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Table of Content

    23 July 2020, Volume 11 Issue 3 Previous Issue    Next Issue
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    Original article
    Layer-by-layer Self-assembly Coating in the Construction of Sustained Release System on Root Surface
    ZHANG Hong,CEN Lian,WANG Jun
    2015, 11 (3):  121-123.  DOI: 10.3969/j.issn.1673-0364.2015.03.001
    Abstract ( 442 )  
    Objective To construct a sustained release system of exogenous protein on the root surface using layer-by layer self-assembly coating technique. Methods Eighteen healthy mandibular second premolars were collected and randomly divided into 6 groups, group a-e were experimental groups with acid etching, group f was a control group without etching. Each group went through layer-by-layer adsorption experiments under different conditions. Samples were collected and detected absorbance on day 1-9 in each group to calculate the concentration of protein release. Results All sets of samples showed sustained-release trend (P〈0.05). Etching group showed a significant increase of protein release compared with control group (P〈O.05); Release rate and amount increased with the protein concentration in the materials (P〈0.05); More adsorption times leaded to larger release amount and prolonged time (P〈0.05). Conclusion Sustained release system of exogenous protein can be constructed on the root surface using layer-by layer self-assembly coating technique.
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    The Biosecurity of the High-Performance Biomedical Magnesium Alloy (JDBM) Screws
    KONG Xiangdong,HAO Yongqiang,WANG Lei,MAO Zhenyang,PAN Wei,DAI Kerong,YUAN Guangyin
    2015, 11 (3):  124-127.  DOI: 10.3969/j.issn.1673-0364.2015.03.002
    Abstract ( 693 )  
    Objective To explore the safety of the new high-performance biomedical magnesium alloy (JDBM) in vivo and to evaluate its influence on functions of organs and local response. Methods New Zealand rabbits were used to build the JDBM magnesium alloy implantation model. Regular observation of wound healing and general condition was firstly carried out. And then blood specimen was collected to detect the blood routine, coagulation function, hepatic and renal functions, cardiac function and the content of calcium, phosphate and magnesium. The heart, liver, spleen, lung, kidney and muscle tissues around the screw were collected for HE staining to observe pathological changes. X-ray was carried out to observe the changes in bone density around the screw, and the osteogenesis property of screw was preliminarily analyzed. Results This new material had no effect on the results of blood routine index, coagulation function, hepatic function, renal function and cardiac function. NO pathological changes of organs were observed, and no inflammatory reaction of foreign body was detected in musculi parenchyma as well by histological detection. X-rays showed no significant gas accumulated around the screw and the screw degraded slowly. The screw was clearly visible six months after operation, and an apparent thickening was found near the bone cortex which hinted a relatively strong bone formation. Conclusion JDBM is a new kind of magnesium alloy material with good biosecurity.
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    Role of Sulf2 in Promoting Lymphangiogenesis
    ZHU Chenfang,NIE Xin,HE Liu,YANG Zhi,GU Yan
    2015, 11 (3):  128-132.  DOI: 10.3969/j.issn.1673-0364.2015.03.003
    Abstract ( 484 )  
    Objective To explore the role of Sulfatase2 (Sulf2) in promoting lymphangiogenesis. Methods According to Sulf2 cDNA sequence, pCDH-Flag-Sulf2 eukaryotic expression vector was designed and constructed, then transfected into 293FF cell line to obtain and purify rSulf2. The functions of LECs were examined and compared with control group by flow cytometry, matrigel assay and mice ear lymphangiogenesis model to figure out the effects of rSulf2 on cell cycles, apoptosis, tube formation ability and lymphangiogenesis in LECs. Results The construction of pCDH-Flag-Sulf2 vector was proved correctly by restriction enzyme digestion and sequencing test. Sulf2 expression increased in 293FF cells showed by Western blot. Purified Sulf2 reduced no serum medium induced apoptosis and increased the number of living cells, but it had no effect on cell cycles in LECs. Sulf2 also promoted the tube formation ability in vitro and the number of lymphatic vessels in nude mice ear. Conclusion Sulf2 can inhibit LECs apoptosis and promote lymphangiogenesis.
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    Hemodynamic Studies of The Venous Augmented DIEP Flap in Pig
    XIN Minqiang,LUAN Jie,MU Dali
    2015, 11 (3):  133-135.  DOI: 10.3969/j.issn.1673-0364.2015.03.004
    Abstract ( 368 )  
    Objective To evaluate the efficacy of venous augmentation of DIEP flap in pig and to clarify the hemodynamics. Methods Twenty four female Yorkshire micro-pigs were included. All of the pigs were performed DIEP flap surgery to form DIEP flap and venous augmented DIEP flap, then several hemodynamic indexes between the two different flap types were evaluated and compared, including flap perfusion, venous pressure and venous blood gas analysis. After the analysis of the dynamics, all of the flaps were sutured up, while 12 of the flaps formed venous augmented DIEP flap (experimental group) and the rest 12 pigs formed classic DIEP flap (control group). The animals were sacrificed at the 7th postoperative day, and the final partial necrosis rate and the survival rate were compared between the two groups. Results Compared to the condition in none venous augmentation, all the dynamic indexes were improved dramatically after the application of venous augmentation. The flap survival rate was (99.4±1.2)% in the experimental group, and (82.1±7.6)% in the control group. There was significant difference between the two groups. Conclusion The venous augmentation technique is effective in promoting the blood perfusion and elevating the viability of DIEP flap. Venous augmentation is a reliable technique to improve flap venous congestion and viability, especially for those large flaps.
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    Pattern Study of Needle-free Injector in Treating Keloid
    LIU Yang,HUANG Ruozhen,CHEN Xiangdong,SUN Qilin,CHEN Jun
    2015, 11 (3):  136-138.  DOI: 10.3969/j.issn.1673-0364.2015.03.005
    Abstract ( 469 )  
    Objective To explore the model of drug penetration using low-pressure needle-free injector in treating keloid. Methods The drug was injected into the dorsal skin of fragrance pigs and in vitro keloid tissues with needle-free injector. The degree of diffusion and depth under different pressures and dosages were observed, and the pattern of the drug diffusion was also inspected. Results The degree of dispersion and depth were increased with the dosage under the same pressure, and the two values also showed a rise trend with the increase of the pressure in the same dosage. Using the traditional needle injection, the drug showed a nodular distribution in the keloid tissues. However, the drug was distributed in the superficial layer of the tissues under 150 psi using needle-free injector, "T" shape under 175 psi and "I" shape under 200 psi. Conclusion Under 150 psi and 175 psi, the drug can be injected into the superficial dermis and all the layers of keloid respectively by needle-free injector, while under 200 psi, it is appropriate for the treatment of the deeper and more compact keloid.
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    The Inhibition Effect of Intra-cicatrix Injection of ADSCs on Ear Hypertrophic Scar of Rabbit
    ZHANG QI,LIU Lina,DENG Jingcheng,CAO Weigang
    2015, 11 (3):  139-143.  DOI: 10.3969/j.issn.1673-0364.2015.03.006
    Abstract ( 434 )  
    Objective To investigate the inhibition effect and mechanism of intra-cicatrix injection of adipose derived stem cells on hypertrophic scar in rabbit ear. Methods Twelve New Zealand white rabbits were used for establishing scar model and were randomly divided into 3 groups. Two weeks after the operation, all the right ears were injected DMEM as internal control while left ears of group 1, 2 were injected ADSCs, ADSCs-CM respectively. Left ears in the third group were remained untouched. Photos and ultrasonography were taken before and 1, 2, 3 weeks after injection. Histochemical and genetic detection were used 3 weeks after the rabbits were sacrificed for offering scar tissue. Results All wounds were re- epithelized 2 weeks after the injection. Wounds injected DMEM and untouched gradually grew thick, red and stiff which are the symptoms of hypertrophic scars, while ADSCs and ADSCs-CM injected ones showed no sign of growing hypertrophic. HE and Masson's staining showed collagen deposit and irregularly arrangement in the DMEM injection and untouched scars, while much less and better arranged collagen deposit were shown in both ADSCs and ADSCs-CM treated ones. Genetic detection showed lower expression of α-SMA, Collagen I in ADSCs and ADSCs-CM injection scars, compared with DMEM treated and untreated ones. Dil label staining showed a larger amount of ADSCs in the scar tissue of ADSCs treated group. Conclusion Intra-cicatrix injection of ADSCs can inhibit hypertrophic scar through ameliorating collagen deposit by down regulate the expression of α-SMA, Collagen I.
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    The Effects of Salvia miltiorrhiza and Astragalus propinquus Injection on the Survival of Autologous Adipose Tissue Transplantation
    LI Pengfei,ZHENG Danning,ZHOU Jia,WANG Jian,ZHANG Bo,ZHU Chang,YU Li
    2015, 11 (3):  144-162.  DOI: 10.3969/j.issn.1673-0364.2015.03.007
    Abstract ( 341 )  
    Objective To explore the effects of Salvia mihiorrhiza and Astragalus propinquus injection on the survival of transplanted grafts, so as to provide evidence for further research and clinical application. Methods Autologous adipose tissue transplantation model was established using New Zealand white rabbit. The experimental groups were given S. miltiorrhiza and A. propinquus injection intraperitoneally. The expression levels of VEGF in serum and survival rates were measured and calculated at different time points. Tissue morphology was observed by HE staining. The expression of platelet endothelial cell adhesion molecule-1 (CD31) and perilipin were observed by immune-histochemistry to evaluate the neocapillary growth and cyto-activity of transplanted adipose tissue. Results Twelve weeks after transplantation, the survival rates in experimental groups were higher than the control group. The expression levels of VEGF in serum in experimental groups were significantly higher than those in the control group at each time point. HE and immuno-histochemical staining demonstrated better morphology, denser neo-capillaries and higher cyto-activity in experimental groups, compared with the control group. Conclusion By applying S. mihiorrhiza and A. propinquus injection, the survival of autologous transplanted adipose tissue can be significantly enhanced in New Zealand rabbits.
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    Effect of SVF Cells on the LPL Expression after Fat Transplantation
    FAN Qi,ZHENG Danning,YU Li,WANG Jian,LI Pengfei
    2015, 11 (3):  148-151.  DOI: 10.3969/j.issn.1673-0364.2015.03.008
    Abstract ( 360 )  
    Objective To explore the effect of adipose stromal vascular fraction (SVF) cells on the LPL expression after fat transplantation. Methods Freshly isolated SVFs (experimental group) were mixed with adipose tissue and then injected under the back skin of nudes randomly on one side. In the same manner, the mixture of physiological saline and adipose tissue (control group) were injected on the other side. Immunohistochemistry for perilipin, immunohistochemistry for LPL and western blot for LPL were combined to explore the trends of LPL expression at 1, 2, 3 and 4 weeks. Results According to the results of western blot and immunohistology of LPL, the levels of LPL started to decrease as early as the transplantation in both groups. And the expression of LPL in the experimental group was higher than the control group at 2, 3 and 4 weeks after implantation. Immunohistology of perilipin indicated no remarkable changes in the viable adipocyte zone during the first week. The adipose cells decreased day to day in both group. And 2, 3 and 4 weeks after implantation there were more survived adipose cell in the experimental group. Conclusion SVF can improve the expression of LPL in transplanted adipose tissue. It begins to make effects at the second week post-implantation. It may be related to its effect on the adipose cell survival and the adipogenic differentiation of ASCs.
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    Emergency Management of Total Scalp Avulsion with Microsurgical Replantation
    ZHU Xuefeng,DENG Jingcheng,JIANG zhaohua,CAO Weigang,LI shengli,Cheng Kaixiang
    2015, 11 (3):  152-155.  DOI: 10.3969/j.issn.1673-0364.2015.03.009
    Abstract ( 430 )  
    Objective To explore the emergency management of total scalp avulsion with microsurgical replantation. Methods From July 2009 to December 2014, 6 cases with total scalp avulsion were treated with microsurgical replantation. Initial management in the emergency department included correction of hemorrhagic shock with early blood transfusion, intravenous rehydration and wound compression after rapid physical examination. A compound trauma and preoperative workup prior to replantation was performed to exclude any associated life-threatening injuries. Results The scalp of 6 cases was survived well, good appearance and function of the completely avulsed tissues and organs were achieved in all the cases. Conclusion Successful replantation can achieve the best esthetic and functional results when compared with other procedures. In addition, preoperative evaluation and preparation in the emergency department are considered to be an important part of the successful salvage of the avulsed scalp.
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    Early Surgical Treatment by Intracranial Approach in Treating Traumatic Optic Neuropathy Caused by Cranial-orbito-zygomatic Complex Fractures
    CHENG Zhihua,LUO Cong,GUO Yu,GUO Zhilin
    2015, 11 (3):  156-165.  DOI: 10.3969/j.issn.1673-0364.2015.03.010
    Abstract ( 335 )  
    Objective To explore the effect of early intracranial optic nerve decompression and internal fixation in treating cranial-orbito-zygomatic complex fractures with traumatic optic neuropathy (TON). Methods From January 2011 to January 2013, 15 patients with TON caused by cranial-orbito-zygomatic complex fractures were retrospectively analyzed. Visual recovery were observed and recorded before and after optic nerve decompression. Results The periods between the injury and operation were ranged from 8 to 45 hours. All patients had optic canal fractures and visual loss before surgery. All the patients were followed up for 6 months. The visual acuity improvement was achieved in 10 patients after optic nerve decompression. Conclusion Early single-stage repair of orbitozygomatic complex fractures and intracranial optic nerve decompression are effective and safe for patients with traumatic optic neuropathy.
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    Changes of Temporomandibular Joint after Sagittal Split Ramus Osteotomy Combined with Post-operative Orthodontic in Mandibular Prognathism Patients
    YANG Liya, TENG Li, SUN Xiaomei, XU Jiajie, LU Jianjian, ZHANG Chao, XIE Fang, XU Meibang
    2015, 11 (3):  158-162.  DOI: 10.3969/j.issn.1673-0364.2015.03.011
    Abstract ( 475 )  
    Objective To explore the change of the TMJ after bilateral sagittal split ramus osteotomy (BSSRO)- postoperative orthodontics therapy by radiographic measurement in mandibular prognathism (MP) patients. Methods From 2012 to 2014, 24 patients (8 male and 16 female) diagnosed with MP with and without asymmetry were included in this study. They were divided into 2 groups (12 symmetric patients and 12 asymmetric patients) and all received SSRO - postoperative orthodontics therapy. TMJ space, condylar and ramus angle were assessed by computed tomography (CT) pre- and post- operatively. Results There was no significant difference on the deviation side between the asymmetry and symmetry groups. Coronal ramus angle on the non - deviation side in the symmetry group was significantly larger than that in the asymmetry group (P=0.016 1). Horizontal Condylar angle on the deviation side in the symmetry group was significantly smaller than that in the asymmetry group while no significant difference was found on the non - deviation side between the asymmetry and symmetry groups postoperatively (P=0.017 9). The postoperative coronal condylar angle and anterior joint space were significantly larger than the preoperative value on non - deviation side in symmetry group (P=-0.035 5 and 0.041 2, respectively). The oostoperative coronal ramus angle and saggital ramus angle were larger while the sunerior Joint soace was smaller than the preoperative value on non - deviation side in asymmetry group (P=0.017 5, 0.039 8 and 0.031 9, respectively). The preoperative condylar position was not changed on deviation side in either group. Conclusion Significant expansion of coronal condylar angle and anterior joint space could occur on the non - deviation side in symmetry group. In asymmetry group, the coronal ramus angle and saggital ramus angle.can be enlarged and the superior joint space can be reduced.
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    Application of Mohs Micrographic Surgery in the Treatment of Nasal Basal Cell Carcinoma
    CHEN Jun,SUN Qilin,LIU Yang,CHEN Xiangdong
    2015, 11 (3):  163-165.  DOI: 10.3969/j.issn.1673-0364.2015.03.012
    Abstract ( 357 )  
    Objective To evaluate the effect of Mohs micrographic surgery in nasal basal cell carcinoma. Methods In 2014, 40 cases with nasal basal cell carcinoma were treated by Mohs micrographic surgery. The surgical effects were observed and compared with the traditional extended resection. Results The mean margin in this group using Mobs micrographic surgery was 1.8 mm. Operative incision of 39 cases were primary healing. Patients were satisfied with the appearance and surgical effects. All the patients were followed up for 6 to 24 months, no recurrence was observed. Conclusion Mobs micrographic surgery is more complete than traditional extended resection for treating nasal basal cell carcinoma. It can make more solutions to repair the lesions.
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    The Application of 3-Dimensional Rib Cartilage Computed Tomography in Adult Auricular Reconstruction for Microtia
    LI Chongzhao, ZHOU XU, ZHANG Qingguo, XIE Yangchun, LIU Dun, QIAN Jin, CHEN Weiwei, CHENG Lin
    2015, 11 (3):  166-181.  DOI: 10.3969/j.issn.1673-0364.2015.03.013
    Abstract ( 481 )  
    Objective To study the feasibility of 3-dimensional rib cartilage CT measurement on guiding rib cartilage harvest of adult patients with congenital microtia for auricular reconstruction. Methods From Janual7 2014 to December 2014, 46 adult patients suffering congenital microtia were included in this study. The measurements of 3D CT reconstruction on the sixth, seventh, eighth rib cartilage were finished before surgery. The amounts of rib cartilage for carving ear cartilage scaffold were determined by the measurements. All patients were followed up for 4 months to 1 year. Results There were 20 cases (43.48%) with bilateral asymmetry of the sixth, seventh, eighth rib cartilage. Meanwhile, there were 14 patients (30.43%) with significantly calcified rib cartilages. One case (2.17%) suffered rib cartilage fracture during harvesting rib cartilage. And mild chest deformity was observed in 2 cases (4.35%) after surgery. Conclusion Harvest of rib cartilage for adult ear reconstruction can be promoted by preoperative measurements of 3D rib cartilage morphology. Meanwhile, postoperative complications could be decreased.
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    The Sensation Recovery in Reconstructed Phallus by Cheng's Method
    LIU Yang,CHENG Kaixiang,CHEN Fuguo,CHENG Chen,MA Sunxiang
    2015, 11 (3):  169-171.  DOI: 10.3969/j.issn.1673-0364.2015.03.014
    Abstract ( 540 )  
    Objective To explore the sensation recovery of the reconstructed penis by Cheng's method. Methods A long term follow-up of 27 patients undergoing penile reconstruction by Cheng's method were performed. Sensory testing including tactile sensation and static two-point discrimination was performed in a standardized pattern. Results The tactile sensation of the reconstructed glans began to recover 6 months after phalloplasty. The static two-point discrimination began to recover 6-12 months after operation and reach stability to 10-14 mm 2-3 years after operation. The tactile sensation of the reconstructed penile body began to recover 6 months after phalloplasty. The static two-point discrimination reached 15- 20 mm 6-12 months after operation. Conclusion The sensation recovery in reconstructed phallus by Cheng's method begin to recover 6 months after operation and reach stability after 2-3 years.
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    Pedicled Transplants of Sigmoid Colon for Vaginal Reconstruction
    MA Sunxiang, CHENG kaixiang, CHENG Chen,CHEN Fuguo, LIU Yan, CHEN Yah, SUN Hua LIU Jianhua
    2015, 11 (3):  172-173.  DOI: 10.3969/j.issn.1673-0364.2015.03.015
    Abstract ( 424 )  
    Objective To explore the technique of vagina reconstruction using pedicled transplants of sigmoid colon and its effect. Methods From February 2009 to September 2014, 13 female patients with congenital absence of vagina were treated with this technique in our hospital. The physical examination, questionnaire of satisfaction were performed after surgery. Results The operations were successfully completed in all the 13 cases. The appearance of cunnus was fine, the neovaginal length was 10-12 em, and the diameter was about 3 cm. The reconstructed vaginal wall was soft, moist and elastic. All the patients were followed up for 8 to 75 months. Ten patients had sexual life after operation. All the patients were satisfied with the results of the surgery. Conclusion The use of pedicled transplants of sigmoid colon for vaginal reconstruction is an effective method for female patients with congenital absence of vagina.
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    Biomechanical Analysis of Embedded Cross-stitch Suture and Its Application on Extensor Tendon Repair in Zone Ⅶ
    ZHOU Shengbo,MAO Hailei,YANG Xi,XU Qiming,WANG Yue,JIANG Yongkang,WANG Bin
    2015, 11 (3):  174-177.  DOI: 10.3969/j.issn.1673-0364.2015.03.016
    Abstract ( 556 )  
    Objective To investigate the biomechanical properties of embedded cross-stitch suture and its preliminary application for extensor tendon repair in zone Ⅶ. Methods Thirty-three fresh porcine extensor tendons were randomly divided into 3 groups and repaired with cross-stitch, embedded cross-stitch or Halsted suture method respectively. The tendons were subjected to an Instron tensile testing machine to test 2-mm gap formation force, ultimate strength, stiffness and energy to failure. Embedded cross-stitch was performed for repairing extensor tendons in zone V~ of 56 fingers. Protected passive and active motion protocol was used in each case after operation. Strickland TAM classification was applied to evaluate the function postoperatively. Results Under linear tension mode, embedded cross-stitch was superior to cross-stitch and Halsted method with its 2-mm gap formation force at (49.2±5.6) N, ultimate strength at (68.3±6.3) N, Stiffness at (6.9±0.7) N/mm, and energy to failure at (0.79±0.07) J (P〈0.05). Patients were followed up for 26 months, no tendon rupture was observed in any of the repaired fingers. Strickland TAM classification showed excellent in 50 fingers, good in 4 fingers, fair in 2 fingers, and the total good rate was 96.4%. Conclusion Embedded cross-stitch suture employs strong biomechanical characters with fewer suture exposure, which can fulfill the requirements for early motion protocol. Therefore, it is suggested for extensor tendon repair.
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    The Change of Volume Maintenance after Secondary Autologous Fat Graft for Breast Augmentation
    WANG Yirong,SUN Jingjing,JI Kai,MU Dali,XIN Minqiang,LUAN jie
    2015, 11 (3):  178-181.  DOI: 10.3969/j.issn.1673-0364.2015.03.017
    Abstract ( 351 )  
    Objective To compare the percentage volume maintenance after the first and second autologous fat graft, and to explore the change of percentage volume maintenance. Methods From March 2011 to December 2014, 9 patients received autologous fat graft for breast augmentation were included for this retrospective study. The percentage volume maintenance after the first fat graft session and overall percentage volume maintenance after the second fat graft session were calculated using three-dimensional photography. The t test was performed to compare the results. Results The mean percentage volume maintenance was 0.25 ±0.06 after the first fat graft session, while the overall mean percentage volume maintenance was 0.32±0.06 after the second fat graft session. The percentage volume maintenance after the second fat graft session was significantly increased (P=0.000 7). Conclusion There is a significant improvement of percentage volume maintenance after the second fat graft session.
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    Sclerotherapy in the Treatment of Skin Microcystic Lymphatic Malformations
    WANG Haizhen,DENG Jingcheng,JIANG Zhaohua
    2015, 11 (3):  182-192.  DOI: 10.3969/j.issn.1673-0364.2015.03.018
    Abstract ( 599 )  
    Objective To explore the effectiveness of local bleomycin injection in the treatment of microcystic lymphatic malformations (LMs). Methods From January 2009 to December 2013, 32 patients with microcystic LMs were treated by bleomycin injection and followed prospectively. Ultrasound examination were used to confirm the diagnosis and determine the scope and level of the cyst before bleomyein injection. Each patient received bleomyein injection at an interval of 4 to 6 weeks. Results The mean injection times was 3.5 times. Thirty cases were cured and 2 cases were partially cured. All the patients were followed up for 1-4 years. Ultrasound follow-up demonstrated no recurrence of microcystic LMs. And no drug side-effects were observed. Conclusion Local application of bleomyein in patients with microcystic LMs is a safe and effective method. Totally healing can be achieved in the majority of cases.
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    Clinical Efficacy of Compound Prescription of Traditional Chinese Medicine-Lymph Fang on the Treatment of Chronic Extremity Lymphedema
    LUO Yi,WANG Li,CHEN Jiajia,LIU Ningfei
    2015, 11 (3):  185-188.  DOI: 10.3969/j.issn.1673-0364.2015.03.019
    Abstract ( 722 )  
    Objective To investigate the effects of compound prescription of traditional Chinese medicine-lymph Fang on the treatment of chronic extremity lymphedema. Methods From September 2011 to December 2014, 239 patients with chronic extremity lymphedema were taken compound Chinese medical lymph Fang orally. The extent of tissue edema were detected by Multiple-frequency bioelectrical impedance analysis before and after treatment. The circumference of the extremities were measured before and after treatment. Results After treated for one course, remarkable reduction of accumulated edema fluid in tissue and circumference were observed (P〈0.05, P〈0.05). On long-term follow-up, most of the patients felt better. The erysipelas seizure of frequency were decreased significantly. The correlation between edema lessening and circumference reducing was obviously significant (r=0.738, P〈0.01). Conclusion Compound Chinese medical lymph Fang can effectively reduce the edema of extremities.
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    Effect of Ultrasound-guided EVLA in the Treatment of Klippel-Trenaunay syndrome (KTS): A Retrospective Study
    ZHAO Haiguang,LIU Xiaobing,SHI Huihua,YANG Xinrui,LU Xinwu
    2015, 11 (3):  189-192.  DOI: 10.3969/j.issn.1673-0364.2015.03.020
    Abstract ( 394 )  
    Objective To explore the effect of ultrasound-guided EVLA in the treatment of Klippel-Trenaunay syndrome (KTS). Methods A retrospective analysis were made for 47 KTS patients treated with EVLA from March 2007 to December 2010, and they were followed up for 0.5-5 years. Results After the EVLA and ligation treatment of varicose veins and KT veins for 2 weeks, blood flrw was not detected. After 6 months of treatment, blood flow of 45 cases (95%) was still closed. Local recurrence of varicose veins was observed in 2 cases. And after 1 year and later, local recurrence of varicose veins was observed in 9 cases. The superficial varicose veins and KT veins of other patients disappeared completely or only minor fibrous tissue was left, in which blood flow was not detected. Most of the treated veins became fibrous cords, and were hard to identify. No serious adverse reaction was observed. Conclusion Ultrasound-guided EVLA is a safe, simple and effective method for the treatment of varicose veins and KT veins in KTS patients.
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    Effect of Botulinum Toxin Type A in the Treatment of Masseteric Hypertrophy
    GAO Qiuni,DAI Chuanchang
    2015, 11 (3):  193-195.  DOI: 10.3969/j.issn.1673-0364.2015.03.021
    Abstract ( 335 )  
    Objective To evaluate the effect of botulinum toxin type Ain the treatment of masseteric hypertrophy.Methods From August 2005 to January 2014,15 women with masseteric hypertrophy were enrolled in this study.Fifty units of botulinum tosin type A was injected bilaterally in the masseter muscles .The thickness of masseter muscles was measured preoperatively,4,12 and 24 weeks postoperatively using B-ultrasound .Results All the patients were followed up for 6 months.The mean reduction of masseter muscle thickness were (15.60±4.96%)at 4 weeks and (27.72±9.13)%at 12 weeks postoperatively.The percent of reduction decreased to (24.32±8.86)%at 24 weeks postoperativelyl.Conclusion Botulinum toxin type Ais effective in treating hypertrophy of the masster muscle .B-ultrasound could effectively reflect the reduction of muscle thickness after botulinum toxin type A injection.
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    Evaluation of Narrow-spectrum Intense Pulsed Light for the Treatment of Burn Scar
    WANG Shen,JIN Rui,MI Jing,WANG Juan,DONG Jiying
    2015, 11 (3):  196-198.  DOI: 10.3969/j.issn.1673-0364.2015.03.022
    Abstract ( 526 )  
    Objective To evaluate the effectiveness and safety of narrow-spectrum intense pulsed light for the treatment of burn scar. Methods From May 2014 to February 2015, 37 cases with burn scar (48 places) were treated with narrow- spectrum intense pulsed light (DPL, 500-600 nm). The treatment parameter was set up according to the color of the scar and the immediate vessel responses. The treatment interval was 4-6 months. Results Nineteen scars had significant improvement, 26 scars had moderate improvement, and 3 scars had no improvement. The effective rate was 93.75%. Adverse effects included transient erythema, blister and transient PIH. Conclusion The narrow-spectrum intense pulsed light can be considered as an effective and safe method for the treatment of burn scar.
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    High-energy CO2 Fractional Laser and Fractional Radio Frequency for the Treatment of Atrophic Acne Scar: A Serf-control Study
    ZHU Yujie,LIU Yang,ZHANG Zhen,WU Pinru
    2015, 11 (3):  199-201.  DOI: 10.3969/j.issn.1673-0364.2015.03.023
    Abstract ( 510 )  
    Objective To evaluate the safety and effectiveness of CO2 fractional laser and fractional radio frequency for the treatment of atrophic acne scars. Methods Half face was randomly assigned to CO2 fractional laser with DeepFx mode or fractional radio frequency. The atrophic acne scars were firstly treated by pinpoint irradiations with one pass, the second scanning was performed for whole area. Three months after five treatment sessions with interval of 2 months, improvements of acne scars were scored using a quartile grading scale, and side effects were monitored at each follow-up visit. Results Twenty-eight patients completed the study. There was no significant difference of improvements on both sides of the acne scar. Hyperpigmentation was significantly higher in CO: fractional laser treatment side than fractional radiofrequency treatment side, the pain scores of CO2 fractional laser treatment side was less than the fractional radio frequency treatment side. No permanent hypopigmentation and scarring formation were observed. Conclusion Both CO2 fractional laser and fractional radio frequency can safely and effectively improve atrophic aene scars.
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    Effectiveness of Dexmedetomidine Combined with Remifentanil in Anesthesia for Liposuction
    ZHI Yankang,GAO Qiuni,LIU Chunfang,JIANG Hong
    2015, 11 (3):  202-204.  DOI: 10.3969/j.issn.1673-0364.2015.03.024
    Abstract ( 356 )  
    Objective To investigate the effectiveness of dexmedetomidine combined with remifentanil in the anesthesia for liposuction. Methods Sixty patients for liposuction were randomly divided into 3 groups (n=20): dexmedetomidine+ remifentanil group (group DF), dexmedetomidine group (group D) and local anesthesia group (group N). Group DF and group D were intravenously infused with 1 Ixg/Kg dexmedetomidine in ten minutes. Group DF received remifentanil 0.1 p,g/Kg.min in addition, When the Ramsay sedation score reached 3 points, operation started. The rate of dexmedetomidine was maintained 0.5 μg/(Kg .h) during the operation. No intravenous anesthetics was given in Group N. All groups were injected with tumescent fluid. Scoring criteria included Ramsay sedation score, VAS pain score and HR, MAP, SPO2 in different time point were all observed. And the adverse reactions were recorded. Results HR, MAP in group N were higher than in group DF and group D (P〈0.05). No difference of HR, MAP and SPO2 were observed between group DF and group D (P〉0,05). The VAS pain score in group DF and group D were both higher than in group N (P〈0.05). The VAS pain score in group DF was also better than in group D (P〈0.05). No serious adverse reactions were observed in all groups. Conclusion Dexmedetomidine combined with remifentanil used for liposuetion is effective, and is superior to single use of dexmedetomidine.
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    Review
    Application of Chitosan in Laryngeal Cartilage Tissue Engineering
    CUI Xiang,HOU Ruixiang,LI Qun,LU Dakai,DENG Hongxia,SHEN Zhiseng
    2015, 11 (3):  208-212.  DOI: 10.3969/j.issn.1673-0364.2015.03.026
    Abstract ( 393 )  
    The repair and reconstruction of laryngeal cartilage defects caused by laryngeal cancer, stenosis, trauma is a major challenge of the Otolaryngology. Due to the absence of blood vessel and lymph, the cartilage has poor ability to repair or regenerate itself once damaged. Little is reported on the enduring cartilaginous tissue to replace damaged laryngeal cartilages. The rise of tissue engineering offers new opportunities for laryngeal cartilage reconstruction. It is critical to develop appropriate scaffold materials to support cell adhesion, proliferation and neocartilage regeneration so as to repair laryngeal cartilage. Chitosan possesses excellent biocompatibility, the ability to induce chondrocyte adhesion, proliferation and differentiation, flexibility to process, and controllable biodegradation rate. It has been one of the most promising biomaterials for cartilage tissue engineering. However, little has been reported on the application of chitosan in the laryngeal cartilage tissue engineering. This review summarized the characteristics of laryngeal cartilage and updated chitosan-based materials for cartilage tissue engineering. Based on these, possible application of chitosan based materials for the laryngeal cartilage tissue engineering was discussed.
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    Research Progress of Dental Pulp Stem Cells for Nervous Tissue Reconstruction
    MIAO Zhe
    2015, 11 (3):  213-215.  DOI: 10.3969/j.issn.1673-0364.2015.03.027
    Abstract ( 437 )  
    Dental pulp stern cells (DPSCs), as adult stem cells, have been reported for laboratory application on nervous tissue reconstruction. Hereby, in this article, characteristics of DPSCs and neural differentiation, scaffolds research for nervous tissue repair, experimental application of DPSCs on nervous tissue reconstruction and relevant bio-mechanisms were reviewed.
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