外科理论与实践 ›› 2021, Vol. 26 ›› Issue (06): 543-549.doi: 10.16139/j.1007-9610.2021.06.017
收稿日期:
2020-05-09
出版日期:
2021-11-25
发布日期:
2022-07-27
通讯作者:
毛志海
E-mail:zhihaimao@163.com
基金资助:
LIU Shiguang, ZHAO Jingkun, LU Aiguo, MAO Zhihai()
Received:
2020-05-09
Online:
2021-11-25
Published:
2022-07-27
Contact:
MAO Zhihai
E-mail:zhihaimao@163.com
摘要:
目的:分析CXCL5(C-X-C motif ligand 5)蛋白与程序性死亡配体1(programmed death ligand 1,PD-L1)在结肠直肠癌组织中的表达,并探讨两者与临床特征的关系及预后价值。方法:收集2010年至2011年行腹腔镜切除手术的结肠直肠癌病人肿瘤组织及癌旁正常组织标本,共78例。同时对所有病例进行临床资料收集及预后随访。标本制备组织芯片,行免疫组织化学染色。采用免疫危险评分标准对CXCL5、PD-L1表达进行评分,分析其表达与各项临床病理参数关系。最后分析CXCL5、PD-L1表达与结肠直肠癌病人预后的关系。结果:结肠直肠癌组织中CXCL5、PD-L1表达均高于癌旁正常组织,与肿瘤直径、TNM分期相关。Cox单因素回归分析显示,TNM分期、CXCL5高表达、PD-L1高表达是结肠直肠癌病人总生存率的预后危险因素。多因素回归分析显示,TNM分期和PD-L1高表达是结肠直肠癌病人总生存率的独立预后危险因素。CXCL5与PD-L1均高表达的病人预后最差。结论:CXCL5与PD-L1联合可对结肠直肠癌病人进行危险度分层,预测病人预后。
中图分类号:
刘诗光, 赵敬坤, 陆爱国, 毛志海. 趋化因子CXCL5和程序性死亡配体 1在结肠直肠癌组织的表达与病人预后的关系[J]. 外科理论与实践, 2021, 26(06): 543-549.
LIU Shiguang, ZHAO Jingkun, LU Aiguo, MAO Zhihai. Expression of chemokine CXCL5 and programme death ligand 1 in colorectal cancer tissues are associated with prognosis of patients[J]. Journal of Surgery Concepts & Practice, 2021, 26(06): 543-549.
图1
基于结肠直肠癌病人生存数据的X-tile分析显示CXCL5和PD-L1免疫组织化学染色评分的cut-off值 A、D:以cut-off值为界将队列分成两个子集;X轴表示从低到高(从左到右)的所有潜在cut-off值,定义为低表达子集,Y轴表示从高到低(从上到下)的潜在cut-off值,定义为高表达子集。红色的cut-off 值表示与生存负相关,而绿色着色表示正相关,最佳cut-off值由软件计算而得(相当于AUC曲线下最大面积时的cut-off值);B、E:以直方图显示最佳cut-off值,绿色部分表示低表达,灰色部分表示高表达;C、F:以Kaplan-Meier曲线显示最佳cut-off值。
表1
结肠直肠癌组织中CXCL5、PD-L1表达与病人临床特征的关系
临床特征 | CXCL5表达 | 统计学数值 | P值 | PD-L1表达 | 统计学数值 | P值 | ||
---|---|---|---|---|---|---|---|---|
高 | 低 | 高 | 低 | |||||
总计 | 48 | 30 | 37 | 41 | ||||
性别 | 1.086 | 0.297 | 1.236 | 0.266 | ||||
男 | 23 | 18 | 17 | 24 | ||||
女 | 25 | 12 | 20 | 17 | ||||
年龄(岁) | 0.194 | 0.660 | 0.360 | 0.549 | ||||
<65 | 28 | 19 | 21 | 26 | ||||
≥65 | 20 | 11 | 16 | 15 | ||||
组织学 | 3.534 | 0.171 | 2.982 | 0.225 | ||||
管状 | 38 | 28 | 29 | 37 | ||||
黏液 | 9 | 2 | 7 | 4 | ||||
乳头状 | 1 | 0 | 1 | 0 | ||||
肿瘤直径(cm) | 3.945 | 0.047 | 4.029 | 0.045 | ||||
≥5 | 27 | 10 | 22 | 15 | ||||
<5 | 21 | 20 | 15 | 26 | ||||
TNM分期 | 2.735 | 0.003 | 11.733 | 0.008 | ||||
Ⅰ | 5 | 10 | 4 | 10 | ||||
Ⅱ | 21 | 12 | 11 | 16 | ||||
Ⅲ | 16 | 8 | 16 | 15 | ||||
Ⅳ | 6 | 0 | 6 | 0 |
表2
结肠直肠癌病人单因素与多因素Cox回归分析
参数 | 总生存率 | ||
---|---|---|---|
HR | 95% CI | P值 | |
单因素分析 | |||
年龄(岁)(<65比≥65) | 1.003 | 0.410~2.454 | 0.995 |
性别(女比男) | 1.812 | 0.740~4.433 | 0.193 |
组织学 | |||
乳头状 | 1.000 | .. | 0.222 |
管状比乳头状 | 0.164 | 0.021~1.272 | 0.084 |
黏液比乳头状 | 0.198 | 0.020~1.948 | 0.165 |
TNM分期 | |||
Ⅲ+Ⅳ比Ⅰ+Ⅱ | 0.181 | 0.060~0.541 | 0.002 |
肿瘤直径(<5 cm比≥5 cm) | 0.537 | 0.219~1.314 | 0.173 |
CXCL5(低表达比高表达) | 0.229 | 0.067~0.783 | 0.019 |
PD-L1(低表达比高表达) | 0.397 | 0.164~0.958 | 0.040 |
多因素分析 | |||
TNM分期 | |||
Ⅲ+Ⅳ比Ⅰ+Ⅱ | 0.235 | 0.075~0.734 | 0.013 |
CXCL5(低表达比高表达) | 0.786 | 0.202~3.063 | 0.728 |
PD-L1(低表达比高表达) | 0.156 | 0.042~0.584 | 0.006 |
TNM分期 | |||
Ⅲ+Ⅳ比Ⅰ+Ⅱ | 0.234 | 0.076~0.721 | 0.011 |
CXCL5(低表达比高表达) | 0.345 | 0.098~1.215 | 0.098 |
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