阿尔茨海默病患者外周血淋巴细胞中G蛋白偶联受体激酶5水平与疾病及程度间相关性探讨

doi:10.16150/j.1671-2870.2018.04.012

诊断学理论与实践 ›› 2018, Vol. 17 ›› Issue (04): 419-422.doi: 10.16150/j.1671-2870.2018.04.012

阿尔茨海默病患者外周血淋巴细胞中G蛋白偶联受体激酶5水平与疾病及程度间相关性探讨

孙家兰, 黄澍, 来小音, 朱玮, 胡荣郭, 李龙宣

上海市浦东新区公利医院神经内科,上海 200135

收稿日期:2018-06-11 出版日期:2018-08-25 发布日期:2018-08-25
通讯作者: 李龙宣 E-mail: Longxuanlee2006@yahoo.com
基金资助:
上海市卫生和计划生育委员会科研资金（201540157）; 上海市浦东新区卫生系统领先人才培养计划（PWR12014-05）; 上海市医学重点专科建设计划项目（ZK2015B16）; 上海市卫生计生系统优秀学科带头人培养计划（2017BR051）; 国家自然科学基金（81771328）

Correlation between lymphocyte G-protein-coupled receptor kinase-5 level and cognitive impairment in patients with Alzheimer′s disease

SUN Jialan, HUANG Shu, LAI Xiaoyin, ZHU Wei, HU Rongguo, LI Longxuan

Department of Neurology, Gongli Hospital, Pudong New Area, Shanghai 200135, China

Received:2018-06-11 Online:2018-08-25 Published:2018-08-25

摘要/Abstract

摘要： 目的：探讨外周血淋巴细胞中G蛋白偶联受体激酶5（G protein-coupled receptor kinase-5,GRK5）水平与阿尔茨海默病（Alzheimer′s disease, AD）患者认知障碍间的相关性。方法：选择2015年1月至2017年12月期间我院住院和门诊的AD患者,根据简易智能精神状态量表（mini-mental state examination, MMSE）评分分为轻度AD（27例）和中-重度AD组（37例）,另选择30名健康体检者作为对照。应用酶联免疫吸附试验（enzyme linked immunosorbent assay, ELISA）检测所有研究对象外周血淋巴细胞中的GRK5蛋白表达情况,进行组间比较,并分析外周血GRK水平与患者疾病间的相关性。结果：轻度[（7.58±0.50） ng/mL]及中-重度AD组[（4.37±0.26） ng/mL]外周血淋巴细胞中GRK5水平与对照组[（15.65±0.74） ng/mL]相比,差异均有统计学意义（P<0.001）;轻度AD组与中-重度AD组相比,差异亦有统计学意义（P<0.001）;线性回归分析显示,外周血淋巴细胞中GRK5蛋白水平与MMSE评分呈显著正相关（R²=0.678 1）。结论：AD患者存在GRK5水平下降;外周血淋巴细胞GRK5可能作为早期诊断AD的一种新型生物标志物。

关键词: 阿尔茨海默病, 认知功能损害, G蛋白偶联受体激酶5

Abstract: Objective: To investigate the correlation between G protein-coupled receptor kinase-5 (GRK5) level in peripheral lymphocytes and the degree of cognitive impairment in Alzheimer's disease(AD) patients. Methods: A total of 64 patients with AD during January 2015 to December 2017 were enrolled and divided into two groups, including 27 cases of mild dementia and 37 cases of moderate-to-severe dementia; 30 healthy subjects were served as controls. Enzyme-linked immunosorbent assay(ELISA) was used to detect the GRK5 level in peripheral lymphocytes of AD patients and controls. Results: The GRK5 level in mild and moderate-to-severe AD patients was significantly lower than that in controls (P<0.001). Moreover, the GRK5 level in moderate-to-severe AD patients was significantly lower than that in the mild AD patients (P<0.001). Linear regression analyses revealed a strong positive correlation between the GRK5 level and the Mini Mental State Examination (MMSE) score (P<0.001). Conclusions: Our data suggestes that low level of GRK5 is existed in AD patients and mirrored the impairment of cognitive state. GRK5 level of peripheral blood lymphocytes might be used as a novel biomarker for the early diagnosis of AD patients.

Key words: Alzheimer's disease, Cognitive impairment, G protein-coupled receptor kinase-5

中图分类号:

R741

孙家兰, 黄澍, 来小音, 朱玮, 胡荣郭, 李龙宣. 阿尔茨海默病患者外周血淋巴细胞中G蛋白偶联受体激酶5水平与疾病及程度间相关性探讨[J]. 诊断学理论与实践, 2018, 17(04): 419-422.

SUN Jialan, HUANG Shu, LAI Xiaoyin, ZHU Wei, HU Rongguo, LI Longxuan. Correlation between lymphocyte G-protein-coupled receptor kinase-5 level and cognitive impairment in patients with Alzheimer′s disease[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(04): 419-422.

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阿尔茨海默病患者外周血淋巴细胞中G蛋白偶联受体激酶5水平与疾病及程度间相关性探讨

Correlation between lymphocyte G-protein-coupled receptor kinase-5 level and cognitive impairment in patients with Alzheimer′s disease

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