程序性细胞死亡因子5在活动性类风湿关节炎患者中的水平变化

doi:10.16150/j.1671-2870.2018.04.019

摘要/Abstract

摘要： 目的：探究程序性细胞死亡因子5（programmed cell death factor 5,PDCD5）在类风湿关节炎（rheumatoid arthritis, RA）患者中的表达情况,并探讨PDCD5在RA发病中的可能作用。方法：采用酶联免疫吸附试验检测36例活动性RA患者和35例骨关节炎（osteoarthritis,OA）患者的血清和关节液中的PDCD5、白细胞介素6（interleukin 6,IL-6）水平,另以33名健康人的血清PDCD5水平作为对照（对照组）,比较3组间以上各指标水平的差异。同时再检测RA患者的C反应蛋白（C reaction protein, CRP）、红细胞沉降率（erythrocyte sedimentation rate, ESR）、类风湿因子（rheumatoid factor,RF）和环瓜氨酸肽（cyclic citrullinated peptide, CCP）,记录其肿胀关节数（swollen joint count,SJC）、疼痛关节数（tender joints count, TJC）,作为疾病活动指数参考指标。结果：RA组的血清PDCD5水平[（32.47±12.79） ng/mL]显著高于OA组[（12.79±9.84） ng/mL]及对照组[（18.40±18.97） ng/mL];RA组关节液中的PDCD5水平[（47.75±21.94） ng/mL]亦高于OA组[（19.33±11.25） ng/mL]。RA患者血清中的PDCD5水平与IL-6水平呈负相关（r=-0.431）,但在关节液中未观察到两者间具有相关性。Pearson分析显示,RA患者血清中的PDCD5水平与CRP、ESR、SJC、TJC呈负相关（R值分别为-0.523、-0.701、-0.845、-0.943）,但与CCP及RF水平间无统计学相关性。结论：活动性RA患者的血清及关节液中PDCD5的水平升高,而其表达失调可能与IL-6水平相关。

关键词: 类风湿关节炎, 程序性细胞死亡因子5, 白细胞介素6

Abstract: Objective: To investigate the expression of programmed cell death factor 5 ( PDCD5) in patients with rheumatoid arthritis (RA) and to explore its potential role in the pathogenesis of RA. Methods: A total of 36 patients with RA and 35 patients with osteoarthritis (OA) were enrolled, and 33 healthy people were served as controls. PDCD5 and IL-6 levels in serum as well as the levels in synovial fluid were measured by enzyme-linked immunosorbent assay (ELISA). Indices including C reaction protein (CRP), erythrocyte sedimentation rate(ESR), rheumatoid factor(RF）and cyclic citrullinated protein (CCP）were also detected, while count of swollen joints (SJC) and count of tender joints (TJC) served as reference of disease activity were recorded. Results: Compared with OA patients and controls, RA patients had a higher serum level of PDCD5 [（32.47±12.79） ng/mL vs （12.79±9.84） ng/mL in OA and (18.40±18.97） ng/mL in controls]. PDCD5 levels in synovial fluid of RA patients were also higher than those of OA patients. Furthermore, serum level of PDCD5 in RA patients was negatively correlated with IL-6 level in serum, however, no correlation was observed in synovial fluid. Pearson analysis showed that serum PDCD5 level in RA was negatively correlated with CRP, ESR, TJC and SJC (R=-0.523, -0.701, -0.845, -0.943, respectively), but had no correlation with RF and CCP. Conclusions: Serum and synovial levels of PDCD5 could reflect the activity of RA, and its abnormal expression may be associated with level of IL-6.

Key words: Rheumatoid arthritis, Programmed cell death factor 5, Interleukin 6

中图分类号:

R593.2

袁昳玮, 顾娟芳, 费张丽, 杨明峰, 王艺文, 王宏智. 程序性细胞死亡因子5在活动性类风湿关节炎患者中的水平变化[J]. 诊断学理论与实践, 2018, 17(04): 453-456.

YUAN Yiwei, GU Juanfang, FEI Zhangli, YANG Mingfeng, WANG Yiwen, WANG Hongzhi. Level of programmed cell death factor 5 in patients with active rheumatoid arthritis[J]. Journal of Diagnostics Concepts & Practice, 2018, 17(04): 453-456.

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