长链非编码RNA TUG1在结肠直肠癌组织中的表达与分析

doi:10.16139/j.1007-9610.2018.04.020

摘要/Abstract

摘要： 目的检测结肠直肠癌组织长链非编码RNA牛磺酸上调基因1（taurine upregulated gene1,TUG1）的表达,并探讨其与结肠直肠癌临床病理因素及预后的相关性及临床意义。方法采用实时定量聚合酶链反应检测106例结肠直肠癌病人的癌组织与相应的癌旁组织（距癌组织边缘>5 cm）TUG1表达。分析TUG1表达与临床病理的相关性以及TUG1表达与结肠直肠癌预后的相关性。术后随访3~60个月。结果 TUG1 在结肠直肠癌组织中表达明显高于癌旁组织（P=0.003）,表达水平与肿瘤直径（P<0.001）、CEA水平（P=0.049）以及TNM分期（P=0.005）密切相关。TUG1高表达的结肠直肠癌病人,生存率低于低表达病人（P=0.002 5）。多因素分析表明,TUG1可作为结肠直肠癌病人的独立预后因素。结论 TUG1在结肠直肠癌的发生发展中起重要作用,可作为反映结肠直肠癌生物学行为的有效指标。

关键词: 结肠直肠癌, 牛磺酸上调基因1, 实时定量聚合酶链反应, 预后

Abstract: Objective To investigate the expression of long non-coding RNA TUG1 (taurine upregulated gene 1) in co-lorectal cancer tissues and the correlation between TUG1 expression and clinicopathological factors in patients with colorectal cancer. Methods Real-time quantitative polymerase chain reaction was used to detect TUG1 expression in cancer tissues and paracancerous tissues (>5 cm from the edge of cancer tissues) of 106 patients with colorectal cancer. The correlations between TUG1 expression and clinicopathological factors, and between TUG1 expression and the survivals of patients with colorectal cancer were analyzed. The patients were followed up from 3 to 60 months. Results In comparison with the expression of TUG1 in adjacent normal tissues, the expression of TUG1 in colorectal cancer tissues was significantly higher (P=0.003). We found that high expression of TUG1 related with tumor diameter (P<0.001), serum CEA (P=0.049) and TNM stage (P=0.005) significantly. Higher expression of TUG1 in patients with colorectal cancer had shorter overall survival times compared with the patients with lower expression of PUG1 (P=0.0025). Multivariate regression analysis showed that TUG1 expression could be an independent prognostic factor in patients with colorectal cancer. Conclusions TUG1 might play an important role in the development of colorectal cancer and may be used as predictor of prognosis for colorectal cancer.

Key words: Colorectal cancer, Taurine upregulated gene 1, Real-time quantitative polymerase chain reaction, Prognosis

中图分类号:

周勇, 崔荣, 石兴耀. 长链非编码RNA TUG1在结肠直肠癌组织中的表达与分析[J]. 外科理论与实践, 2018, 23(04): 374-378.

ZHOU Yong, CUI Rong, SHI Xingyao. Analysis of long non-coding RNA TUG1 expression in colorectal cancer[J]. Journal of Surgery Concepts & Practice, 2018, 23(04): 374-378.

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