Journal of Diagnostics Concepts & Practice ›› 2022, Vol. 21 ›› Issue (01): 52-61.doi: 10.16150/j.1671-2870.2022.01.011
• Original articles • Previous Articles Next Articles
XU Fei1,2, YIN Mingyue1, WANG Wei1, DONG Zhiya1, LU Wenli1, YU Yi1, WANG Xinqiong1, WANG Junqi1, XIAO Yuan1()
Online:
2022-02-25
Published:
2022-02-25
Contact:
XIAO Yuan
E-mail:xy11438@rjh.com.cn
CLC Number:
XU Fei, YIN Mingyue, WANG Wei, DONG Zhiya, LU Wenli, YU Yi, WANG Xinqiong, WANG Junqi, XIAO Yuan. Metagenomic analysis of gut microbiota and antibiotic resistome in girls with precocious puberty[J]. Journal of Diagnostics Concepts & Practice, 2022, 21(01): 52-61.
指标 | CPP组(n=11) | PT组(n=10) | NC组(n=8) | P值 |
---|---|---|---|---|
年龄(岁) | 8.1±1.2 | 7.5±0.8 | 7.2±0.5 | 0.098 |
身高(cm) | 133.2±12.1 | 127.1±7.8 | 122.9±4.0 | 0.062 |
体重(kg) | 29.9±7.1 | 27.0±4.9 | 23.9±2.3 | 0.075 |
BMI(kg/m2) | 16.6±2.3 | 16.6±2.1 | 15.9±1.7 | 0.687 |
靶身高(cm) | 160.1±3.7 | 158.5±2.6 | 161.1±4.9 | 0.346 |
骨龄(years) | 9.8±1.5 | 8.2±2.1 | - | 0.053 |
基线LH(IU/L) | 1.9±2.0 | 0.6±1.1 | - | 0.016 |
LH峰值(IU/L) | 9.6(6.1~21.5) | 3.5(2.3~4.9) | - | <0.001 |
基线FSH(IU/L) | 3.3(2.5~5.8) | 2.6(1.3~3.0) | - | 0.324 |
FSH峰值(IU/L) | 8.5(7.4~16.7) | 13.5(9.4~17.6) | - | 0.032 |
LH/FSH | 1.1(0.8~2.1) | 0.2(0.2~0.4) | - | <0.001 |
[1] |
Eckert-Lind C, Busch AS, Petersen JH, et al. Worldwide Secular Trends in Age at Pubertal Onset Assessed by Breast Development Among Girls: A Systematic Review and Meta-analysis[J]. JAMA Pediatr, 2020, 174(4):e195881.
doi: 10.1001/jamapediatrics.2019.5881 URL |
[2] | Lopez J, Deoraj A. Effect of Gut Microbiome on Obesity Can Influence Precocious Puberty (CPP)[C]. 2015. |
[3] |
Dalby MJ, Ross AW, Walker AW, et al. Dietary Uncoupling of Gut Microbiota and Energy Harvesting from Obesity and Glucose Tolerance in Mice[J]. Cell Rep, 2017, 21(6):1521-1533.
doi: 10.1016/j.celrep.2017.10.056 URL |
[4] |
Heras V, Castellano JM, Fernandois D, et al. Central Ceramide Signaling Mediates Obesity-Induced Precocious Puberty[J]. Cell Metab, 2020, 32(6):951-966,e8.
doi: 10.1016/j.cmet.2020.10.001 URL |
[5] |
Pruden A, Pei R, Storteboom H, et al. Antibiotic resistance genes as emerging contaminants: studies in northern Colorado[J]. Environ Sci Technol, 2006, 40(23):7445-7450.
pmid: 17181002 |
[6] |
Fassarella M, Blaak EE, Penders J, et al. Gut microbiome stability and resilience: elucidating the response to perturbations in order to modulate gut health[J]. Gut, 2021, 70(3):595-605.
doi: 10.1136/gutjnl-2020-321747 pmid: 33051190 |
[7] |
Cox LM, Yamanishi S, Sohn J, et al. Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences[J]. Cell, 2014, 158(4):705-721.
doi: 10.1016/j.cell.2014.05.052 URL |
[8] |
Wang H, Wang N, Wang B, et al. Antibiotics detected in urines and adipogenesis in school children[J]. Environ Int, 2016, 89-90:204-211.
doi: 10.1016/j.envint.2016.02.005 URL |
[9] | White JR, Nagarajan N, Pop M. Statistical methods for detecting differentially abundant features in clinical metagenomic samples[J]. PLoS Comput Biol, 2009, 5(4):e1000352. |
[10] |
McArthur AG, Waglechner N, Nizam F, et al. The comprehensive antibiotic resistance database[J]. Antimicrob Agents Chemother, 2013, 57(7):3348-3357.
doi: 10.1128/AAC.00419-13 pmid: 23650175 |
[11] | Alcock BP, Raphenya AR, Lau TTY, et al. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database[J]. Nucleic Acids Res, 2020, 48(D1):D517-D525. |
[12] |
Riva A, Borgo F, Lassandro C, et al. Pediatric obesity is associated with an altered gut microbiota and discordant shifts in Firmicutes populations[J]. Environ Microbiol, 2017, 19(1):95-105.
doi: 10.1111/1462-2920.13463 URL |
[13] |
Liu R, Hong J, Xu X, et al. Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention[J]. Nat Med, 2017, 23(7):859-868.
doi: 10.1038/nm.4358 URL |
[14] |
Dong G, Zhang J, Yang Z, et al. The Association of Gut Microbiota With Idiopathic Central Precocious Puberty in Girls[J]. Front Endocrinol (Lausanne), 2020, 10:941.
doi: 10.3389/fendo.2019.00941 URL |
[15] | 周莎莎, 刘春杰, 李嫔. 肠道微生物及代谢产物对中枢性性早熟的影响[C]. 2020.第十九届全国儿科内分泌代谢遗传病会议 |
Zhou S S, Liu C J, Li B. Effects of intestinal microorganisms and metabolites on central precocious puberty[C]. 2020. The 19th National Conference on Pediatric Endocrine Metabolic Genetic Diseases. | |
[16] |
Yuan X, Chen R, Zhang Y, et al. Gut microbiota: effect of pubertal status[J]. BMC Microbiol, 2020, 20(1):334.
doi: 10.1186/s12866-020-02021-0 pmid: 33143658 |
[17] |
Hollister EB, Riehle K, Luna RA, et al. Structure and function of the healthy pre-adolescent pediatric gut microbiome[J]. Microbiome, 2015, 3:36.
doi: 10.1186/s40168-015-0101-x pmid: 26306392 |
[18] |
Wang M, Zhang Y, Miller D, et al. Microbial Reconstitution Reverses Early Female Puberty Induced by Maternal High-fat Diet During Lactation[J]. Endocrinology, 2020, 161(2):bqz041.
doi: 10.1210/endocr/bqz041 URL |
[19] | Li Y, Shen L, Huang C, et al. Altered nitric oxide induced by gut microbiota reveals the connection between central precocious puberty and obesity[J]. Clin Transl Med, 2021, 11(2):e299. |
[20] |
Liu YX, Qin Y, Chen T, et al. A practical guide to amplicon and metagenomic analysis of microbiome data[J]. Protein Cell, 2021, 12(5):315-330.
doi: 10.1007/s13238-020-00724-8 URL |
[21] |
Boolchandani M, D'Souza AW, Dantas G. Sequencing-based methods and resources to study antimicrobial resistance[J]. Nat Rev Genet, 2019, 20(6):356-370.
doi: 10.1038/s41576-019-0108-4 pmid: 30886350 |
[22] |
Feng J, Li B, Jiang X, et al. Antibiotic resistome in a large-scale healthy human gut microbiota deciphered by metagenomic and network analyses[J]. Environ Microbiol, 2018, 20(1):355-368.
doi: 10.1111/1462-2920.14009 pmid: 29194931 |
[23] | Milanović V, Osimani A, Aquilanti L, et al. Occurrence of antibiotic resistance genes in the fecal DNA of healthy omnivores, ovo-lacto vegetarians and vegans[J/OL]. Mol Nutr Food Res, 2017-06-09 [2021-12-12]. https://pubmed.ncbi.nlm.nih.gov/28464483/. |
[24] |
Korpela K, de Vos WM. Antibiotic use in childhood alters the gut microbiota and predisposes to overweight[J]. Microb Cell, 2016, 3(7):296-298.
doi: 10.15698/mic2016.07.514 pmid: 28357367 |
[25] |
Gosalbes MJ, Vallès Y, Jiménez-Hernández N, et al. High frequencies of antibiotic resistance genes in infants′ meconium and early fecal samples[J]. J Dev Orig Health Dis, 2016, 7(1):35-44.
doi: 10.1017/S2040174415001506 URL |
[26] |
Lu N, Hu Y, Zhu L, et al. DNA microarray analysis reveals that antibiotic resistance-gene diversity in human gut microbiota is age related[J]. Sci Rep, 2014, 4:4302.
doi: 10.1038/srep04302 URL |
[27] | 隋倩雯, 张俊亚, 魏源送, 等. 畜禽养殖过程抗生素使用与耐药病原菌及其抗性基因赋存的研究进展[J]. 生态毒理学报, 2015, 10(5):20-34. |
Sui Q W, Zhang J Y, Wei Y S, et al. Research Progress on antibiotic use, drug resistant pathogens and occurrence of resistance genes in livestock and poultry breeding[J]. J ecotoxicol, 2015, 10(5):20-34. | |
[28] |
Dibner JJ, Richards JD. Antibiotic growth promoters in agriculture: history and mode of action[J]. Poult Sci, 2005, 84(4):634-643.
doi: 10.1093/ps/84.4.634 URL |
[29] |
Castanon JI. History of the use of antibiotic as growth promoters in European poultry feeds[J]. Poult Sci, 2007, 86(11):2466-2471.
doi: 10.3382/ps.2007-00249 URL |
[30] |
Gough EK, Moodie EE, Prendergast AJ, et al. The impact of antibiotics on growth in children in low and middle income countries: systematic review and meta-analysis of randomised controlled trials[J]. BMJ, 2014, 348:g2267.
doi: 10.1136/bmj.g2267 URL |
[31] |
Niewold TA. The nonantibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis[J]. Poult Sci, 2007, 86(4):605-609.
doi: 10.1093/ps/86.4.605 URL |
[32] |
Naik OA, Shashidhar R, Rath D, et al. Characterization of multiple antibiotic resistance of culturable microorganisms and metagenomic analysis of total microbial diversity of marine fish sold in retail shops in Mumbai, India[J]. Environ Sci Pollut Res Int, 2018, 25(7):6228-6239.
doi: 10.1007/s11356-017-0945-7 URL |
[33] |
Danko D, Bezdan D, Afshin EE, et al. A global metagenomic map of urban microbiomes and antimicrobial resistance[J]. Cell, 2021, 184(13):3376-3393,e17.
doi: 10.1016/j.cell.2021.05.002 URL |
[1] | MA Xiaoyu, YANG Yuanyan, LU Wenli, NI Jihong, WANG Junqi, CHEN Ye, QIN Xueyan, DONG Zhiya, WANG Wei. The quantitative measurement of morning voided urinary gonadotropin in diagnosis of rapidly progressive central precocious puberty in prepubertal girls [J]. Journal of Diagnostics Concepts & Practice, 2020, 19(05): 516-520. |
[2] | ZHU Xiaolei, CHEN Lu, LU Wenli, LIU Yan, YAN Fuhua, WANG Wei, DONG Zhiya. Radiological findings on pituitary MRI in central precocious puberty [J]. Journal of Diagnostics Concepts & Practice, 2019, 18(03): 286-290. |
[3] | MA Xiaoyu, LU Wenli, NI Jihong, WANG Junqi, CHEN Ye, Qin Xueyan, DONG Zhiya, WANG Wei. Value of quantitative assay of urinary LH and FSH in differentiating classic from transitional premature thelarche [J]. Journal of Diagnostics Concepts & Practice, 2019, 18(03): 291-295. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||