Journal of Tissue Engineering and Reconstructive Surgery ›› 2011, Vol. 7 ›› Issue (1): 1-5.doi: 10.3969/j.issn.1673-0364.2011.01.001

• Original article •     Next Articles

Purification of Vascular Cells with MACS and FACS for Constructing Vessel from Differentiated Mouse Embryonic Stem Cells

YUE Wei1,2,CAO Yilin1,ZHANG Wenjie1   

  1. 1 Department of Plastic and Reconstructive Surgery,Shanghai Ninth People′s Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200011,China;2 Time Plastic Surgery Hospital,Shanghai 200021,China.
  • Published:2020-07-23
  • Contact: 国家重点基础研究发展计划　　（2005CB522700,2007CB948004,2011CB964704,2010CB944804）；国家自然科学基金　　（30571925,30671051）；上海市曙光人才计划　　（06SG22）

Abstract: Objective To compare the efficiency between using FACS and MACS for purification of smooth muscle cells and endothelial cells induced from differentiated mouse embryonic stem cells.Methods FLK-1+ cells were sorted from EBs by FACS and MACS,and were subsequently induced to vascular endothelial cells and smooth muscle cells with PDGF and VEGF respectively.Cells were then characterized by immunefluoresence staining and FACS.Results Large amount of vascular smooth muscle cells could be obtained from FLK-1 positive cells sorted by MACS.Over 90% of these cells were positive for smooth muscle markers,including SM α-actin,SM-SMC and Calponin.However,low output of smooth cells was observed by FACS sorting.Nearly 60% of cells expressed endothelial cell markers CD31 and vWF after VEGF induction of sorted cells by FACS,but low purity of endothelial cells was achieved from MACS sorting.Conclusion Large quantity of vascular smooth muscle cells with high purity can be obtained by MACS selection of FLK-1+ cells from EBs while high quality of vascular endothelial cells could be achieved by FACS sorting.

Key words: MACS, FACS, Purification, Embryonic Stem Cells, Tissue Engineering

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