A study on correlation between multiple sclerosis and Epstein-Barr virus and IL-17/IL-23

doi:10.16150/j.1671-2870.2017.01.017

Abstract

Abstract: Objective: To study the expression of Epstein-Barr virus (EBV) autoantibodies and IL-17, IL-23 in cerebrospinal fluid of patients with multiple sclerosis (MS) and to define the relationship between IL-17/IL-23 and pathogenesis of MS for hoping to provide a diagnostic reference for clinical practice. Methods: Seventy-two patients with neurological diseases in Ruijin Hospital were categorized into 2 groups: 29 cases with central nervous system autoimmune disease (study group) and 43 cases of non-central nervous system autoimmune disease (control group). Enzyme-linked immunosorbent assay (ELISA) was used to assess the levels of EBV-VCA-IgM, EBV-EBNA-1IgG, IL-17, IL-23 in CSF. Results: The expressions of EBV-EBNA-1IgG, EBV-VCA IgM and IL-23 in study group were significantly higher than those in control group (P<0.01). In study group, the expression of IL-23 in MS patients was significantly higher than that in control group（P<0.01), and the expression of IL-17 in MS patients was higher than that in control group（P<0.05）. Conclusions: EBV is related to MS and probably contributes to the pathogenesis of MS. Meanwhile, the abnormal inflammatory axis of IL-17/IL-23 may also be related to the induction of MS. The study indicates a relationship of MS not only with viral infection, but also with a disordered autoimmune system.

Key words: Cerebrospinal fluid, Multiple Sclerosis, EBV autoantibodies, Interleukin, Enzyme-linked immunosorbent assay

CLC Number:

R593.2

MENG Jun, PENG Yibing, WANG Xuefeng, CHEN Ninan, ZHANG Dongqing. A study on correlation between multiple sclerosis and Epstein-Barr virus and IL-17/IL-23[J]. Journal of Diagnostics Concepts & Practice, 2017, 16(01): 88-92.

References

[1] Benoist C, Mathis D.Autoimmunity provoked by infection: how good is the case for T cell epitope mimicry?[J]. Nat Immunol,2001,2(9):797-801.
[2] Cross AH, Trotter JL, Lyons J.B cells and antibodies in CNS demyelinating disease[J]. J Neuroimmunol,2001,112(1-2):1-14.
[3] Archelos JJ, Storch MK, Hartung HP.The role of B cells and autoantibodies in multiple sclerosis[J]. Ann Neurol,2000,47(6):694-706.
[4] Segal BM.Th17 cells in autoimmune demyelinating di-sease[J]. Semin Immunopathol,2010,32(1):71-77.
[5] Chen Y, Langrish CL, McKenzie B, et al. Anti-IL-23 therapy inhibits multiple inflammatory pathways and ameliorates autoimmune encephalomyelitis[J]. J Clin Invest,2006,116(5):1317-1326.
[6] Marrie RA, Wolfson C, Sturkenboom MC, et al.Multiple sclerosis and antecedent infections: a case-control study[J]. Neurology,2000,54(12):2307-2310.
[7] Levin LI, Munger KL, Rubertone MV, et al.Multiple sclerosis and Epstein-Barr virus[J]. JAMA,2003,289(12):1533-1536.
[8] 单颖, 姜东, 王维忠, 等. EB病毒感染与多发性硬化关系的Meta分析[J]. 临床荟萃,2007,22(7):481-483.
[9] Bray PF, Luka J, Bray PF, et al.Antibodies against Epstein-Barr nuclear antigen (EBNA) in multiple sclerosis CSF, and two pentapeptide sequence identities between EBNA and myelin basic protein[J]. Neurology,1992,42(9):1798-1804.
[10] Noseworthy JH, Lucchinetti C, Rodriguez M, et al.Multiple sclerosis[J]. N Engl J Med,2000,343(13):938-952.
[11] Harrington LE, Hatton RD, Mangan PR, et al.Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages[J]. Nat Immunol,2005,6(11):1123-1132.
[12] Park H, Li Z, Yang XO, et al.A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17[J]. Nat Immunol,2005,6(11):1133-1141.
[13] Dalton DK, Wittmer S.Nitric-oxide-dependent and independent mechanisms of protection from CNS inflammation during Th1-mediated autoimmunity: evidence from EAE in iNOS KO mice[J]. J Neuroimmunol,2005,160(1-2):110-121.
[14] Li Y, Chu N, Hu A, et al. Increased IL-23 p19 expression in multiple sclerosis lesions and its induction in microglia[J]. Brain,2007 Feb,130(Pt 2):490-501.
[15] Tesmer LA, Lundy SK, Sarkar S, et al.Th17 cells in human disease[J]. Immunol Rev,2008,223:87-113.
[16] Matusevicius D, Kivisäkk P, He B, et al.Interleukin-17 mRNA expression in blood and CSF mononuclear cells is augmented in multiple sclerosis[J]. Mult Scler,1999,5(2):101-104.
[17] Komiyama Y, Nakae S, Matsuki T, et al.IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis[J]. J Immunol,2006,177(1):566-573.