Journal of Diagnostics Concepts & Practice ›› 2019, Vol. 18 ›› Issue (1): 77-81.doi: 10.16150/j.1671-2870.2019.01.015

• Original articles • Previous Articles     Next Articles

Application of microsatellite polymorphism and repetitive sequence-based PCR in genotyping of Candida tropicalis

WANG Guoqing1, LI Zhen2, PENG Yibing1()   

  1. 1. Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
    2. Clinical Laboratory, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2018-06-25 Online:2019-02-25 Published:2019-02-25
  • Contact: PENG Yibing E-mail:pyb9861@sina.com

Abstract:

Objective: To evaluate the application of microsatellite polymorphism in genotyping of Candida tropicalis. Methods: From August 2014 to November 2015, 50 clinical isolates of Candida tropicalis were collected form 4 hospitals, including Ruijin Hospital, Children’s Hospital, the First People’s Hospital and Zhongshan Hospital. Primers Ca21, Ca22, Com21 were used in pairs to find the best suitable pairs for the repetitive extragenic palindromic polymerase chain reaction (REP-PCR) genotyping. Three microsatellite markers Ctrm1, Ctrom10, Ctrm12 were used to analyze the microsatellite polymorphism. Finally, the discriminatory results of the two genotyping methods were compared. Result: The combination of Ca21-Com21 primers had the best effect in REP-PCR genotyping. Seven REP-PCR types A-G were found in 50 isolates of Candida tropicalis. The index of discriminatory power was 0.75. The 50 isolates were classified into 30 genotypes by the microsatellites polymorphism. The index of discriminatory power was 0.97. Conclusions: Microsatellite polymorphism is a simple and rapid method for molecular typing with higher discrimination power than REP-PCR. Therefore, microstellite polymorphism is the preferred choice in clinical laboratories.

Key words: Candida tropicalis, Genotype, Repetitive extragenic palindromicpolymerase chain reaction, Microsatellite polymorphism

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