Effect of laser-assisted hatching with complete removal of zona pellucida on the outcome of frozen single blastocyst transfer

doi:10.16150/j.1671-2870.2019.03.017

Abstract

Abstract:

Objective: To study the effect of laser-assisted hatching with complete removal of zona pellucida on pregnancy outcomes such as pregnancy rate and embryo implantation rate of frozen single blastocyst transfer. Methods: The cases of 200 cycles of single blastocyst transfer in Shanghai Towako hospital from September 2016 to August 2018 were enrolled and were divided into two groups randomly. Partially thinned zona pellucida was performed in the control group and complete removal of zona pellucida was performed in the study group. The general information of the patients including age, body mass index (BMI), cause of infertility, basic FSH level, etc, good quality embryo rate, cleavage rate, blastocyst formation rate, pregnancy rate, embryo implantation rate, monozygotic twins rate and abortion rate were observed and compared. Results: The age, BMI, cause of infertility in the study group were not significantly different from those of the control group (P>0.05). There were also no significant differences in good quality embryo rate, cleavage rate and blastocyst formation rate (P>0.05) between the two groups, and the pregnancy rate and embryo implantation rate in the study group were significantly higher than those in the control group (P<0.05), and the rates of monozygotic twins and abortion were significantly lower than those of the control group (P<0.05). Conclusions: Complete removal of zona pellucida before frozen blastocyst transfer can be an effective method to improve the success rate of single blastocyst transfer.

Key words: Complete removal of zona pellucida, Laser-assisted hatching, Frozen single blastocyst transfer, Clinical pregnancy rate, Embryo implantation rate

CLC Number:

R725.8

HUANG Ya, XU Li, GE Fengju, WU Yunlong, CHEN Yaning, QI Xiujuan. Effect of laser-assisted hatching with complete removal of zona pellucida on the outcome of frozen single blastocyst transfer[J]. Journal of Diagnostics Concepts & Practice, 2019, 18(03): 334-339.

Figures/Tables 4

References 24

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组别	周期数	平均年龄（岁）	BMI(kg/m²)	FSH(IU/mL)	内膜厚度（mm）	不孕原因（周期）（n=98）
组别	周期数	平均年龄（岁）	BMI(kg/m²)	FSH(IU/mL)	内膜厚度（mm）	输卵管因素	男方因素	排卵障碍	双方因素
对照组	98	34.3±1.6	21.4±2.2	7.65±1.2	8.53±1.81	41	15	11	31
研究组	98	34.5±1.7	21.1±2.0	7.56±1.0	8.68±1.68	42	12	11	33
P值	/	>0.05	>0.05	>0.05	>0.05	>0.05	>0.05	>0.05	>0.05

组别	周期数	卵裂率	优胚率	囊胚形成率
对照组	98	95.8	54.8	49.8
研究组	98	96.6	55.6	50.2
P值	/	>0.05	>0.05	>0.05

组别	临床妊娠率（%）	胚胎种植率（%）	单卵双胎率（%）	流产率（%）
对照组（周期数98个）	53.06（52/98）	59.18（58/98）	11.54（6/52）	11.54（6/52）
研究组（周期数98个）	62.24（61/98）	63.27（62/98）	1.64（1/61）	6.56（4/61）
P值	<0.05	<0.05	<0.01	<0.05