T3-L1 Cells
    LIN Huaian,YU Li,WANG Jian,ZHANG Bo,ZHENG Danning,ZHOU Jia
    2017, 13 (1):  17-20.  DOI: 10.3969/j.issn.1673-0364.2017.01.005
    Abstract ( 457 )  
    Objective To explore the effect of Salvia miltiorrhiza on proliferation and differentiation of 3T3-L1 cells, so as to provide evidence for further research and clinical application of fat transplantation. Methods The differentiating 3T3-L1 cells after adipogenic induction were incubated with different concentration of S.miltiorrhiza (0.6 g/L, 0.3 g/L, 0.15 g/L, 0.075 g/L, 0.04 g/L, 0.02 g/L). After the 3T3-L1 cells differentiated into mature adipocyte, the cell proliferation viability was determined by CCK-8 assay kit. The lipid droplets accumulation of adipocyte were observed by Oil Red O staining and Image Pro Plus 6.0. The adipogenesis was quantified by measuring lipid content using triglyceride GPO-POD assay kit. Results S. miltiorrhiza promoted a dose-and time-dependent increase in 3T3-L1 cell proliferation viability. The earlier the dosing time was, the better the cell proliferation viability would be; The higher the concentration was, the better the cell proliferation viability would be. Within the six S.miltiorrhiza concentrations confine, S.miltiorrhiza also promoted a dose-dependent increase in 3T3-L1 cell differentiation ability, enhanced the lipid droplets accumulation of 3T3-L1 adipocyte. Conclusion S.miltiorrhiza can promote the increase of adipocyte number via the proliferation and differentiation of 3T3-L1 cells.
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    Research of Medical Collagen-Hydroxyapatite Artificial Bone for Sellar Reconstruction
    HE Jiajia,GONG Zhibiao,ZHANG Jianning,ZHU Tao
    2017, 13 (1):  21-24.  DOI: 10.3969/j.issn.1673-0364.2017.01.006
    Abstract ( 431 )  
    Objective To explore the effects and application prospect of the medical collagen-hydroxyapatite artificial bone (CHA) for sellar reconstruction. Methods From December 2013 to December 2014, 80 patients with pituitary adenoma were treated by endoscopic endonasal transsphenoidal surgery. All the patients were divided into two groups randomly. The patients of experimental group were used with CHA for sellar reconstruction, while the control group were treated by the traditional method. All the patients were followed up for 3-6 months. The general conditions, complications and sellar reconstruction of the 2 groups were observed and compared. Results There were no significant differences of operation time, blood loss and total resection rate between the 2 groups (P>0.05). Significant difference of the nasal cerebrospinal leaks was observed in the 2 groups (P0.05). New bone formation was observed in experimental group according to the CT results, and the CT value increased to the normal. Compared with the control group, the difference was significant (P<0.05). Conclusion The endoscopic transsphenoidal surgery for pituitary tumors is widely applied with minimal invasiveness. But the cerebrospinal fluid leakage is still a common complication. Using CHA for sellar reconstruction can reduce the nasal cerebrospinal fluid leaks.
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    The Inhibition of iRGD-Exosomes-Doxorubicin on the Proliferation of Malignant Melanoma in Vitro
    ZHAO Lijuan,ZHENG Jianghong,LIU Xiangdong,MAO Guangyu,DENG Chenliang,YANG Songlin
    2017, 13 (1):  25-28.  DOI: 10.3969/j.issn.1673-0364.2017.01.007
    Abstract ( 526 )  
    Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin (iRGD-exo-dox) on the inhibition of A375 cell line. Methods The exosomes were extracted from the iRGD-transfected A375 cells. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to A375 cells. The ability of iRGD-exo-dox on inhibiting the proliferation of A375 cells was analyzed. Results The affinity of iRGD-exo-dox to A375 cells was higher than the blank-exo-dox. The proliferation of A375 cells was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of A375 cells by targeting function.
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    Small and Medium Dose of Dexamethasone on Prevention of Early Complications of Soft Tissue after Acute Trauma in Lower Extremity
    FENG Yu,ZHANG Rui,ZHANG Ling,XIN Weiwei
    2017, 13 (1):  29-33.  DOI: 10.3969/j.issn.1673-0364.2017.01.008
    Abstract ( 500 )  
    Objective To investigate the clinical effect of small and medium dose of dexamethasone on prevention of early complications of soft tissue after acute trauma in lower extremity. Methods From January 2015 to March 2016, 350 patients with acute trauma in lower extremity were selected by using simple sampling method. All the patients were divided into 3 groups through a simple randomization, 116 cases in the control group, 116 cases in the small dose group, 117 cases in the medium dose group. All patients were treated with basic treatment, then the control group were treated with glucose infusion by intravenous injection, small dose group were treated with dexamethasone (0.1 mg/Kg) infusion by intravenous injection, medium dose group were treated with dexamethasone (0.2 mg/Kg) infusion by intravenous injection. The difference of limb blister incidence, limb circumference ratio, IL-6, CRP, osteofascial compartment syndrome occurred rate and adverse reaction rate among the three groups were compared. Results Limb circumference ratio of small dose group and medium dose group at 48 h were significantly less than that before treatment (P0.05). The level of CRP and IL-6 at 48 h in small dose group and medium dose group were significantly lower than that before treatment (P0.05). The adverse reaction among the three groups had no statistical significance ( P>0.05). Conclusion The rate of early complications of soft tissue after acute trauma of lower extremity can be effectively reduced by application of small and medium dose of dexamethasone. However, application of medium dose of dexamethasone can not increase the patient's clinical effect.
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    A New Measurement of Orbital Rim Area and its Primary Application in NF1
    GUO Chengrui,HU Xiaojie,LIN Xiaoxi,CHEN Hui,CHEN Xiaojun,QIU Yajing
    2017, 13 (1):  34-35.  DOI: 10.3969/j.issn.1673-0364.2017.01.009
    Abstract ( 505 )  
    Objective To explore a more accurate method in the area measurement of curved surface such as orbital rim. Methods Twenty-six NF1 patients were acquired. CT scan on both sides of the orbit and 3D reconstruction were completed. The orbital rim area was measured in approximate coronal plane and 3D curved plane respectively. The data were statistically analyzed. Results On average, the data of the plane method were underestimated, compared with the 3D method. There was statistical difference between the two groups. Conclusion The measurement in 3D curved plane is accurate, stable and easy to operate, which can replace the measurement in approximate coronal plane.
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    The Application of Nasolabial Fold Flap and Auricle Compound Tissue in Repairing Nasal Alar Defect
    HOU Junjie,CHEN Fengchao,YANG Xin
    2017, 13 (1):  36-39.  DOI: 10.3969/j.issn.1673-0364.2017.01.010
    Abstract ( 371 )  
    Objective To explore the application of nasolabial fold flap and auricle compound tissue in repairing nasal alar defect. Methods From October 2011 to March 2016, 32 patients with nasal alar defect were treated with nasolabial fold flap graft and auricle compound tissue free graft. The defect state of nasal alar, repair methods, incision scar, symmetry and postoperative appearance were all observed and analyzed. Results Nineteen patients received the treatment of nasolabial fold flap graft and 13 patients received the treatment of auricle compound tissue free graft. Nasal alar defect was well-repaired, and the outcome appearance was satisfactory without obvious scars around wound surface and donor tissue's incision. Conclusion Both nasolabial fold flap graft and auricle compound tissue free graft are favorable treatments for repairing nasal alar defect. Auricle compound tissue free graft should first be considered for patients without severe nasal alar defect, while nasolabial fold flap free graft should be employed for patients with severe nasal alar defect; Both methods have its advantages and disadvantages, the choice should be made base on the comprehensive consideration of the actual conditions of each patient.
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    Clinical Observation of the Combined Double Shuttle Like Incision in the Prevention of Lower Eyelid Ectropion after Blepharoplasty
    LI Zhen,XU Qing
    2017, 13 (1):  40-56.  DOI: 10.3969/j.issn.1673-0364.2017.01.011
    Abstract ( 388 )  
    Objective To explore a modified incision to solve the separation and lower eyelid ectropion after traditional blepharoplasty. Methods From June 2008 to October 2016, 338 cases received blepharoplasty were retrospectively analyzed. The combined double shuttle like incision (n=143) and traditional incision (n=195) were both used to correct the lower eyelid laxity. The postoperative complications were compared. Results The lower eyelid laxity was corrected in both two groups. No lower eyelid ectropion and separation were observed in the combined double shuttle like incision group, while the incidence of lower eyelid ectropion and separation in the traditional incision group were 1.03% and 4.87% respectively. There was significant difference between the two groups (P<0.05). Conclusion The combined double shuttle like incision can reduce the incidence of separation and lower eyelid ectropion. It is safe and reliable, and is worthy of clinical application.
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    Review
    Monitoring of Scaffold Degradation in Tissue Engineering
    WANG Xiangsheng
    2017, 13 (1):  42-44.  DOI: 10.3969/j.issn.1673-0364.2017.01.012
    Abstract ( 443 )  
    Biodegradable scaffolds as one of the three main factors in tissue engineering, play an important role in tissue regeneration. An ideal scaffold material must have appropriate degradation rate that could match the forming rate of tissue regeneration process. However, how to monitor the degradation of scaffold materials has been a great challenge in this field. In this paper, the latest literature about monitoring of scaffold degradation in tissue engineering was reviewed. The principle of each method as well as their advantages and disadvantages were summarized and the potential methods were discussed.
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    The Effect of Axonal Signal Neuregulin 1 on the Development and Regeneration of Schwann Cells
    ZHENG Yani
    2017, 13 (1):  45-47.  DOI: 10.3969/j.issn.1673-0364.2017.01.013
    Abstract ( 470 )  
    Physiological interactions and reciprocal signaling between axons and Schwann Cells (SC) play important roles in nerve development and regeneration after peripheral nerve injury. During development, axonal signaling is essential for SC proliferation, migration, differentiation and myelination. After nerve injury, Wallerian degeneration completely disrupts axon-SC contacts, SC genetic and phenotypic changes, cell proliferate and promote axon regeneration. When the regenerated axon contacts with denervated SC, the second proliferation wave begins. Studies have shown that neuronal axons through Neuregulin 1 (NRG1) and its erbB receptor to mediate the SCs proliferation activity, application of exogenous NRG1 can save SC and prevent apoptosis, promote SC proliferation and migration and axon regeneration. In this paper, NRG1 as a driving force for SC proliferation, differentiation, migration, myelination during development and redifferentiation, remyelination after injury were reviewed.
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    Research Progress of Choke Vessels Dilation
    MENG Xuchang,ZAN Tao,LI Qingfeng
    2017, 13 (1):  48-50.  DOI: 10.3969/j.issn.1673-0364.2017.01.014
    Abstract ( 452 )  
    The perforator flap has become a research focus in plastic and reconstructive surgery, and choke vessels is the emphasis and difficulty in the study of perforator flap. In this paper, the recently published literature at home and abroad about the choke vessels dilation was reviewed and summarized. The research of choke vessels is mainly about the morphology, and chemical activation of TRPV4 as a therapeutic target may promote the dilation of choke vessels.
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    The Etiology Research Progress of Hemifacial Microsomia
    ZHANG Zhuo,WANG Chuan,CHEN Junbao
    2017, 13 (1):  51-53.  DOI: 10.3969/j.issn.1673-0364.2017.01.015
    Abstract ( 472 )  
    Hemifacial microsomia (HFM) is the most common congenital facial anomaly except cleft lip and palate, which involves maldevelopment of craniofacial bones, muscles, soft tissues, facial nerves, auricles and extracranial organs. The disease affects unilaterally more frequently than bilaterally. The exact etiology and pathogenesis are still unclear. The reported pathogenesis includes genetics, environment and the mutual reaction. In this paper, the etiological investigations of HFM were classified and reviewed.
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    Causes and Therapies of Fistula after Phallic Reconstruction
    ZHANG Yan
    2017, 13 (1):  54-56.  DOI: 10.3969/j.issn.1673-0364.2017.01.016
    Abstract ( 473 )  
    One of the primary goals of the phalloplasty for female-to-male transsexuals is to gain the voiding ability in the standing position. However, fistula is one of the main complications. Prevention is the key to decrease the fistula. With the advance in modern medicine and science technology, the treatment of urinary fistula will be improved, for example, the development of tissue engineering provides a new pathway and opens up a wide prospect. In this paper, the causes and therapies of fistula after phallic reconstruction were reviewed.
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    Patient -Reported Outcome and Its Prospective Application in Plastic Surgery
    PAN Sida
    2017, 13 (1):  57-60.  DOI: 10.3969/j.issn.1673-0364.2017.01.017
    Abstract ( 403 )  
    Patient -reported outcome (PRO) has become a trend in modern medicine. Through psychometric measurement, PRO can demonstrate quantified information about the severity of disease and its affect on the patient 's functional state and health related quality of life, and thus provide substantial evidence on the efficacy of treatment. Standardized patient-reported outcome measurement may help with every aspect in the practice of plastic surgery. With PRO, the center of care can be transferred from the disease to the patient, which is exactly the goal of plastic surgery. In this paper, patient-reported outcome and its prospective application in plastic surgery were reviewed.
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