转移相关基因1蛋白在子宫颈病变组织中的表达及临床意义

doi:10.16150/j.1671-2870.2017.03.020

摘要/Abstract

摘要： 目的: 研究转移相关基因1(metastasis associated gene 1, MTA1)蛋白在子宫颈(以下简称宫颈)不同病变组织中的表达情况及其临床意义。方法: 应用SP免疫组织化学染色法检测40例宫颈癌、45例宫颈上皮内瘤变(cervical intraepithelial neoplasias, CIN)、30例慢性宫颈炎和20例正常宫颈组织的石蜡标本中MTA1蛋白的表达情况,并对其表达水平与宫颈癌的病理类型、分化程度、临床分期及是否有淋巴结转移等生物学行为间的相关性进行分析。结果: MTA1的高表达率在正常宫颈组(0)、CIN组(57.78%)、宫颈癌组(87.50%)间的比较,差异有统计学意义(P<0.05),而慢性宫颈炎组(10%)与正常宫颈组相比,MTA1高表达率差异无统计学意义(P>0.05)。慢性宫颈炎组与CIN组、宫颈癌组3组间两两相比,MTA1高表达率差异均具有统计学意义(P<0.05)。MTA1的高表达率在鳞状上皮内低度病变(low grade squamous intraepithelial lesion, LSIL)、鳞状上皮内高度病变(high grade squamous intraepithelial lesion, HSIL)、宫颈鳞癌3组间比较,也具有显著差异(P<0.05)。MTA1的高表达率与病理类型无关(P>0.05),而与宫颈癌的分化程度、淋巴结转移及临床分期有关(P<0.05)。结论: MTA1在正常宫颈组织及慢性宫颈炎组织中呈低表达,而MTA1在CIN及宫颈癌中呈现高表达,其高表达率与宫颈癌的分化程度、淋巴结转移及临床分期有关。

关键词: 宫颈癌, 宫颈上皮内瘤变, 转移相关基因1, 免疫组织化学

Abstract: Objective: To investigate the expression and significance of metastasis associated gene 1(MTA1) protein in normal cervix, chronic cervicitis, cervical intraepithelial neoplasia (CIN) and cervical cancer tissues. Methods: Biopsy paraffin sections including 40 cases of cervical cancer,45 cases of CIN, 30 cases of chronic cervicitis, 20 cases of normal cervix were investigated. Expression of MTA1 protein were examined by immunohistochemistry, and results were analyzed statistically using semiquantitative method. The correlation of MTA1 expression with biological behavior of cervical cancer was explored, including pathological classification, histological grade, clinical stage and lymph node metastasis. Results: Rates of high expression of MTA1 differed between cervical cancer, CIN and normal cervix (P<0.05). There was no difference in rate of high expression of MTA1 between chronic cervicitis and normal cervix(P>0.05). There were significant di-fferences in high expression rate of MTA1 between low grade squamous intraepithelial lesion(LSIL), high grade squamous intraepithelial lesion(HSIL) and cervical cancer tissues( P<0.05). Rate of high expression of MTA1 was correlated with degree of differentiation, lymph node metastasis and clinical stage, and was not associated with pathological type. Conclusions: MTA1 expression is low in normal and chronic cervicitis issue, and is high in CIN and cervical cancer tissue. Rate of high expression of MTA1 is in close association with the differentiation degree, lymph node metastasis and clinical stage of cervical cancer.

Key words: Cervical cancer, Cervical intraepithelial neoplasias, Metastasis associated gene 1, Immunohistochemical methods

中图分类号:

R737.33

顾青, 潘晓林, 赵艳. 转移相关基因1蛋白在子宫颈病变组织中的表达及临床意义[J]. 诊断学理论与实践, 2017, 16(03): 333-337.

GU Qing, PAN Xiaolin, ZHAO Yan. Expression and significance of MTA1 in different cervical lesions[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(03): 333-337.

参考文献

[1] Kumar JV1, Doval DC, Rao R, et al. A retrospective study of patients with locally advanced cancer of the cervix treated with neoadjuvant chemotherapy followed by radical surgery[J]. Int J Gynecol Cancer,2009,19(3):417-422.
[2] Abraham J, Stenger M.Cobas HPV test for first-line screening for cervical cancer[J]. J Community Support Oncol,2014,12(5):156-157.
[3] Yoo YG, Kong G, Lee MO.Metastasis-associated protein 1 enhances stability of hypoxia-inducible factor-1alpha protein by recruiting histone deacetylase 1[J]. EMBO J,2006,25(6):1231-1241.
[4] Pencil SD, Toh Y, Nicolson GL.Candidate metastasis-associated genes of the rat 13762NF mammary adenocarcinoma[J]. Breast Cancer Res Treat,1993,25(2):165-174.
[5] Manavathi B, Kumar R.Metastasis tumor antigens, an emerging family of multifaceted master coregulators[J]. J Biol Chem,2007,282(3):1529-1533.
[6] 田菁, 肖会廷, 冯慧, 等. MTA1在卵巢癌中的表达及其对卵巢癌细胞侵袭转移影响的研究[J]. 中国肿瘤临床,2012,39(21):1619-1622.
[7] 徐彩华, 沈振亚, 黄皓岳, 等. MTA1在非小细胞肺癌组织中的表达及意义[J]. 江苏医药,2014, (13):1530-1532.
[8] 张恩, 陈武科, 李斌, 等. 转移相关基因MTA1及CD44V9mRNA在乳腺癌中的表达及其临床意义[J]. 现代肿瘤医学,2010,18(5):896-898.
[9] 张金龙, 王海娟, 刘健, 等. 结肠癌组织中MTA1的表达水平及定位与患者预后的相关性研究[J]. 癌症进展,2012,10(3):216-220.
[10] 侯二文, 王禾, 马建军, 等. MTA1在膀胱癌的表达及其与预后的相关性[J]. 现代泌尿外科杂志,2014,19(4):263-266.
[11] 黄春蓓, 胡国华, 董蒲江, 等. 喉癌中MTA1分布特点及其与 MMP-9、VEGF-C蛋白表达的相关性分析[J]. 重庆医学,2014,43(26):3418-3421.
[12] 张山岭, 郝权, 孙和熙, 等. MTA1表达与子宫内膜癌相关性研究[J]. 中国肿瘤临床,2012,39(4):208-211.
[13] 石克, 吕新全. p16、Ki-67双染在子宫颈上皮内病变病理诊断和分级中的应用[J]. 诊断学理论与实践,2016,16(6):602-607.
[14] 韩肖燕, 钱海利, 林晨, 等. 宫颈癌中MTA1蛋白的表达及临床意义[J]. 实用妇产科杂志,2011,27(7):511-514.
[15] 符丽华, 熊小英. MTA1基因在宫颈癌组织中的表达及其临床意义[J]. 中国妇幼健康研究,2006,17(5):349-351.