Journal of Tissue Engineering and Reconstructive Surgery ›› 2018, Vol. 14 ›› Issue (3): 123-126.doi: 10.3969/j.issn.1673-0364.2018.03.002

• Original article • Previous Articles     Next Articles

A Study of Constructing Tubular Tissue Engineered Cartilage by Using Chondrocytes Cell-Sheet Approach

LIU Yi,LI Dan,YIN Zongqi,ZHOU Guangdong,CAO Yilin   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College; Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering;National Tissue Engineering Center of China
  • Received:2018-03-22 Published:2020-07-23
  • Contact: 国家自然科学基金(31400830，81270070，81371703，81371701)；协和青年基金项目(3332014047)；上海市产学研医项目(15DZ1941600)

Abstract: Objective To explore the feasibility of constructing tubular cartilage with a diameter similar to goat tracheal in vitro by large mammalian auricular cartilage,based on improved cell-sheet technology.Methods The chondrocyte cell-sheet was cultured for 1-2 weeks in vitro,and was shaped as tubular cartilage tissue.Samples were collected at 6 weeks.The general appearance and elasticity were observed,and the formation of cartilage was examined.Results After cultured for 6 weeks in vitro,the cell-sheet had intergraded to a white translucent tubular tissue.The tubular tissue had certain biomechanical strength similar to mature cartilage tissues,and the histology analysis displayed continuous cartilage phenotype.Conclusion The improved cartilage cell-sheet technology has a series of advantage including limited defect in donor site,short period in vitro culture,less inflammatory response in vivo and so on.This technology would provide a promising approach for the regeneration of cartilage in vivo.

Key words: Tissue engineered cartilage, Cell-sheet technology, Tubular cartilage

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