Abstract: Objective To explore the effect of adipose stromal vascular fraction （SVF） cells on the LPL expression after fat transplantation. Methods Freshly isolated SVFs （experimental group） were mixed with adipose tissue and then injected under the back skin of nudes randomly on one side. In the same manner, the mixture of physiological saline and adipose tissue （control group） were injected on the other side. Immunohistochemistry for perilipin, immunohistochemistry for LPL and western blot for LPL were combined to explore the trends of LPL expression at 1, 2, 3 and 4 weeks. Results According to the results of western blot and immunohistology of LPL, the levels of LPL started to decrease as early as the transplantation in both groups. And the expression of LPL in the experimental group was higher than the control group at 2, 3 and 4 weeks after implantation. Immunohistology of perilipin indicated no remarkable changes in the viable adipocyte zone during the first week. The adipose cells decreased day to day in both group. And 2, 3 and 4 weeks after implantation there were more survived adipose cell in the experimental group. Conclusion SVF can improve the expression of LPL in transplanted adipose tissue. It begins to make effects at the second week post-implantation. It may be related to its effect on the adipose cell survival and the adipogenic differentiation of ASCs.

Key words: Adipose-derived stromal ceils, Fat transplantation, Lipoprotein lipase, Perilipin